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公开(公告)号：US12013401B2
公开(公告)日：2024-06-18
申请号：US18142538
申请日：2023-05-02
发明人： Scot Randy Weinberger , Ronald Carl Orlando , Joshua Shane Sharp , Robert Wallace Egan , Jeffrey Jonathan Persoff
CPC分类号： G01N33/6803 , C23C18/14 , G01N21/59 , G01N21/631 , G01N21/6428 , H01J49/0036 , G01N2021/6439
摘要： Flash Photo-Oxidation Device and Higher Order Structural Analysis is employed for higher order structural analysis of biomolecules. Biomolecular higher order structure (HOS) results from the confounded superimposition of a biomolecule's secondary, tertiary, and quaternary structure and defines the manner in which a biomolecule presents itself and interacts with other biomolecules in living systems. A rapidly growing class of therapeutic drugs, known as biotherapeutics, comprises a variety of proteins, whose therapeutic properties are inherently linked and dependent upon their HOS. As such, HOS analysis of biotherapeutics is an important analytical requirement in the biopharmaceutical industry. The present invention provides new means and methods for the determination of biopharmaceutical HOS and associated conformation using improved devices and methodologies for flash photo-oxidation of proteins to determine their higher order biomolecular structure, and such is responsive to the increased demand for new and improved HOS analytical means in the biopharmaceutical industry.
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公开(公告)号：US12016688B2
公开(公告)日：2024-06-25
申请号：US17411855
申请日：2021-08-25
CPC分类号： A61B5/1455 , A61B5/14546 , G01N21/631 , G01N33/6803 , A61B2562/0233 , G01N21/53 , G01N21/645
摘要： Systems and methods of in vivo hydroxyl radical protein foot-printing are presented. These teachings may be used to, for example, study three-dimensional protein structure or bio-kinetics. Radical Dosimetry including an optional intrinsic standard is used on isolated intact cells. Real-time feedback based on an internal standard provides comparability between different experiments and in vivo analysis results in data that is representative of actual biological conditions.
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公开(公告)号：US12013400B2
公开(公告)日：2024-06-18
申请号：US17168472
申请日：2021-02-05
发明人： Scot Randy Weinberger , Ronald Carl Orlando , Joshua Shane Sharp , Robert Wallace Egan , Jeffrey Jonathan Persoff
CPC分类号： G01N33/6803 , C23C18/14 , G01N21/59 , G01N21/631 , G01N21/6428 , H01J49/0036 , G01N2021/6439
摘要： Flash Photo-Oxidation Device and Higher Order Structural Analysis is employed for higher order structural analysis of biomolecules. Biomolecular higher order structure (HOS) results from the confounded superimposition of a biomolecule's secondary, tertiary, and quaternary structure and defines the manner in which a biomolecule presents itself and interacts with other biomolecules in living systems. A rapidly growing class of therapeutic drugs, known as biotherapeutics, comprises a variety of proteins, whose therapeutic properties are inherently linked and dependent upon their HOS. As such, HOS analysis of biotherapeutics is an important analytical requirement in the biopharmaceutical industry. The present invention provides new means and methods for the determination of biopharmaceutical HOS and associated conformation using improved devices and methodologies for flash photo-oxidation of proteins to determine their higher order biomolecular structure, and such is responsive to the increased demand for new and improved HOS analytical means in the biopharmaceutical industry.
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公开(公告)号：US20210223255A1
公开(公告)日：2021-07-22
申请号：US17193913
申请日：2021-03-05
摘要： The three-dimensional structural analysis of pharmaceutical and/or biological molecules is performed by the reaction of OH radicals on the surfaces of the molecules of interest. Quantitation and/or completeness of the OH radicals are optionally measured using buffers intrinsic to the sample solutions as internal standards. Measurements of the reactions of these buffers with OH radicals provide an internal standard while avoiding the use of prior art internal standards that can have unwanted effects on the three-dimensional structures of interest.
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公开(公告)号：US20230296615A1
公开(公告)日：2023-09-21
申请号：US18142538
申请日：2023-05-02
发明人： Scot Randy Weinberger , Ronald Carl Orlando , Joshua Shane Sharp , Robert Wallace Egan , Jeffrey Jonathan Persoff
CPC分类号： G01N33/6803 , G01N21/59 , G01N21/6428 , H01J49/0036 , G01N21/631 , G01N2021/6439
摘要： Flash Photo-Oxidation Device and Higher Order Structural Analysis is employed for higher order structural analysis of biomolecules. Biomolecular higher order structure (HOS) results from the confounded superimposition of a biomolecule's secondary, tertiary, and quaternary structure and defines the manner in which a biomolecule presents itself and interacts with other biomolecules in living systems. A rapidly growing class of therapeutic drugs, known as biotherapeutics, comprises a variety of proteins, whose therapeutic properties are inherently linked and dependent upon their HOS. As such, HOS analysis of biotherapeutics is an important analytical requirement in the biopharmaceutical industry. The present invention provides new means and methods for the determination of biopharmaceutical HOS and associated conformation using improved devices and methodologies for flash photo-oxidation of proteins to determine their higher order biomolecular structure, and such is responsive to the increased demand for new and improved HOS analytical means in the biopharmaceutical industry.
