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    Preparation of an esterase
    5.
    发明授权
    Preparation of an esterase 有权
    酯酶的制备

    公开(公告)号：US09309503B2

    公开(公告)日：2016-04-12

    申请号：US12667414

    申请日：2008-07-04

    申请人： Martin Kietzmann ,  Helmut Schwab ,  Harald Pichler ,  Mirela Ivancic ,  Oliver May ,  Rudolf Gijsbertus Marie Luiten

    发明人： Martin Kietzmann ,  Helmut Schwab ,  Harald Pichler ,  Mirela Ivancic ,  Oliver May ,  Rudolf Gijsbertus Marie Luiten

    IPC分类号： C12P21/02 ,  C12N9/18 ,  C12N15/70

    CPC分类号： C12N9/18 ,  C12N15/70 ,  C12Y301/01001

    摘要： The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.

    摘要翻译： 本发明涉及重组大肠杆菌菌株，其特征在于生物体含有编码具有酯酶活性的蛋白质的基因。 这种大肠杆菌适用于制备具有酯酶活性的蛋白质，从而在不与GroEL和/或GroES共同表达质粒的情况下进行表达。 谷胱甘肽还原酶和/或硫氧还蛋白还原酶的表达可以通过突变来消除。 此外，生物体在富氧条件下生长的能力已经通过突变恢复。 具有酯酶活性的蛋白质基因的表达与编码热休克分子伴侣蛋白的另外的基因的表达无关。

    PREPARATION OF AN ESTERASE
    6.
    发明申请
    PREPARATION OF AN ESTERASE 有权
    蛋白酶的制备

    公开(公告)号：US20110177553A1

    公开(公告)日：2011-07-21

    申请号：US12667414

    申请日：2008-07-04

    申请人： Martin Kietzmann ,  Helmut Schwab ,  Harald Pichler ,  Mirela Ivancic ,  Oliver May ,  Rudolf Gijsbertus Marie Luiten

    发明人： Martin Kietzmann ,  Helmut Schwab ,  Harald Pichler ,  Mirela Ivancic ,  Oliver May ,  Rudolf Gijsbertus Marie Luiten

    IPC分类号： C12P21/02 ,  C12N1/21 ,  C12N15/55 ,  C12N15/70 ,  C12P7/40

    CPC分类号： C12N9/18 ,  C12N15/70 ,  C12Y301/01001

    摘要： The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.

    摘要翻译： 本发明涉及重组大肠杆菌菌株，其特征在于生物体含有编码具有酯酶活性的蛋白质的基因。 这种大肠杆菌适用于制备具有酯酶活性的蛋白质，从而在不与GroEL和/或GroES共同表达质粒的情况下进行表达。 谷胱甘肽还原酶和/或硫氧还蛋白还原酶的表达可以通过突变来消除。 此外，生物体在富氧条件下生长的能力已经通过突变恢复。 具有酯酶活性的蛋白质基因的表达与编码热休克分子伴侣蛋白的另外的基因的表达无关。

    Pig liver esterases
    7.
    发明授权
    Pig liver esterases 有权
    猪肝酯酶

    公开(公告)号：US08921080B2

    公开(公告)日：2014-12-30

    申请号：US13265178

    申请日：2010-04-26

    申请人： Martin Kietzmann ,  Harald Pichler ,  Helmut Schwab ,  Amin El-Heliebi ,  Christine Winkler ,  Andreas Braun

    发明人： Martin Kietzmann ,  Harald Pichler ,  Helmut Schwab ,  Amin El-Heliebi ,  Christine Winkler ,  Andreas Braun

    IPC分类号： C12N9/18

    CPC分类号： C12N9/18

    摘要： The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in E. coli relative to the concentration of the fraction of the polypeptide without the mutation being present as an active and soluble protein in cleared lysate of the polypeptide without the one or more deletion or substitution expressed in E. coli under the same conditions. The invention also relates to nucleic acid encoding the polypeptides according to the invention, and the use of the polypeptides.

