公开(公告)号：US20180057862A1

公开(公告)日：2018-03-01

申请号：US15811364

申请日：2017-11-13

Applicant: GEN-PROBE INCORPORATED

Inventor： Jennifer J. BUNGO ,  James J. HOGAN ,  Reinhold B. POLLNER ,  Marie K. HUDSPETH ,  Shannon K. KAPLAN ,  Elizabeth M. MARLOWE

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/686 ,  Y02A50/451

Abstract: Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Legionella pneumophila 16S or 23S rRNA sequences or DNA encoding 16S or 23S rRNA. Methods are disclosed for detecting the presence of L. pneumophila in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 16S rRNA sequence or DNA encoding the 16S rRNA sequence, or of a 23S rRNA sequence or DNA encoding the 23S rRNA sequence to produce a detectable amplification product.

公开(公告)号：US20160222439A1

公开(公告)日：2016-08-04

申请号：US14927785

申请日：2015-10-30

Applicant: Gen-Probe Incorporated

Inventor： Michael R. RESHATOFF, JR. ,  Jennifer J. BUNGO ,  Shannon K. KAPLAN ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/158 ,  Y02A50/451

Abstract: The disclosed invention is related to compositions, kits and methods comprising one or more oligomers targeting 16S rRNA target nucleic acid from Campylobacter species jejuni, coli and/or lari. Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.

Abstract translation: 所公开的发明涉及包含一种或多种靶向来自空肠弯曲杆菌，大肠杆菌和/或乳杆菌的16S rRNA靶核酸的低聚物的组合物，试剂盒和方法。 组合物包括扩增寡聚物，检测探针低聚物和/或靶捕获低聚物。 试剂盒和方法包括这些寡聚物中的至少一种。

公开(公告)号：US20180073059A1

公开(公告)日：2018-03-15

申请号：US15699502

申请日：2017-09-08

Applicant: Gen-Probe Incorporated

Inventor： Shannon K. KAPLAN ,  Kristin W. LIVEZEY ,  Michael M. BECKER ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.

公开(公告)号：US20140227695A1

公开(公告)日：2014-08-14

申请号：US14215407

申请日：2014-03-17

Applicant: Gen-Probe Incorporated

Inventor： Michael R. RESHATOFF ,  Edgar O. ONG ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q1/689 ,  C12Q2304/00 ,  Y02A50/451

Abstract: Provided are compositions, kits, and methods for the identification of Salmonella. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.

Abstract translation: 提供用于鉴定沙门氏菌的组合物，试剂盒和方法。 在某些方面和实施方案中，组合物，试剂盒和方法可提供与特异性，灵敏度和检测速度有关的改进。

公开(公告)号：US20170211132A1

公开(公告)日：2017-07-27

申请号：US15424052

申请日：2017-02-03

Applicant: GEN-PROBE INCORPORATED

Inventor： Michael R. RESHATOFF ,  Kristin W. LIVEZEY ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6816 ,  C12Q1/6865

Abstract: Provided are compositions, kits, and methods for the identification of Listeria. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.

公开(公告)号：US20160060685A1

公开(公告)日：2016-03-03

申请号：US14938630

申请日：2015-11-11

Applicant: Gen-Probe Incorporated

Inventor： Shannon K. KAPLAN ,  Kristin W. LIVEZEY ,  Michael M. BECKER ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.

Abstract translation: 用于扩增和检测来自Mollicutes类的各种物种的核酸的组合物，反应混合物，试剂盒和方法。 23S rRNA或其基因的特定区域已被鉴定为怀疑含有至少一种Mollicutes的样品的核酸扩增反应的优选靶标。 一些寡聚体包括标签区，靶闭合区，启动子序列和/或结合部分。 样品可以来自怀疑含有Mollicutes类物种的任何来源。 优选的样品来源包括生物反应器，细胞系，细胞培养物和制药产品。

公开(公告)号：US20150111212A1

公开(公告)日：2015-04-23

申请号：US14581919

申请日：2014-12-23

Applicant: Gen-Probe Incorporated

Inventor： Shannon K. KAPLAN ,  Kristin LIVEZEY ,  Jennifer J. BUNGO ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689

Abstract: The present invention provides compositions, methods and kits for the species-specific detection of pseudomonas aeruginosa.

Abstract translation: 本发明提供了用于种类特异性检测铜绿假单胞菌的组合物，方法和试剂盒。

公开(公告)号：US20140287411A1

公开(公告)日：2014-09-25

申请号：US14268107

申请日：2014-05-02

Applicant: Gen-Probe Incorporated

Inventor： Michael R. RESHATOFF ,  Kristin W. LIVEZEY ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6816 ,  C12Q1/6865

Abstract: Provided are compositions, kits, and methods for the identification of Listeria. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.

Abstract translation: 提供用于鉴定李斯特菌的组合物，试剂盒和方法。 在某些方面和实施方案中，组合物，试剂盒和方法可以提供与特异性，灵敏度和检测速度有关的改进。