公开(公告)号：US20100065500A1

公开(公告)日：2010-03-18

申请号：US12516553

申请日：2006-12-01

申请人： Guanghui Ma ,  Zhiguo Su ,  Weiqing Zhou ,  Qiang Wei

发明人： Guanghui Ma ,  Zhiguo Su ,  Weiqing Zhou ,  Qiang Wei

IPC分类号： C08F2/18 ,  C08F2/20 ,  B01J20/26 ,  B01J13/14 ,  C08J9/00

CPC分类号： C08F2/18 ,  B01J20/28004 ,  B01J20/28085 ,  B01J20/285 ,  B01J20/3064 ,  B01J31/063 ,  B01J2220/54 ,  Y10T428/2982

摘要： The invention provides a preparation method of super-macroporous polymeric microspheres and a product thereof. The method is characterized in that adding high content of surfactant into an oil phase containing a monomer and dispersing the oil phase containing the monomer and the surfactant into an aqueous phase. Super-macroporous microspheres are obtained by means of suspension polymerization. In the interior of the microspheres, 10-60% of the pores are distributed in a range of 500-900 nm. The microspheres have a particle diameter of 1-200 μm and a porosity of 30%-90%. The product is suitable for being used as a stationary phase matrix in liquid chromatography, a carrier for high performance catalysts and a high performance adsorbent, and especially, being used as a separation medium for separating biomacromolecules.

摘要翻译： 本发明提供超大孔聚合物微球及其制品的制备方法。 该方法的特征在于将高含量的表面活性剂加入到含有单体的油相中并将含有单体和表面活性剂的油相分散在水相中。 通过悬浮聚合获得超大孔微球。 在微球的内部，10-60％的孔分布在500-900nm的范围内。 微球的粒径为1-200μm，孔隙率为30％-90％。 该产品适用于液相色谱中的固定相基质，高性能催化剂载体和高性能吸附剂，尤其用作分离生物大分子的分离介质。

公开(公告)号：US08940172B2

公开(公告)日：2015-01-27

申请号：US12516553

申请日：2006-12-01

申请人： Guanghui Ma ,  Zhiguo Su ,  Weiqing Zhou ,  Qiang Wei

发明人： Guanghui Ma ,  Zhiguo Su ,  Weiqing Zhou ,  Qiang Wei

IPC分类号： B01J20/30 ,  B01J31/06 ,  C08F2/18 ,  B01J20/285 ,  B01J20/28

CPC分类号： C08F2/18 ,  B01J20/28004 ,  B01J20/28085 ,  B01J20/285 ,  B01J20/3064 ,  B01J31/063 ,  B01J2220/54 ,  Y10T428/2982

摘要： The invention provides a preparation method of super-macroporous polymeric microspheres and a product thereof. The method is characterized in that adding high content of surfactant into an oil phase containing a monomer and dispersing the oil phase containing the monomer and the surfactant into an aqueous phase. Super-macroporous microspheres are obtained by means of suspension polymerization. In the interior of the microspheres, 10-60% of the pores are distributed in a range of 500-900 nm. The microspheres have a particle diameter of 1-200 μm and a porosity of 30%-90%. The product is suitable for being used as a stationary phase matrix in liquid chromatography, a carrier for high performance catalysts and a high performance adsorbent, and especially, being used as a separation medium for separating biomacromolecules.

摘要翻译： 本发明提供超大孔聚合物微球及其制品的制备方法。 该方法的特征在于将高含量的表面活性剂加入到含有单体的油相中并将含有单体和表面活性剂的油相分散在水相中。 通过悬浮聚合获得超大孔微球。 在微球的内部，10-60％的孔分布在500-900nm的范围内。 微球的粒径为1-200μm，孔隙率为30％-90％。 该产品适用于液相色谱中的固定相基质，高性能催化剂载体和高性能吸附剂，尤其用作分离生物大分子的分离介质。

