公开(公告)号：US20240150798A1

公开(公告)日：2024-05-09

申请号：US18482834

申请日：2023-10-06

Applicant: Genomatica, Inc.

Inventor： Benjamin ADELSTEIN ,  Robin E. OSTERHOUT ,  Mark J. BURK

IPC: C12P7/62 ,  A23L33/135 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07C69/675 ,  C12N1/00 ,  C12N1/14 ,  C12N1/20 ,  C12N9/18 ,  C12N15/52

CPC classification number: C12P7/62 ,  A23L33/135 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07C69/675 ,  C12N1/00 ,  C12N1/145 ,  C12N1/205 ,  C12N9/18 ,  C12N15/52 ,  C12Y101/01035 ,  C12Y101/011 ,  C12Y301/01067 ,  C12Y301/08001 ,  C07B2200/05 ,  C12R2001/00 ,  Y02P20/10

Abstract: Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.

公开(公告)号：US20160108442A1

公开(公告)日：2016-04-21

申请号：US14893510

申请日：2014-05-23

Applicant: Genomatica, Inc.

Inventor： Benjamin ADELSTEIN ,  Robin E. OSTERHOUT ,  Mark J. BURK

IPC: C12P7/62 ,  C07C69/675 ,  C12N15/52 ,  C12R1/01 ,  C12R1/645

CPC classification number: C12P7/62 ,  A23L33/135 ,  A23V2002/00 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07B2200/05 ,  C07C69/675 ,  C12N9/18 ,  C12N15/52 ,  C12P7/42 ,  C12R1/01 ,  C12R1/645 ,  C12Y101/01035 ,  C12Y101/011 ,  C12Y301/01067 ,  C12Y301/08001 ,  Y02P20/127

Abstract: Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.

Abstract translation: 本文提供了具有（3R） - （3R） - 羟基丁酸生产途径的非天然存在的微生物生物，其中生物体还可包含（R）-1,3-丁二醇途径，（3R） - 羟基丁酸酯 途径，（3R） - 羟基丁酰辅酶A途径，乙酰乙酸途径，乙酰乙酰辅酶A途径，（3R） - 羟基丁基ACP途径或乙酰乙酰-ACP途径。 另外提供了使用微生物生物体（3R） - 羟基丁酸（3R） - 羟基丁酸生产和分离（3R） - 羟基丁酸酯的方法和方法，以及具有（3R） - 羟基丁基（3R） - 羟基丁酸酯的各种组合物。 还提供了使用本发明的微生物生物体产生的（3R） - 羟基丁酸（3R） - 羟基丁酸酯来治疗或预防疾病，病症或病症的方法。