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公开(公告)号：US20110223606A1
公开(公告)日：2011-09-15
申请号：US13068727
申请日：2011-05-17
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solubilize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下从保存的临床样品中有效溶解核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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公开(公告)号：US07968327B2
公开(公告)日：2011-06-28
申请号：US11893356
申请日：2007-08-14
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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公开(公告)号：US20080050746A1
公开(公告)日：2008-02-28
申请号：US11893356
申请日：2007-08-14
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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