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公开(公告)号：US20110223606A1
公开(公告)日：2011-09-15
申请号：US13068727
申请日：2011-05-17
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solubilize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下从保存的临床样品中有效溶解核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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公开(公告)号：US07968327B2
公开(公告)日：2011-06-28
申请号：US11893356
申请日：2007-08-14
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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公开(公告)号：US20080050746A1
公开(公告)日：2008-02-28
申请号：US11893356
申请日：2007-08-14
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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公开(公告)号：US20110171644A1
公开(公告)日：2011-07-14
申请号：US12930041
申请日：2010-12-23
申请人： Yuling Luo , Yunqing Ma , Gary McMaster , Quan Ngoc Nguyen , Frank Witney , Wen Yang
发明人： Yuling Luo , Yunqing Ma , Gary McMaster , Quan Ngoc Nguyen , Frank Witney , Wen Yang
CPC分类号： C12Q1/6874 , C12Q1/6832 , C12Q1/6834 , C12Q1/6837 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： Methods of capturing two or more nucleic acids simultaneously from a single sample are provided. Different nucleic acids are captured through cooperative hybridization events on different subsets of particles or at different selected positions on a spatially addressable solid support. Methods of capturing one or more long nucleic acids and methods of capturing one or more nucleic acid for sequencing are also provided. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了从单个样品同时捕获两种或更多种核酸的方法。 通过在不同的颗粒子集上或在空间上可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还提供了捕获一种或多种长核酸的方法和捕获一种或多种核酸进行测序的方法。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20080176242A1
公开(公告)日：2008-07-24
申请号：US11983216
申请日：2007-11-06
申请人： Gary McMaster , Yunqing Ma
发明人： Gary McMaster , Yunqing Ma
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2563/103 , C12Q2537/162 , C12Q2531/113
摘要： This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.
摘要翻译： 本发明提供在两级扩增中检测感兴趣的核酸的方法，组合物和系统。 两步扩增开始于作为第二次核酸扩增或测定的靶底物的扩增寡聚物的第一非酶积累。 可以使用两个或更多个扩增寡聚体，以基于独特的检测信号或唯一的信号位置，通过去卷积来允许两个或多个感兴趣的核酸的多重扩增。
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公开(公告)号：US07927798B2
公开(公告)日：2011-04-19
申请号：US11543752
申请日：2006-10-04
申请人： Zhi Zheng , Yuling Luo , Gary McMaster
发明人： Zhi Zheng , Yuling Luo , Gary McMaster
CPC分类号： C12Q1/6816 , C12Q1/6837 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313
摘要： Methods of detecting one or more nucleic acids from whole blood or plasma are provided. The nucleic acids are captured on a solid support and detected. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供从全血或血浆中检测一种或多种核酸的方法。 将核酸捕获在固体支持物上并检测。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20070161015A1
公开(公告)日：2007-07-12
申请号：US11543752
申请日：2006-10-04
申请人： Zhi Zheng , Yuling Luo , Gary McMaster
发明人： Zhi Zheng , Yuling Luo , Gary McMaster
CPC分类号： C12Q1/6816 , C12Q1/6837 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313
摘要： Methods of detecting one or more nucleic acids from whole blood or plasma are provided. The nucleic acids are captured on a solid support and detected. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供从全血或血浆中检测一种或多种核酸的方法。 将核酸捕获在固体支持物上并检测。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20110256536A1
公开(公告)日：2011-10-20
申请号：US13066727
申请日：2011-04-21
申请人： Gary McMaster , Yunqing Ma
发明人： Gary McMaster , Yunqing Ma
IPC分类号： C12Q1/68 , G01N27/447 , G01N33/559 , G01N33/53 , C07H21/00
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2563/103 , C12Q2537/162 , C12Q2531/113
摘要： This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.
摘要翻译： 本发明提供在两级扩增中检测感兴趣的核酸的方法，组合物和系统。 两步扩增开始于作为第二次核酸扩增或测定的靶底物的扩增寡聚物的第一非酶积累。 可以使用两个或更多个扩增寡聚体，以基于独特的检测信号或唯一的信号位置，通过去卷积来允许两个或多个感兴趣的核酸的多重扩增。
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公开(公告)号：US08470535B2
公开(公告)日：2013-06-25
申请号：US13066727
申请日：2011-04-21
申请人： Gary McMaster , Yunqing Ma
发明人： Gary McMaster , Yunqing Ma
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2563/103 , C12Q2537/162 , C12Q2531/113
摘要： This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.
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公开(公告)号：US07951539B2
公开(公告)日：2011-05-31
申请号：US12587523
申请日：2009-10-07
申请人： Gary McMaster , Yunqing Ma
发明人： Gary McMaster , Yunqing Ma
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2563/103 , C12Q2537/162 , C12Q2531/113
摘要： This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.
摘要翻译： 本发明提供在两级扩增中检测感兴趣的核酸的方法，组合物和系统。 两步扩增开始于作为第二次核酸扩增或测定的靶底物的扩增寡聚物的第一非酶积累。 可以使用两个或更多个扩增寡聚体，以基于独特的检测信号或唯一的信号位置，通过去卷积来允许两个或多个感兴趣的核酸的多重扩增。
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