公开(公告)号：US20100143259A1

公开(公告)日：2010-06-10

申请号：US12441894

申请日：2007-09-13

申请人： Guanghui Ma ,  Zhiguo Su ,  Wei Wei ,  Lianyan Wang ,  Qiang Wei

发明人： Guanghui Ma ,  Zhiguo Su ,  Wei Wei ,  Lianyan Wang ,  Qiang Wei

IPC分类号： A61K49/00 ,  C07H1/00 ,  C12Q1/02

CPC分类号： C09K11/025 ,  C08B37/003 ,  C09K11/06 ,  G01N33/533

摘要： The invention discloses the microspheres comprising the following structure in the composition thereof and the uses as tracers and fluorescent standard calibration products. wherein, groups A, B and C are linked to hydrocarbon chain , and at least one of A, B and C has the structure of —N═R—, in which R is a residue after the reaction of an aldehyde-type cross-linking substance, and the others of A, B and C are —NH2 structure. The microspheres comprising the above structure have an obvious phenomenon of self-fluorescence, and the fluorescence output of each microsphere is significantly larger than the fluorescence outputted by the microsphere embedded with fluorescent substances. The microspheres have toleration to light-bleach and the dependency on other environments without modifying the surface property of the microspheres themselves. The invention further discloses the preparation method of the above fluorescent microspheres. The fluorescence color of the fluorescent microspheres can be adjusted by selecting different aldehyde-type substances or the combinations thereof, and the fluorescence intensity of the microspheres can be controlled not only by the addition of the aldehyde-type substances and the time of the cross-linking reaction, but also by adjusting the size of the particle diameters of the microspheres.

摘要翻译： 本发明公开了在其组成中包含以下结构的微球和用作示踪剂和荧光标准校准产物的用途。 其中A，B和C基团与烃链连接，A，B和C中的至少一个具有-N = R-的结构，其中R是醛类交联反应后的残基， 连接物质，其他的A，B和C是-NH 2结构。 包含上述结构的微球具有明显的自发荧光现象，每个微球的荧光输出明显大于嵌入荧光物质的微球输出的荧光。 微球具有对漂白剂的耐受性和对其它环境的依赖性，而不改变微球本身的表面性质。 本发明还公开了上述荧光微球的制备方法。 可以通过选择不同的醛类物质或其组合来调节荧光微球的荧光颜色，并且不仅可以通过加入醛类物质和交联时间来控制微球的荧光强度， 连接反应，而且还通过调节微球的粒径的大小。

公开(公告)号：US08460638B2

公开(公告)日：2013-06-11

申请号：US12441894

申请日：2007-09-13

申请人： Guanghui Ma ,  Zhiguo Su ,  Wei Wei ,  Lianyan Wang ,  Qiang Wei

发明人： Guanghui Ma ,  Zhiguo Su ,  Wei Wei ,  Lianyan Wang ,  Qiang Wei

IPC分类号： A61B5/00

CPC分类号： C09K11/025 ,  C08B37/003 ,  C09K11/06 ,  G01N33/533

摘要： A method of fluorescence imaging is disclosed that uses a non-labeled fluorescent microsphere comprising a primary amine polymer, wherein the microsphere does not contain an additional fluorescent substance, and wherein at least one primary amine group in the primary amine polymer has the structure of —N═R—, in which R is a residue after the reaction of an aldehyde-type cross-linking substance, and the other primary amine groups have the structure of —NH2. The fluorescence color of the microspheres can be adjusted by selecting different aldehyde-type cross-linking substances or combinations, and the fluorescence intensity of the microspheres can be controlled not only by the addition of the aldehyde-type cross-linking substances and the time of the cross-linking reaction, but also by adjusting the diameters of the microspheres.

摘要翻译： 公开了一种荧光成像方法，其使用包含伯胺聚合物的非标记荧光微球，其中微球不含另外的荧光物质，并且其中伯胺聚合物中的至少一个伯胺基具有以下结构： N = R-，其中R是醛类交联物质反应后的残基，其它伯胺基具有-NH 2的结构。 可以通过选择不同的醛类交联物质或组合来调节微球的荧光颜色，并且不仅可以通过加入醛型交联物质来控制微球的荧光强度和时间 交联反应，也可以通过调节微球的直径。

公开(公告)号：US08506812B2

公开(公告)日：2013-08-13

申请号：US13555668

申请日：2012-07-23

申请人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

发明人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

IPC分类号： B01D33/44 ,  B01D29/075

CPC分类号： B01D65/08 ,  B01D61/145 ,  B01D61/147 ,  B01D63/082 ,  B01D2321/26

摘要： The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components. The method and equipment of the present invention can be widely applied in various membrane techniques, in particular in a membrane separation process for concentrating biomacromolecule and organic micromolecule products such as sugars, organic acids and polypeptides etc.

