公开(公告)号：US20070259373A1

公开(公告)日：2007-11-08

申请号：US11809905

申请日：2007-05-31

申请人： Gena Whitney ,  Gregory Opiteck ,  Leah Garulacan ,  Chandra Ramanathan ,  Murray McKinnon ,  Kelly Bennett ,  Lauren Barber ,  Angela Cacace ,  Zenta Tsuchihashi ,  John Feder

发明人： Gena Whitney ,  Gregory Opiteck ,  Leah Garulacan ,  Chandra Ramanathan ,  Murray McKinnon ,  Kelly Bennett ,  Lauren Barber ,  Angela Cacace ,  Zenta Tsuchihashi ,  John Feder

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C07K14/705 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/5017 ,  G01N33/5023 ,  G01N33/5041 ,  G01N33/5044 ,  G01N33/5091 ,  G01N33/566 ,  G01N33/574 ,  G01N33/6884 ,  G01N33/74 ,  G01N2333/726 ,  G01N2500/00 ,  G01N2800/122

摘要： The present invention describes a G-protein coupled receptor (GPCR) family member newly identified as being modified, e.g., phosphorylated, and associated with tyrosine phosphorylated activation complexes, following exposure of cells to smoke from tobacco burning substances, namely, cigarette smoke. This GPCR protein is RAI-3, which was first found to be phosphorylated in cells treated with cigarette smoke and to be associated with other proteins activated in cigarette smoke treated cells by virtue of the present invention. Because cigarette smoke is considered to be a major causative factor of chronic obstructive pulmonary disease (COPD) and disorders and conditions related thereto, the RAI-3 protein is newly provided as a cellular drug target for screening, discovering, and identifying modulators for the treatment and/or prevention of COPD and its related disorders and conditions, such as emphysema and chronic bronchitis. In accordance with the present invention RAI-3 modulators, e.g., agonists and antagonists, can be used as therapeutics in the treatment of COPD and numerous other diseases and disorders that are associated with regulation of NF-κB and/or its associated or interacting signaling molecules. This invention further provides SNPs of RAI-3, e.g., for determining COPD association in individuals.

摘要翻译： 本发明描述了在将烟草暴露于烟草燃烧物质（即香烟烟雾）之后，新鉴定为被修饰，例如磷酸化并与酪氨酸磷酸化活化复合物相关的G-蛋白偶联受体（GPCR）家族成员。 该GPCR蛋白是RAI-3，其首先被发现在用香烟烟雾处理的细胞中被磷酸化并且与通过本发明在香烟烟雾处理的细胞中活化的其它蛋白质相关联。 由于香烟烟雾被认为是慢性阻塞性肺疾病（COPD）的主要致病因素及与之相关的病症和病症，所以RAI-3蛋白质作为筛选，发现和鉴定调节剂治疗的细胞药物靶点被新提供 和/或预防COPD及其相关疾病和病症如肺气肿和慢性支气管炎。 根据本发明，RAI-3调节剂（例如激动剂和拮抗剂）可用作治疗COPD和许多与调节NF-κB和/或其相关或相互作用的信号相关的其它疾病和病症 分子。 本发明还提供RAI-3的SNP，例如用于确定个体中的COPD关联。

公开(公告)号：US20070117141A1

公开(公告)日：2007-05-24

申请号：US11640775

申请日：2006-12-18

申请人： Juan Perez-Villar ,  Han Chang ,  Wen-Pin Yang ,  Yuli Wu ,  Gena Whitney ,  Steven Kanner

发明人： Juan Perez-Villar ,  Han Chang ,  Wen-Pin Yang ,  Yuli Wu ,  Gena Whitney ,  Steven Kanner

IPC分类号： C12Q1/68 ,  A61K38/17 ,  C07K14/705 ,  C07K16/28 ,  C07H21/04 ,  C12P21/06

CPC分类号： C07K14/47 ,  A01K2217/05 ,  Y10S435/81

摘要： The present invention describes a newly discovered full-length polynucleotide encoding an SH2 domain-containing adapter protein, called human MIST, cloned, isolated and identified from a human spleen cDNA library. Also described are the MIST polypeptide sequence, expression vectors, host cells, agonists, antagonists, antisense molecules, and antibodies related to the polynucleotide and/or polypeptide of the present invention. Novel splice variant forms of human MIST are provided. Methods for screening for modulators, particularly inhibitors, of the MIST protein and use of the human MIST polynucleotide and polypeptide for therapeutics and diagnostics are described.

