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公开(公告)号：US08921279B2
公开(公告)日：2014-12-30
申请号：US12666618
申请日：2008-06-26
CPC分类号： C07K16/005 , C07K16/32 , C07K2317/52 , C07K2317/569 , C07K2318/10 , C40B30/04 , G01N33/6857 , G01N2333/70535
摘要： The present invention relates to a method of preparing a genetic package displaying oligomers of modular antibody domains binding to a target and to a scaffold ligand as well as to vectors and libraries of genetic packages produced thereby. The invention further relates to methods of selecting suitable linker sequences for use in such oligomer display.
摘要翻译： 本发明涉及制备显示结合靶标和支架配体的模块抗体结构域的寡聚体以及由此产生的遗传包装的载体和文库的遗传包的方法。 本发明还涉及选择用于这种低聚物显示的合适的接头序列的方法。
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公开(公告)号：US20100184615A1
公开(公告)日：2010-07-22
申请号：US12666618
申请日：2008-06-26
CPC分类号： C07K16/005 , C07K16/32 , C07K2317/52 , C07K2317/569 , C07K2318/10 , C40B30/04 , G01N33/6857 , G01N2333/70535
摘要： The present invention relates to a method of preparing a genetic package displaying oligomers of modular antibody domains binding to a target and to a scaffold ligand as well as to vectors and libraries of genetic packages produced thereby. The invention further relates to methods of selecting suitable linker sequences for use in such oligomer display.
摘要翻译： 本发明涉及制备显示结合靶标和支架配体的模块抗体结构域的寡聚体以及由此产生的遗传包装的载体和文库的遗传包的方法。 本发明还涉及选择用于这种低聚物显示的合适的接头序列的方法。
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公开(公告)号：US09133274B2
公开(公告)日：2015-09-15
申请号：US12307569
申请日：2007-07-05
CPC分类号： C12N15/1037 , C07K16/00 , C07K16/005 , C07K16/1045 , C07K16/1063 , C07K16/16 , C07K16/18 , C07K16/241 , C07K16/283 , C07K16/32 , C07K16/40 , C07K2317/21 , C07K2317/22 , C07K2317/55 , C07K2317/622
摘要： A method for engineering an immunoglobulin having a variable domain and at least one modification in at least two structural loops of the immunoglobulin and determining the binding of the immunoglobulin to an epitope of an antigen, where the unmodified immunoglobulin does not significantly bind to said epitope, comprising the steps of providing a nucleic acid encoding an immunoglobulin having at least two structural loops, modifying at least one nucleotide residue of each of the structural loops, transferring the modified nucleic acid in an expression system, expressing the modified immunoglobulin, contacting the expressed modified immunoglobulin with an epitope, and determining whether the modified immunoglobulin binds to the epitope.
摘要翻译： 一种用于在免疫球蛋白的至少两个结构环中设计具有可变结构域和至少一个修饰的免疫球蛋白的方法，并确定免疫球蛋白与抗原表位的结合，其中未修饰的免疫球蛋白不显着结合所述表位， 包括以下步骤：提供编码具有至少两个结构环的免疫球蛋白的核酸，修饰每个结构环的至少一个核苷酸残基，在表达系统中转移修饰的核酸，表达经修饰的免疫球蛋白， 免疫球蛋白，并确定修饰的免疫球蛋白是否结合表位。
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公开(公告)号：US09856311B2
公开(公告)日：2018-01-02
申请号：US13149871
申请日：2011-05-31
CPC分类号： C07K16/00 , C07K16/28 , C07K16/40 , C07K19/00 , C07K2317/21 , C07K2317/31 , C07K2317/52 , C07K2317/522 , C07K2317/526 , C07K2317/55 , C07K2318/20 , C07K2319/30 , C40B40/10
摘要： Libraries of immunoglobulins which each have one or more amino acid modifications in at least one structural loop region of such immunoglobulins, where the modified loop region specifically binds to an epitope of an antigen to which an unmodified immunoglobulin does not significantly bind.
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公开(公告)号：US20100048877A1
公开(公告)日：2010-02-25
申请号：US12307578
申请日：2007-06-26
CPC分类号： C07K16/468 , C07K16/005 , C07K16/28 , C07K16/2887 , C07K2317/52
摘要： The present invention provides a multivalent immunoglobulin or part thereof binding specifically to at least two cell surface molecules of a single cell with at least one modification in at least one structural loop region of said immunoglobulin determining binding to an epitope of said cell surface molecules wherein the unmodified immunoglobulin does not significantly bind to said epitope, its use and methods for producing it.
