公开(公告)号：US20210309989A1

公开(公告)日：2021-10-07

申请号：US17213611

申请日：2021-03-26

Applicant: GENENTECH, INC.

Inventor： Michael Wilson LAIRD ,  Shahram MISAGHI ,  Amy SHEN ,  Anthony TOMLINSON ,  Inn Huam YUK

IPC: C12N15/10 ,  C12N5/071

Abstract: The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.

公开(公告)号：US20250101411A1

公开(公告)日：2025-03-27

申请号：US18823189

申请日：2024-09-03

Applicant: Genentech, Inc.

Inventor： Michael Wilson LAIRD ,  Shahram MISAGHI ,  Amy SHEN ,  Anthony TOMLINSON ,  Inn Huam YUK

IPC: C12N15/10 ,  C12N5/071

Abstract: The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.