摘要翻译： 本发明描述了一种新发现的全长多核苷酸，其编码含有SH2结构域的衔接蛋白，称为人MIST，从人脾cDNA文库克隆，分离和鉴定。 还描述了与本发明的多核苷酸和/或多肽相关的MIST多肽序列，表达载体，宿主细胞，激动剂，拮抗剂，反义分子和抗体。 提供人类MIST的新型剪接变体形式。 描述了用于筛选MIST蛋白的调节剂，特别是抑制剂的方法以及人MIST多核苷酸和多肽用于治疗和诊断的用途。

公开(公告)号：US20070264662A1

公开(公告)日：2007-11-15

申请号：US11789322

申请日：2007-04-24

申请人： Donald Jackson ,  Stanley Krystek ,  Donna Bassolino ,  Rolf-Peter Ryseck ,  Thomas Nelson ,  Gena Whitney

发明人： Donald Jackson ,  Stanley Krystek ,  Donna Bassolino ,  Rolf-Peter Ryseck ,  Thomas Nelson ,  Gena Whitney

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  C12P21/06 ,  C12N9/12

CPC分类号： G01N33/6893 ,  C07K14/47 ,  G01N33/564 ,  G01N33/57407 ,  G01N33/57426 ,  G01N33/57484 ,  G01N2800/065 ,  G01N2800/102 ,  G01N2800/104 ,  G01N2800/12 ,  G01N2800/122 ,  G01N2800/205 ,  G01N2800/24 ,  G01N2800/245

摘要： The invention relates to the identification and cloning of a two novel full-length human SLAP-2 variants and their encoded polypeptides, hSLAP-2v3 and hSLAP-2v4. hSLAP-2v3 and hSLAP-2v4 are members of the SLAP family of adapter proteins and are involved in the negative regulation of intracellular T-cell signal transduction. The invention further relates to the use of these novel hSLAP-2v3 and hSLAP-2v4 polynucleotides and their encoded polypeptides as targets for therapeutic intervention in immunological and inflammatory disorders, autoimmune diseases, pulmonary diseases, proliferative immune disorders, and cancer.

摘要翻译： 本发明涉及两种新型全长人SLAP-2变体及其编码多肽hSLAP-2v3和hSLAP-2v4的鉴定和克隆。 hSLAP-2v3和hSLAP-2v4是SLAP家族接头蛋白的成员，并且参与细胞内T细胞信号转导的负调控。 本发明还涉及这些新型hSLAP-2v3和hSLAP-2v4多核苷酸及其编码多肽作为免疫学和炎症性疾病，自身免疫疾病，肺部疾病，增殖性免疫病症和癌症中的治疗性干预的靶标的用途。

公开(公告)号：US20060193858A1

公开(公告)日：2006-08-31

申请号：US11429515

申请日：2006-05-05

申请人： Han Chang ,  Wen-Pin Yang ,  Yuli Wu ,  Gena Whitney ,  Juan Perez-villar ,  Steven Kanner

发明人： Han Chang ,  Wen-Pin Yang ,  Yuli Wu ,  Gena Whitney ,  Juan Perez-villar ,  Steven Kanner

IPC分类号： A61K48/00 ,  C07H21/04 ,  C12P21/06 ,  A61K39/395 ,  C07K16/28

CPC分类号： C07K14/47 ,  A61K38/00

摘要： The present invention describes a newly discovered full-length polynucleotide encoding an SH2/SH3 domain-containing adapter protein, called hSLAP-2, cloned, isolated and identified. Also described are the hSLAP-2 polypeptide sequence, expression vectors, host cells, agonists, antagonists, anti-sense molecules, and antibodies related to the polynucleotide and/or polypeptide of the present invention. Methods for screening for modulators, particularly inhibitors, of the hSLAP-2 protein and use of the hSLAP-2 polynucleotide and polypeptide for therapeutics and diagnostics are described.

摘要翻译： 本发明描述了新发现的编码含有SH2 / SH3结构域的衔接蛋白（称为hSLAP-2）的克隆，分离和鉴定的全长多核苷酸。 还描述了与本发明的多核苷酸和/或多肽相关的hSLAP-2多肽序列，表达载体，宿主细胞，激动剂，拮抗剂，反义分子和抗体。 描述了用于筛选hSLAP-2蛋白的调节剂，特别是抑制剂的方法以及hSLAP-2多核苷酸和多肽用于治疗和诊断的方法。