摘要翻译： 本发明提供了多价免疫球蛋白或其部分特异性结合至单个细胞的至少两个细胞表面分子，所述免疫球蛋白在所述免疫球蛋白的至少一个结构环区具有至少一个修饰，确定与所述细胞表面分子的表位的结合，其中 未修饰的免疫球蛋白不显着结合所述表位，其用途及其生产方法。
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6.发明授权Synthetic immunoglobulin domains with binding properties engineered in regions of the molecule different from the complementarity determining regions 有权具有在不同于互补决定区的分子区域中工程化的结合特性的合成免疫球蛋白结构域公开(公告)号：US09045528B2
公开(公告)日：2015-06-02
申请号：US13151195
申请日：2011-06-01
CPC分类号： C07K16/00 , C07K16/28 , C07K16/40 , C07K19/00 , C07K2317/21 , C07K2317/31 , C07K2317/52 , C07K2317/522 , C07K2317/526 , C07K2317/55 , C07K2318/20 , C07K2319/30 , C40B40/10
摘要： A method of engineering immunoglobulins which each have one or more amino acid modifications in at least one structural loop region of such immunoglobulins, where the modified loop region specifically binds to an epitope of an antigen to which an unmodified immunoglobulin does not significantly bind.
摘要翻译： 一种设计免疫球蛋白的方法，其各自在这种免疫球蛋白的至少一个结构环区域中具有一个或多个氨基酸修饰，其中修饰的环区特异性结合未修饰的免疫球蛋白不显着结合的抗原的表位。
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公开(公告)号：US20120094874A1
公开(公告)日：2012-04-19
申请号：US13377817
申请日：2010-07-01
CPC分类号： C07K16/00 , C07K2317/52 , C07K2318/20
摘要： The invention refers to a multidomain modular antibody comprising at least one constant antibody domain, which is mutated to form an artificial disulfide bridge by introducing at least one Cys residue into the amino acid sequence through mutagenesis of said constant domain to obtain an intra-domain or inter-domain disulfide bridge within the framework region, libraries based on such antibodies and methods of producing.
摘要翻译： 本发明涉及包含至少一个恒定抗体结构域的多结构域模块抗体，其通过所述恒定结构域的突变将至少一个Cys残基引入氨基酸序列而被突变以形成人造二硫键，以获得一个域内或 在框架区内的域间二硫键，基于这种抗体的文库和生产方法。
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8.发明申请SYNTHETIC IMMUNOGLOBULIN DOMAINS WITH BINDING PROPERTIES ENGINEERED IN REGIONS OF THE MOLECULE DIFFERENT FROM THE COMPLEMENTARITY DETERMINING REGIONS 审中-公开具有结合性质的合成免疫球蛋白结构域，其功能区域在完全相同的区域中分布不同公开(公告)号：US20090298195A1
公开(公告)日：2009-12-03
申请号：US11722517
申请日：2006-01-05
IPC分类号： G01N33/566 , G01N33/53 , G01N33/573 , C12Q1/70 , C07K16/00 , C07K16/30 , C12N9/96 , C40B40/10
CPC分类号： C07K16/00 , C07K16/28 , C07K16/40 , C07K19/00 , C07K2317/21 , C07K2317/31 , C07K2317/52 , C07K2317/522 , C07K2317/526 , C07K2317/55 , C07K2318/20 , C07K2319/30 , C40B40/10
摘要： Method for engineering an immunoglobulin comprising at least one modification in a structural loop region of said immunoglobulin and determining the binding of said immunoglobulin to an epitope of an antigen, wherein the unmodified immunoglobulin does not significantly bind to said epitope, comprising the steps of:—providing a nucleic acid encoding an immunoglobulin comprising at least one structural loop region,—modifying at least one nucleotide residue of at least one of said structural loop regions,—transferring said modified nucleic acid in an expression system,—expressing said modified immunoglobulin,—contacting the expressed modified immunoglobulin with an epitope, and—determining whether said modified immunoglobulin binds to said epitope, as well as modified immunoglobulins.
摘要翻译： 用于工程化免疫球蛋白的方法，其包含所述免疫球蛋白的结构环区中的至少一个修饰，并确定所述免疫球蛋白与抗原表位的结合，其中所述未修饰的免疫球蛋白不显着结合所述表位，包括以下步骤： 提供编码包含至少一个结构环区的免疫球蛋白的核酸，修饰至少一个所述结构环区的至少一个核苷酸残基， - 在表达系统中转移所述修饰的核酸， - 表达所述修饰的免疫球蛋白， - 使表达的修饰的免疫球蛋白与表位接触，以及 - 确定所述修饰的免疫球蛋白是否结合所述表位，以及修饰的免疫球蛋白。
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