公开(公告)号：US10759866B2

公开(公告)日：2020-09-01

申请号：US16240592

申请日：2019-01-04

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  C07K1/14 ,  A61K39/395

Abstract: Provide herein are methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US12145998B2

公开(公告)日：2024-11-19

申请号：US17809098

申请日：2022-06-27

Applicant: Genentech, Inc.

Inventor： Lynn A. Gennaro ,  Yung-Hsiang Kao ,  Yonghua Zhang

IPC: A61K39/395 ,  C07K16/30 ,  C07K16/32 ,  G01N33/68 ,  A61K39/00

Abstract: The present application discloses variants of Pertuzumab. In particular, it discloses: an unpaired cysteine variant comprising Cys23/Cys88 unpaired cysteines in one or both variable light domains of Pertuzumab, an afucosylated variant of Pertuzumab, a low-molecular-weight-species (LMWS) of Pertuzumab, and a high-molecular-weight-species (HMWS) or Pertuzumab. The application further discloses the isolated variants, compositions, pharmaceutical compositions, and articles of manufacture comprising the variants, as well as methods of making and characterizing the variants and compositions thereof.

公开(公告)号：US12098211B2

公开(公告)日：2024-09-24

申请号：US18194471

申请日：2023-03-31

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  A61P5/00 ,  A61P37/02 ,  C07K1/14 ,  C07K1/22 ,  C07K14/00 ,  C07K16/32 ,  C12N1/38 ,  C12N5/00

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11999791B2

公开(公告)日：2024-06-04

申请号：US18310448

申请日：2023-05-01

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  A61P5/00 ,  A61P37/02 ,  C07K1/14 ,  C07K1/22 ,  C07K14/00 ,  C07K16/32 ,  C12N1/38 ,  C12N5/00

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11987637B1

公开(公告)日：2024-05-21

申请号：US18410876

申请日：2024-01-11

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US10808037B1

公开(公告)日：2020-10-20

申请号：US16847309

申请日：2020-04-13

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  C07K1/14 ,  A61K39/395

Abstract: Provided herein are methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11987638B1

公开(公告)日：2024-05-21

申请号：US18410949

申请日：2024-01-11

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11976127B1

公开(公告)日：2024-05-07

申请号：US18304286

申请日：2023-04-20

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20240132612A1

公开(公告)日：2024-04-25

申请号：US18304286

申请日：2023-04-20

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20230047103A1

公开(公告)日：2023-02-16

申请号：US17809098

申请日：2022-06-27

Applicant: Genentech, Inc.

Inventor： Lynn A. Gennaro ,  Yung-Hsiang Kao ,  Yonghua Zhang

IPC: C07K16/30 ,  G01N33/68 ,  C07K16/32

Abstract: The present application discloses variants of Pertuzumab. In particular, it discloses: an unpaired cysteine variant comprising Cys23/Cys88 unpaired cysteines in one or both variable light domains of Pertuzumab, an afucosylated variant of Pertuzumab, a low-molecular-weight-species (LMWS) of Pertuzumab, and a high-molecular-weight-species (HMWS) or Pertuzumab. The application further discloses the isolated variants, compositions, pharmaceutical compositions, and articles of manufacture comprising the variants, as well as methods of making and characterizing the variants and compositions thereof.