公开(公告)号：US20170267983A1

公开(公告)日：2017-09-21

申请号：US15444020

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Arkadiusz Bibillo ,  Preethi Sarvabhowman ,  Dhruti Vasudev Dalal ,  Ilya Lederman ,  Eileen Thai

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Y207/07007

Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.

公开(公告)号：US10308918B2

公开(公告)日：2019-06-04

申请号：US15012317

申请日：2016-02-01

Applicant: Genia Technologies, Inc. ,  Roche Molecular Systems, Inc.

Inventor： Aruna Ayer ,  Arkadiusz Bibillo ,  Cleoma Renetta Arnold ,  Charles Wayan Schwab ,  Eileen Thai ,  Ilya Lederman ,  Colin Alexander McGaw ,  Tyler O'Brien Shultz ,  Barbara Eckert ,  Shawn Suko ,  Mara Boenitz-Dulat ,  Bigna Woersdoerfer ,  David Daniel Wunderlich

IPC: C12N9/12

Abstract: Described herein are variant pol6 polymerase having at least having at least two substitutions, wherein at least one of the substitutions is at position S366 of SEQ ID NO: 2. The disclosed polymerases may be coupled to alpha-hemolysin nanopores, and may be used in nanopore-based sequencing apparatuses.

公开(公告)号：US10266813B2

公开(公告)日：2019-04-23

申请号：US15444020

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Arkadiusz Bibillo ,  Preethi Sarvabhowman ,  Dhruti Vasudev Dalal ,  Ilya Lederman ,  Eileen Thai

IPC: C12N9/12 ,  C12N15/00 ,  C07H21/04

Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.

公开(公告)号：US20190249154A1

公开(公告)日：2019-08-15

申请号：US16398089

申请日：2019-04-29

Applicant: Roche Molecular Systems, Inc. ,  Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Cleoma Renetta Arnold ,  Arkadiusz Bibillo ,  Mara Boenitz-Dulat ,  Barbara Eckert ,  Ilya Lederman ,  Colin Alexander McGaw ,  Charles Wayan Schwab ,  Tyler O'Brien Shultz ,  Shawn Suko ,  Eileen Thai ,  Bigna Woersdoerfer ,  David Daniel Wunderlich

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C07K2319/21 ,  C12Y207/07007

Abstract: Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, 1644, T647, I648, T651, 1652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.