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公开(公告)号：US20210378558A1
公开(公告)日：2021-12-09
申请号：US17411855
申请日：2021-08-25
IPC分类号： A61B5/1455 , A61B5/145
摘要： Systems and methods of in vivo hydroxyl radical protein foot-printing are presented. These teachings may be used to, for example, study three-dimensional protein structure or bio-kinetics. Radical Dosimetry including an optional intrinsic standard is used on isolated intact cells. Real-time feedback based on an internal standard provides comparability between different experiments and in vivo analysis results in data that is representative of actual biological conditions.
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公开(公告)号：US20210156869A1
公开(公告)日：2021-05-27
申请号：US17168472
申请日：2021-02-05
发明人： Scot Randy Weinberger , Ronald Carl Orlando , Joshua Shane Sharp , Robert Wallace Egan , Jeffrey Jonathan Persoff
摘要： Flash Photo-Oxidation Device and Higher Order Structural Analysis is employed for higher order structural analysis of biomolecules. Biomolecular higher order structure (HOS) results from the confounded superimposition of a biomolecule's secondary, tertiary, and quaternary structure and defines the manner in which a biomolecule presents itself and interacts with other biomolecules in living systems. A rapidly growing class of therapeutic drugs, known as biotherapeutics, comprises a variety of proteins, whose therapeutic properties are inherently linked and dependent upon their HOS. As such, HOS analysis of biotherapeutics is an important analytical requirement in the biopharmaceutical industry. The present invention provides new means and methods for the determination of biopharmaceutical HOS and associated conformation using improved devices and methodologies for flash photo-oxidation of proteins to determine their higher order biomolecular structure, and such is responsive to the increased demand for new and improved HOS analytical means in the biopharmaceutical industry.
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公开(公告)号：US20220080418A1
公开(公告)日：2022-03-17
申请号：US17532806
申请日：2021-11-22
发明人： Scot Randy Weinberger , Robert Wallace Egan , Jeffrey Johnathan Persoff , Emily Elizabeth Chea , Tan Huu Bui , David Allan Holman
摘要： Systems and methods of in vivo and in vitro radical protein foot-printing using an opto-fluidic array are presented. These teachings may be used to, for example, study three-dimensional protein structure or bio-kinetics. Radical dosimetry including an optional intrinsic standard is used. Real-time feedback based on an internal standard provides comparability between different experiments and in vivo and in vitro analysis results in data that is representative of actual biological conditions.
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公开(公告)号：US11181529B2
公开(公告)日：2021-11-23
申请号：US17193913
申请日：2021-03-05
摘要： The three-dimensional structural analysis of pharmaceutical and/or biological molecules is performed by the reaction of OH radicals on the surfaces of the molecules of interest. Quantitation and/or completeness of the OH radicals are optionally measured using buffers intrinsic to the sample solutions as internal standards. Measurements of the reactions of these buffers with OH radicals provide an internal standard while avoiding the use of prior art internal standards that can have unwanted effects on the three-dimensional structures of interest.
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公开(公告)号：US10816468B2
公开(公告)日：2020-10-27
申请号：US16316006
申请日：2018-05-25
发明人： Scot Randy Weinberger , Ronald Carl Orlando , Joshua Shane Sharp , Robert Wallace Egan , Jeffrey Jonathan Persoff
摘要： Flash Photo-Oxidation Device and Higher Order Structural Analysis is employed for higher order structural analysis of biomolecules. Biomolecular higher order structure (HOS) results from the confounded superimposition of a biomolecule's secondary, tertiary, and quaternary structure and defines the manner in which a biomolecule presents itself and interacts with other biomolecules in living systems. A rapidly growing class of therapeutic drugs, known as biotherapeutics, comprises a variety of proteins, whose therapeutic properties are inherently linked and dependent upon their HOS. As such, HOS analysis of biotherapeutics is an important analytical requirement in the biopharmaceutical industry. The present invention provides new means and methods for the determination of biopharmaceutical HOS and associated conformation using improved devices and methodologies for flash photo-oxidation of proteins to determine their higher order biomolecular structure, and such is responsive to the increased demand for new and improved HOS analytical means in the biopharmaceutical industry.
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