公开(公告)号：US20240240156A1

公开(公告)日：2024-07-18

申请号：US18510297

申请日：2023-11-15

Applicant: Genentech, Inc.

Inventor： Amy Shen ,  Gavin Christian Barnard ,  Shuet Ming Yip

IPC: C12N5/071 ,  C07K16/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/85

CPC classification number: C12N5/0682 ,  C07K16/00 ,  C12N9/22 ,  C12N15/111 ,  C12N15/85 ,  C07K2317/622 ,  C12N2310/20 ,  C12N2840/002

Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins where the TI host cells are also subjected to transposon-mediated genomic integration of one or more exogenous nucleic acids, as well as methods of producing and using said combined transposon-mediated genomic integration and TI host cells.

公开(公告)号：US20220162295A1

公开(公告)日：2022-05-26

申请号：US17383316

申请日：2021-07-22

Applicant: Hoffmann-La Roche Inc. ,  Genentech, Inc.

Inventor： Styliani Tournaviti ,  Shan-Hua Chung ,  Harald Duerr ,  Claudia Ferrara Koller ,  Amy Shen ,  Shahram Misaghi ,  Shuet Ming Yip

IPC: C07K16/22 ,  C07K16/24

Abstract: Herein is reported a method for producing an antibody-multimer-fusion polypeptide comprising (a) an antibody heavy chain and an antibody light chain, and (b) a first fusion polypeptide comprising in N- to C-terminal direction a first part of a non-antibody multimeric polypeptide, an antibody heavy chain CH1 domain or an antibody light chain constant domain, an antibody hinge region, an antibody heavy chain CH2 domain and an antibody heavy chain CH3 domain, and a second fusion polypeptide comprising in N- to C-terminal direction the second part of the non-antibody multimeric polypeptide and an antibody light chain constant domain if the first polypeptide comprises an antibody heavy chain CH1 domain or an antibody heavy chain CH1 domain if the first polypeptide comprises an antibody light chain constant domain, wherein (i) the antibody heavy chain of (a) and the first fusion polypeptide of (b), (ii) the antibody heavy chain of (a) and the antibody light chain of (a), and (iii) the first fusion polypeptide of (b) and the second fusion polypeptide of (b) are each independently of each other covalently linked to each other by at least one disulfide bond, characterized in that the antibody-multimer-fusion is expressed by a recombinant mammalian cell obtained by transfecting a mammalian cell with the expression cassettes for the antibody heavy chain, the antibody light chain, the first fusion polypeptide and the second fusion polypeptide at a stoichiometric ratio of 1:1:2:1.