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    Fusion reporter gene for bacterial luciferase
    1.
    发明授权
    Fusion reporter gene for bacterial luciferase 失效
    融合报告基因的细菌荧光素酶

    公开(公告)号：US5196524A

    公开(公告)日：1993-03-23

    申请号：US294170

    申请日：1989-01-06

    申请人： Gary D. Gustafson ,  Thomas D. Ingolia ,  Gretchen Kirchner ,  Jean L. Roberts

    发明人： Gary D. Gustafson ,  Thomas D. Ingolia ,  Gretchen Kirchner ,  Jean L. Roberts

    IPC分类号： C12N9/02 ,  C12N15/80 ,  C12N15/82 ,  C12N15/85

    CPC分类号： C12N9/0071 ,  C12N15/80 ,  C12N15/8209 ,  C12N15/85 ,  C12Y114/14003 ,  C07K2319/00 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/70 ,  C12N2830/702 ,  C12N2840/20

    摘要： Novel fusion reporter genes, fusion reporter proteins, and an improved reporter system for measuring the relative activity of a promoter sequence. A luxAB fusion gene of the present invention is particularly useful as a reporter gene and is derived from the fusion of a luxA gene and a luxB gene from Vibrio harveyi. The gene products of the luxA and luxB genes are the .alpha.- and .beta.-subunits, respectively, of a bacterial luciferase. A fusion protein encoded by a luxAB fusion gene is a single active protein and is particularly useful as a reporter protein having luciferase activity. An advantage of such a reporter system to assay gene expression in many cells which contain FMNH.sub.2, such as bacterial and yeast cells, is that an immediate and quantitative assessment of gene expression may be made from real-time light measurements using intact cells.

    摘要翻译： 新型融合报告基因，融合报道蛋白，以及用于测量启动子序列相对活性的改进的报道系统。 本发明的luxAB融合基因特别可用作报告基因，并且来源于来自哈氏弧菌的luxA基因和luxB基因的融合。 luxA和luxB基因的基因产物分别是细菌荧光素酶的α和β亚基。 由luxAB融合基因编码的融合蛋白是单一活性蛋白，并且特别可用作具有荧光素酶活性的报道蛋白。 这种报告系统在许多含有FMNH2的细胞（如细菌和酵母细胞）中测定基因表达的优点是可以使用完整的细胞从实时光测量中进行基因表达的即时和定量评估。