    摘要翻译： 本发明涉及具有酯酶活性的分离多肽，其包含SEQ ID NO：2,4,6,8,10,12或14中任一项所示的氨基酸序列或其同源物，其包含氨基酸取代或缺失的一个 或更多的氨基酸，如所述SEQ ID NO所示，并且产生突变体多肽的浓度增加，所述突变体多肽作为活性和可溶性蛋白质存在于大肠杆菌中表达的突变多肽的澄清裂解物中，相对于 不存在突变的多肽的级分的浓度作为活性和可溶性蛋白质存在于多肽的澄清裂解物中，而在相同条件下在大肠杆菌中表达的一个或多个缺失或取代。 本发明还涉及编码根据本发明的多肽的核酸和多肽的用途。

    PIG LIVER ESTERASES
    8.
    发明申请
    PIG LIVER ESTERASES 有权
    猪肝病

    公开(公告)号：US20120100582A1

    公开(公告)日：2012-04-26

    申请号：US13265178

    申请日：2010-04-26

    申请人： Martin Kietzmann ,  Harald Pichler ,  Helmut Schwab ,  Amin El-Heliebi ,  Christine Winkler ,  Andreas Braun

    发明人： Martin Kietzmann ,  Harald Pichler ,  Helmut Schwab ,  Amin El-Heliebi ,  Christine Winkler ,  Andreas Braun

    IPC分类号： C12P7/62 ,  C12P7/02 ,  C12P7/40 ,  C12N9/18 ,  C12N15/55

    CPC分类号： C12N9/18

    摘要： The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in E. coli relative to the concentration of the fraction of the polypeptide without the mutation being present as an active and soluble protein in cleared lysate of the polypeptide without the one or more deletion or substitution expressed in E. coli under the same conditions. The invention also relates to nucleic acid encoding the polypeptides according to the invention, and the use of the polypeptides.

    摘要翻译： 本发明涉及具有酯酶活性的分离多肽，其包含SEQ ID NO：2,4,6,8,10,12或14中任一项所示的氨基酸序列或其同源物，其包含氨基酸取代或缺失的一个 或更多的氨基酸，如所述SEQ ID NO所示，并且产生突变体多肽的浓度增加，所述突变体多肽作为活性和可溶性蛋白质存在于大肠杆菌中表达的突变多肽的澄清裂解物中，相对于 未突变的多肽的级分的浓度作为活性和可溶性蛋白质存在于多肽的澄清裂解物中，而在相同条件下在大肠杆菌中不表达一个或多个缺失或取代。 本发明还涉及编码根据本发明的多肽的核酸和多肽的用途。

    Drop-safety mechanism for a firearm
    9.
    发明授权
    Drop-safety mechanism for a firearm 有权
    枪支的落锤安全机制

    公开(公告)号：US08677666B2

    公开(公告)日：2014-03-25

    申请号：US13378900

    申请日：2010-05-06

    申请人： Harald Pichler

    发明人： Harald Pichler

    IPC分类号： F41A17/00

    CPC分类号： F41A17/46

    摘要： The invention relates to a drop-safety mechanism for a firearm having a pivot-mounted, trigger, said trigger being designed as an arm of a double lever, the other arm of which supports a counterweight that can be displaced between a rest position and a functional position. The counterweight is resiliently pre-tensioned in the rest position and strikes on a part of the firearm in the functional position to block the trigger from unintended firing.

    摘要翻译： 本发明涉及一种具有枢转安装的触发器的枪支的液滴安全机构，所述触发器被设计为双杠杆的臂，另一个臂支撑一个可在静止位置和静止位置之间移动的配重 功能位置。 配重弹性地预紧在休息位置，并且在功能位置上的枪支的一部分上撞击，以阻止触发器意外着火。

    Cocking And Uncocking Mechanism For A Firearm
    10.
    发明申请
    Cocking And Uncocking Mechanism For A Firearm 有权
    枪支的。ing机制

    公开(公告)号：US20120090450A1

    公开(公告)日：2012-04-19

    申请号：US13378876

    申请日：2010-05-06

    申请人： Harald Pichler

    发明人： Harald Pichler

    IPC分类号： F41A5/00 ,  F41A17/64 ,  F41A19/34

    CPC分类号： F41A19/31 ,  F41A19/12 ,  F41A19/34 ,  F41A19/41

    摘要： The invention relates to a cocking and uncocking mechanism for a firearm, comprising a spring-loaded firing pin which, when in the cocked position, engages on a catch that can be released to fire, said catch being displaceable by an actuator element acting upon the catch via a link motion the control cam of which maintains the force to be applied to the actuator element to displace the catch to the cocked position substantially constant at least in the center region of the actuation path.

    摘要翻译： 本发明涉及一种用于枪支的起动和起跳机构，包括弹簧加载的击发销，当处于被击发位置时，该弹簧加载的击发销接合在可被释放的火焰上的卡扣上，所述卡扣可通过作用在该弹簧上的致动器元件 通过其连杆运动来抓住其控制凸轮，其保持施加到致动器元件的力，以将至少在致动路径的中心区域中的锁定位置基本上恒定。