Methods And Kits For In Situ Measurement of Enzyme Activity and Amount Using Single Measurement System
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    发明申请
    Methods And Kits For In Situ Measurement of Enzyme Activity and Amount Using Single Measurement System 审中-公开
    使用单一测量系统原位测量酶活性和量的方法和试剂盒

    公开(公告)号:US20090286223A1

    公开(公告)日:2009-11-19

    申请号:US12302418

    申请日:2006-05-30

    IPC分类号: C12Q1/70 G01N33/53

    摘要: Disclosed are methods and kits for measuring in situ the activity and amount of an enzyme in a sample using a single measurement system. The method includes the steps of: (a) contacting a sample to a capturing agent having the capacity to bind to the enzyme to be analyzed and immobilized on a solid matrix; (b) measuring the activity of the enzyme captured by the capturing agent; (c) contacting a detection antibody specific to the enzyme captured by the capturing agent; and (d) detecting an antigen-antibody complex formed in the step (c). There is no need for separate measurement system to measure the activity and amount of an enzyme, as well as the above methods and kits permit to measure the precise activity and amount of an enzyme simultaneously because the measurement of enzyme activity and amount is carried out in a single and same measurement system as to the same sample in almost simultaneous manner.

    摘要翻译: 公开了用于使用单个测量系统原位测量样品中的酶的活性和量的方法和试剂盒。 该方法包括以下步骤:(a)将样品与能够结合待分析和固定在固体基质上的酶的捕获剂接触; (b)测量由捕获剂捕获的酶的活性; (c)使由捕获剂捕获的酶特异的检测抗体接触; 和(d)检测步骤(c)中形成的抗原 - 抗体复合物。 不需要单独的测量系统来测量酶的活性和量,以及上述方法和试剂盒允许同时测量酶的精确活性和量,因为酶活性和量的测量在 以同样的方式对相同样品进行单一和相同的测量系统。