公开(公告)号：US20090036665A1

公开(公告)日：2009-02-05

申请号：US12172086

申请日：2008-07-11

申请人： Gonzalo Domingo ,  Bernhard Weigl ,  Paul Labarre ,  Jay Gerlach

发明人： Gonzalo Domingo ,  Bernhard Weigl ,  Paul Labarre ,  Jay Gerlach

IPC分类号： C07H21/00 ,  B01L11/00

CPC分类号： C12Q1/6806 ,  B01L3/502 ,  B01L7/00 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/1855 ,  B01L2300/1877 ,  B01L2400/0478 ,  B01L2400/0622 ,  B01L2400/0644

摘要： A low-cost, non-instrumented, easy-to-use disposable platform for extraction, stabilization, and preservation of viral RNA in specimens at the point of collection is described. The system may use chemical heating. The platform performs the following steps: specimen lysis, RNA extraction, and RNA stabilization in a modular approach. This modular approach confers versatility to the product for application to multiple targets such as avian flu, and HIV, specimens such as blood, nasal swabs, and downstream applications such as PCR or transcription-mediated amplification. The technology described is a point-of-care specimen-processing platform generically applicable to both emerging point-of-care and central-facility molecular diagnostic tests, as well as to surveillance applications.

摘要翻译： 描述了一种低成本，非仪器化，易于使用的一次性平台，用于在收集点提取，稳定和保存样品中的病毒RNA。 该系统可以使用化学加热。 该平台执行以下步骤：模块化方法中的样品裂解，RNA提取和RNA稳定。 这种模块化方法赋予产品多种多样性，适用于多种靶点，如禽流感和HIV，标本如血液，鼻拭子和下游应用如PCR或转录介导的扩增。 所描述的技术是通常适用于新兴护理点和中心设施分子诊断测试以及监视应用的点护理样本处理平台。