公开(公告)号：US20210403953A1

公开(公告)日：2021-12-30

申请号：US17351451

申请日：2021-06-18

Applicant: GENENTECH, INC.

Inventor： Amy SHEN ,  Shahram MISAGHI

IPC: C12N15/90 ,  C12N15/85

Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins wherein those TI host cells have been subjected to supertransfection resulting in the random integration (RI) of exogenous nucleic acids encodes into their genome, as well as methods of producing and using said supertransfected TI host cells.

公开(公告)号：US20210309989A1

公开(公告)日：2021-10-07

申请号：US17213611

申请日：2021-03-26

Applicant: GENENTECH, INC.

Inventor： Michael Wilson LAIRD ,  Shahram MISAGHI ,  Amy SHEN ,  Anthony TOMLINSON ,  Inn Huam YUK

IPC: C12N15/10 ,  C12N5/071

Abstract: The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.

公开(公告)号：US20190040435A1

公开(公告)日：2019-02-07

申请号：US15994950

申请日：2018-05-31

Applicant: Genentech, Inc.

Inventor： Meixia ZHOU ,  Bradley Richard SNEDECOR ,  Chi Kin Domingos NG ,  Amy SHEN

IPC: C12P21/00 ,  C12N15/113 ,  C12N1/38 ,  C12N15/85 ,  C12N15/11

CPC classification number: C12P21/00 ,  C12N1/38 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/85 ,  C12N2310/14 ,  C12N2320/00 ,  C12Y101/01027 ,  C12Y102/04001

Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

公开(公告)号：US20230374497A1

公开(公告)日：2023-11-23

申请号：US18176062

申请日：2023-02-28

Applicant: GENENTECH, INC. ,  HOFFMANN-LA ROCHE INC.

Inventor： Amy SHEN ,  Inn Huam Yuk ,  Peggy Wai No Ko ,  Shahram Misaghi ,  Simon Thomas Auslaender ,  Midori Greenwood-Goodwin ,  Michael Wilson Laird ,  Benedikt Alois Claudius Oswald

IPC: C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90 ,  C12N5/00

CPC classification number: C12N15/1082 ,  C12N15/11 ,  C12N9/22 ,  C12N15/907 ,  C12N5/00 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure relates to modified mammalian cells having reduced or eliminated expression of certain cellular proteins, CRISPR/Cas9 multiplex knockout strategies for making such cells, and methods of using such cells, e.g., in the context of cell-based therapy or as host cells in the production of a product of interest.

公开(公告)号：US20170191102A1

公开(公告)日：2017-07-06

申请号：US15268424

申请日：2016-09-16

Applicant: Genentech, Inc.

Inventor： Meixia ZHOU ,  Bradley Richard SNEDECOR ,  Chi Kin Domingos NG ,  Amy SHEN

IPC: C12P21/00 ,  C12N15/113

CPC classification number: C12P21/00 ,  C12N1/38 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/85 ,  C12N2310/14 ,  C12N2320/00 ,  C12Y101/01027 ,  C12Y102/04001

Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

公开(公告)号：US20250101411A1

公开(公告)日：2025-03-27

申请号：US18823189

申请日：2024-09-03

Applicant: Genentech, Inc.

Inventor： Michael Wilson LAIRD ,  Shahram MISAGHI ,  Amy SHEN ,  Anthony TOMLINSON ,  Inn Huam YUK

IPC: C12N15/10 ,  C12N5/071

Abstract: The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.

公开(公告)号：US20210403943A1

公开(公告)日：2021-12-30

申请号：US17355605

申请日：2021-06-23

Applicant: GENENTECH, INC.

Inventor： Amy SHEN ,  Shahram MISAGHI ,  Cynthia Young LAM

IPC: C12N15/85 ,  C12P21/00 ,  C12N5/10 ,  C07K16/00

Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins wherein those TI host cells have been subjected to supertransfection resulting in the random integration (RI) of exogenous nucleic acids encodes into their genome, as well as methods of producing and using said supertransfected TI host cells.

公开(公告)号：US20200377919A1

公开(公告)日：2020-12-03

申请号：US16888344

申请日：2020-05-29

Applicant: Genentech, Inc.

Inventor： Meixia ZHOU ,  Bradley Richard SNEDECOR ,  Chi Kin Domingos NG ,  Amy SHEN

IPC: C12P21/00 ,  C12N15/113 ,  C12N1/38 ,  C12N15/11 ,  C12N15/85

Abstract: The present invention provides ethods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.