公开(公告)号：US20180010172A1

公开(公告)日：2018-01-11

申请号：US15708039

申请日：2017-09-18

Applicant: GEN-PROBE INCORPORATED

Inventor： Michael M. BECKER ,  Kristin W. LIVEZEY ,  Wai-Chung LAN

IPC: C12Q1/68

Abstract: The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in subsequent analysis, or present in the environment in which an assay is performed, are free of bacterial or other contaminating nucleic acids.

公开(公告)号：US20210024982A1

公开(公告)日：2021-01-28

申请号：US17061552

申请日：2020-10-01

Applicant: GEN-PROBE INCORPORATED

Inventor： Michael M. BECKER ,  Kristin W. LIVEZEY ,  Wai-Chung LAM

IPC: C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/686

Abstract: The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in subsequent analysis, or present in the environment in which an assay is performed, are free of bacterial or other contaminating nucleic acids.

公开(公告)号：US20180073059A1

公开(公告)日：2018-03-15

申请号：US15699502

申请日：2017-09-08

Applicant: Gen-Probe Incorporated

Inventor： Shannon K. KAPLAN ,  Kristin W. LIVEZEY ,  Michael M. BECKER ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.

公开(公告)号：US20160186249A1

公开(公告)日：2016-06-30

申请号：US15069645

申请日：2016-03-14

Applicant: GEN-PROBE INCORPORATED

Inventor： Michael M. BECKER ,  Kristin W. LIVEZEY ,  Wai-Chung LAM

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2525/155

Abstract: The present invention provides nucleic acid amplification systems and methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.

公开(公告)号：US20190024158A9

公开(公告)日：2019-01-24

申请号：US15069645

申请日：2016-03-14

Applicant: GEN-PROBE INCORPORATED

Inventor： Michael M. BECKER ,  Kristin W. LIVEZEY ,  Wai-Chung LAM

IPC: C12Q1/68

Abstract: The present invention provides nucleic acid amplification systems and methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.

公开(公告)号：US20170211132A1

公开(公告)日：2017-07-27

申请号：US15424052

申请日：2017-02-03

Applicant: GEN-PROBE INCORPORATED

Inventor： Michael R. RESHATOFF ,  Kristin W. LIVEZEY ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6816 ,  C12Q1/6865

Abstract: Provided are compositions, kits, and methods for the identification of Listeria. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.

公开(公告)号：US20160060685A1

公开(公告)日：2016-03-03

申请号：US14938630

申请日：2015-11-11

Applicant: Gen-Probe Incorporated

Inventor： Shannon K. KAPLAN ,  Kristin W. LIVEZEY ,  Michael M. BECKER ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.

Abstract translation: 用于扩增和检测来自Mollicutes类的各种物种的核酸的组合物，反应混合物，试剂盒和方法。 23S rRNA或其基因的特定区域已被鉴定为怀疑含有至少一种Mollicutes的样品的核酸扩增反应的优选靶标。 一些寡聚体包括标签区，靶闭合区，启动子序列和/或结合部分。 样品可以来自怀疑含有Mollicutes类物种的任何来源。 优选的样品来源包括生物反应器，细胞系，细胞培养物和制药产品。

公开(公告)号：US20140287411A1

公开(公告)日：2014-09-25

申请号：US14268107

申请日：2014-05-02

Applicant: Gen-Probe Incorporated

Inventor： Michael R. RESHATOFF ,  Kristin W. LIVEZEY ,  James J. HOGAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6816 ,  C12Q1/6865

Abstract: Provided are compositions, kits, and methods for the identification of Listeria. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.

Abstract translation: 提供用于鉴定李斯特菌的组合物，试剂盒和方法。 在某些方面和实施方案中，组合物，试剂盒和方法可以提供与特异性，灵敏度和检测速度有关的改进。