公开(公告)号：US20230348953A1

公开(公告)日：2023-11-02

申请号：US18043568

申请日：2021-08-31

Applicant: GENENTECH, INC.

Inventor： Li YI ,  Adithi Chandrasekhara BHARGAVA ,  Inn Huam YUK

IPC: C12Q1/44

CPC classification number: C12Q1/44 ,  G01N2333/918

Abstract: The present disclosure provides compositions, methods, and kits for detecting lipolytic activity. In some embodiments, the composition comprises an aqueous assay sample and an organic solvent, wherein the organic solvent comprises 4-methylumbellif-erryl caprylate (MU-C8). Also provided herein are methods for determining the stability of a protein preparation.

公开(公告)号：US20210309989A1

公开(公告)日：2021-10-07

申请号：US17213611

申请日：2021-03-26

Applicant: GENENTECH, INC.

Inventor： Michael Wilson LAIRD ,  Shahram MISAGHI ,  Amy SHEN ,  Anthony TOMLINSON ,  Inn Huam YUK

IPC: C12N15/10 ,  C12N5/071

Abstract: The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.

公开(公告)号：US20250101411A1

公开(公告)日：2025-03-27

申请号：US18823189

申请日：2024-09-03

Applicant: Genentech, Inc.

Inventor： Michael Wilson LAIRD ,  Shahram MISAGHI ,  Amy SHEN ,  Anthony TOMLINSON ,  Inn Huam YUK

IPC: C12N15/10 ,  C12N5/071

Abstract: The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.

公开(公告)号：US20210238644A1

公开(公告)日：2021-08-05

申请号：US17172528

申请日：2021-02-10

Applicant: GENENTECH, INC.

Inventor： Inn Huam YUK ,  Patrick Daniel AHYOW ,  Anh Thuy NGUYEN DANG ,  Melissa Siu-Lan MUN ,  Kristen Leigh DOUGLAS ,  Robert David KISS

IPC: C12P21/00 ,  C07K16/28 ,  C12N5/00

Abstract: The presently disclosed subject matter relates to cell culture media and cell culture strategies for modulating the glycosylation pattern, e.g., fucosylation and/or galactosylation pattern, of a glycoprotein of interest, e.g., an antibody, as well as cell culture and glycoprotein compositions prepared using such media and/or strategies.