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公开(公告)号：US5710251A
公开(公告)日：1998-01-20
申请号：US495558
申请日：1995-12-18
申请人： Gary Vellekamp , Susan Cannon-Carlson , John Tang
发明人： Gary Vellekamp , Susan Cannon-Carlson , John Tang
CPC分类号： C07K14/5428
摘要： Disclosed is a method for purifying Interleukin-10 (IL-10). The method is comprised of subjecting an IL-10 containing solution to cation exchange chromatography, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration chromatography. The present invention is also comprised of a process for separating different IL-10 dimers present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography. The present invention is also comprised of a process for separating variants of a protein differing in an N-terminal amino acid sequence present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography.
摘要翻译： PCT No.PCT / US94 / 01909 Sec。 371 1995年12月18日第 102（e）日期1995年12月18日PCT 1994年3月3日PCT公布。 出版物WO94 / 20525 日期1994年9月15日公开是一种纯化白细胞介素-10（IL-10）的方法。 该方法包括将含IL-10的溶液进行阳离子交换层析，阴离子交换层析，羟基磷灰石层析和凝胶过滤层析。 本发明还包括通过对蛋白质级分进行羟基磷灰石色谱法分离存在于蛋白质级分中的不同IL-10二聚体的方法。 本发明还包括通过使蛋白质级分进行羟基磷灰石色谱法分离存在于蛋白质级分中的N末端氨基酸序列不同的蛋白质的变体的方法。
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公开(公告)号：US5328989A
公开(公告)日：1994-07-12
申请号：US26942
申请日：1993-03-05
申请人： Gary Vellekamp , Susan Cannon-Carlson , John Tang
发明人： Gary Vellekamp , Susan Cannon-Carlson , John Tang
CPC分类号： C07K14/5428
摘要： A method for purifying Interleukin-10 (Il-10) from a eukaryotic cell culture medium. The method is comprised of subjecting IL-10 containing cell culture medium to cation exchange chromatography, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration chromatography. The present invention is also comprised of a process for separating different IL-10 dimers present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography. The present invention is also comprised of a process for separating variants of a protein differing in an N-terminal amino acid sequence present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography.
摘要翻译： 从真核细胞培养基中纯化白细胞介素-10（Il-10）的方法。 该方法包括将含IL-10的细胞培养基进行阳离子交换层析，阴离子交换层析，羟基磷灰石层析和凝胶过滤层析。 本发明还包括通过对蛋白质级分进行羟基磷灰石层析来分离存在于蛋白质级分中的不同IL-10二聚体的方法。 本发明还包括通过使蛋白质级分进行羟基磷灰石色谱法分离存在于蛋白质级分中的N末端氨基酸序列不同的蛋白质的变体的方法。
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公开(公告)号：US20060105456A1
公开(公告)日：2006-05-18
申请号：US11326275
申请日：2006-01-05
CPC分类号： C12N7/00 , C07K14/4746 , C12N2710/10351
摘要： Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by conventional purification techniques are further reduced to provide a highly pure adenovirus preparation.
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公开(公告)号：US08697045B2
公开(公告)日：2014-04-15
申请号：US13218929
申请日：2011-08-26
申请人： Seoju Lee , David C. Wylie , Susan Cannon-Carlson
发明人： Seoju Lee , David C. Wylie , Susan Cannon-Carlson
CPC分类号： A61K38/2066 , A61K47/60 , C07K14/5428 , C07K2319/31
摘要： Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selective for the N-terminus on one subunit of IL-10 with little or no formation of monomeric IL-10. The method also provides a substantially homogenous population of mono-PEG-IL-10.
摘要翻译： 提供了通过接头与IL-10的单个氨基酸残基上的一个或多个聚乙二醇（PEG）分子缀合的白细胞介素-10（IL-10）及其制备方法。 该方法产生稳定的单聚乙二醇化IL-10，其保留IL-10活性，其中聚乙二醇化对IL-10的一个亚基上的N末端具有选择性，几乎或不形成单体IL-10。 该方法还提供基本均匀的单PEG-IL-10群体。
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公开(公告)号：US20110305665A1
公开(公告)日：2011-12-15
申请号：US13218929
申请日：2011-08-26
申请人： Seoju Lee , David C. Wylie , Susan Cannon-Carlson
发明人： Seoju Lee , David C. Wylie , Susan Cannon-Carlson
CPC分类号： A61K38/2066 , A61K47/60 , C07K14/5428 , C07K2319/31
摘要： Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selective for the N-terminus on one subunit of IL-10 with little or no formation of monomeric IL-10. The method also provides a substantially homogenous population of mono-PEG-IL-10.
摘要翻译： 提供了通过接头与IL-10的单个氨基酸残基上的一个或多个聚乙二醇（PEG）分子缀合的白细胞介素-10（IL-10）及其制备方法。 该方法产生稳定的单聚乙二醇化IL-10，其保留IL-10活性，其中聚乙二醇化对IL-10的一个亚基上的N末端具有选择性，几乎或不形成单体IL-10。 该方法还提供基本均匀的单PEG-IL-10群体。
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公开(公告)号：US06281337B1
公开(公告)日：2001-08-28
申请号：US09441653
申请日：1999-11-12
申请人： Susan Cannon-Carlson , Andres Frei , Seoju Lee , Roland Mengisen , Marcio Voloch , David C. Wylie
发明人： Susan Cannon-Carlson , Andres Frei , Seoju Lee , Roland Mengisen , Marcio Voloch , David C. Wylie
IPC分类号： C07K100
CPC分类号： C07K14/56
摘要： The present invention provides methods for isolating adjunct isoforms of proteins and converting them to the desired protein. In preferred embodiments, the present invention contemplates the use of acid solutions or zinc solutions to cleave a chemical group from a desired protein. In further preferred embodiments, the present invention contemplates the oxidation of reduced sulfhydryl groups with cleavage of chemical groups to form a functional desired protein.
摘要翻译： 本发明提供了用于分离蛋白质的附加同种型并将其转化为所需蛋白质的方法。 在优选的实施方案中，本发明考虑使用酸溶液或锌溶液从期望的蛋白质切割化学基团。 在进一步优选的实施方案中，本发明考虑到通过化学基团切割形成功能性所需蛋白质来还原巯基的氧化。
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公开(公告)号：US20060210534A1
公开(公告)日：2006-09-21
申请号：US11440962
申请日：2006-05-25
申请人： Seoju Lee , David Wylie , Susan Cannon-Carlson
发明人： Seoju Lee , David Wylie , Susan Cannon-Carlson
IPC分类号： A61K38/20 , C07K14/545
CPC分类号： A61K38/2066 , A61K47/60 , C07K14/5428 , C07K2319/31
摘要： Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selective for the N-terminus on one subunit of IL-10 with little or no formation of monomeric IL-10. The method also provides a substantially homogenous population of mono-PEG-IL-10.
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