公开(公告)号：US20190249154A1

公开(公告)日：2019-08-15

申请号：US16398089

申请日：2019-04-29

Applicant: Roche Molecular Systems, Inc. ,  Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Cleoma Renetta Arnold ,  Arkadiusz Bibillo ,  Mara Boenitz-Dulat ,  Barbara Eckert ,  Ilya Lederman ,  Colin Alexander McGaw ,  Charles Wayan Schwab ,  Tyler O'Brien Shultz ,  Shawn Suko ,  Eileen Thai ,  Bigna Woersdoerfer ,  David Daniel Wunderlich

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C07K2319/21 ,  C12Y207/07007

Abstract: Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, 1644, T647, I648, T651, 1652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.

公开(公告)号：US20170268052A1

公开(公告)日：2017-09-21

申请号：US15444042

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Preethi Sarvabhowman ,  Charles Schwab

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6806 ,  C12Y207/07007 ,  C12Q2565/631

Abstract: The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.

公开(公告)号：US20170267983A1

公开(公告)日：2017-09-21

申请号：US15444020

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Arkadiusz Bibillo ,  Preethi Sarvabhowman ,  Dhruti Vasudev Dalal ,  Ilya Lederman ,  Eileen Thai

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Y207/07007

Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.

公开(公告)号：US10308918B2

公开(公告)日：2019-06-04

申请号：US15012317

申请日：2016-02-01

Applicant: Genia Technologies, Inc. ,  Roche Molecular Systems, Inc.

Inventor： Aruna Ayer ,  Arkadiusz Bibillo ,  Cleoma Renetta Arnold ,  Charles Wayan Schwab ,  Eileen Thai ,  Ilya Lederman ,  Colin Alexander McGaw ,  Tyler O'Brien Shultz ,  Barbara Eckert ,  Shawn Suko ,  Mara Boenitz-Dulat ,  Bigna Woersdoerfer ,  David Daniel Wunderlich

IPC: C12N9/12

Abstract: Described herein are variant pol6 polymerase having at least having at least two substitutions, wherein at least one of the substitutions is at position S366 of SEQ ID NO: 2. The disclosed polymerases may be coupled to alpha-hemolysin nanopores, and may be used in nanopore-based sequencing apparatuses.

公开(公告)号：US10266813B2

公开(公告)日：2019-04-23

申请号：US15444020

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Arkadiusz Bibillo ,  Preethi Sarvabhowman ,  Dhruti Vasudev Dalal ,  Ilya Lederman ,  Eileen Thai

IPC: C12N9/12 ,  C12N15/00 ,  C07H21/04

Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.

公开(公告)号：US20180245147A1

公开(公告)日：2018-08-30

申请号：US15443964

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Preethi Sarvabhowman ,  Dhruti Vasudev Dalal

IPC: C12Q1/68 ,  C12N9/12 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  C12Q2521/101 ,  C12Q2565/631

Abstract: The present of disclosure provides variant Pol6 polymerase polypeptide, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result n increased processivity relative to the parental Pol6 polypeptides from which they are derived.