摘要翻译： 本发明涉及一种膜分离方法和相关设备，特别涉及在膜过滤过程中利用浓度极化的膜分离方法和设备，特别涉及浓缩过程和设备以及特别用于绘制浓缩极化的抽屉 层。 从膜表面直接除去浓缩极化层不仅降低了浓度极化对膜分离的不利影响，而且获得了浓缩的保留成分，从而显着提高了保持膜通量的能力，解决了浓度极化和膜污染的双胎问题 在膜分离过程中，并且实现保留成分的高效浓缩。 本发明的方法和设备可以广泛地应用于各种膜技术中，特别是在用于浓缩生物大分子和有机小分子产物如糖，有机酸和多肽等的膜分离方法中。

公开(公告)号：US20120285889A1

公开(公告)日：2012-11-15

申请号：US13555668

申请日：2012-07-23

申请人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

发明人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

IPC分类号： B01D61/14 ,  B01D61/36

CPC分类号： B01D65/08 ,  B01D61/145 ,  B01D61/147 ,  B01D63/082 ,  B01D2321/26

摘要： The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components. The method and equipment of the present invention can be widely applied in various membrane techniques, in particular in a membrane separation process for concentrating biomacromolecule and organic micromolecule products such as sugars, organic acids and polypeptides etc.

摘要翻译： 本发明涉及一种膜分离方法和相关设备，特别涉及在膜过滤过程中利用浓度极化的膜分离方法和设备，特别涉及浓缩过程和设备以及特别用于绘制浓缩极化的抽屉 层。 从膜表面直接除去浓缩极化层不仅降低了浓度极化对膜分离的不利影响，而且获得了浓缩的保留成分，从而显着提高了保持膜通量的能力，解决了浓度极化和膜污染的双胎问题 在膜分离过程中，并且实现保留成分的高效浓缩。 本发明的方法和设备可以广泛地应用于各种膜技术中，特别是在用于浓缩生物大分子和有机小分子产物如糖，有机酸和多肽等的膜分离方法中。

公开(公告)号：US20100044310A1

公开(公告)日：2010-02-25

申请号：US12443797

申请日：2008-03-05

申请人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

发明人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

IPC分类号： B01D65/08 ,  B01D63/00 ,  B01D65/00

CPC分类号： B01D65/08 ,  B01D61/145 ,  B01D61/147 ,  B01D63/082 ,  B01D2321/26

摘要： The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components. The method and equipment of the present invention can be widely applied in various membrane techniques, in particular in a membrane separation process for concentrating biomacromolecule and organic micromolecule products such as sugars, organic acids and polypeptides etc.

摘要翻译： 本发明涉及一种膜分离方法和相关设备，特别涉及在膜过滤过程中利用浓度极化的膜分离方法和设备，特别涉及浓缩过程和设备以及特别用于绘制浓缩极化的抽屉 层。 从膜表面直接除去浓缩极化层不仅降低了浓度极化对膜分离的不利影响，而且获得了浓缩的保留成分，从而显着提高了保持膜通量的能力，解决了浓度极化和膜污染的双胎问题 在膜分离过程中，并且实现保留成分的高效浓缩。 本发明的方法和设备可以广泛地应用于各种膜技术中，特别是在用于浓缩生物大分子和有机小分子产物如糖，有机酸和多肽等的膜分离方法中。

公开(公告)号：US08252184B2

公开(公告)日：2012-08-28

申请号：US12443797

申请日：2008-03-05

申请人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

发明人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

IPC分类号： B01D29/075 ,  B01D33/44

CPC分类号： B01D65/08 ,  B01D61/145 ,  B01D61/147 ,  B01D63/082 ,  B01D2321/26

摘要： The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components. The method and equipment of the present invention can be widely applied in various membrane techniques, in particular in a membrane separation process for concentrating biomacromolecule and organic micromolecule products such as sugars, organic acids and polypeptides etc.

摘要翻译： 本发明涉及一种膜分离方法和相关设备，特别涉及在膜过滤过程中利用浓度极化的膜分离方法和设备，特别涉及浓缩过程和设备以及特别用于绘制浓缩极化的抽屉 层。 从膜表面直接除去浓缩极化层不仅降低了浓度极化对膜分离的不利影响，而且获得了浓缩的保留成分，从而显着提高了保持膜通量的能力，解决了浓度极化和膜污染的双胎问题 在膜分离过程中，并且实现保留成分的高效浓缩。 本发明的方法和设备可以广泛地应用于各种膜技术中，特别是在用于浓缩生物大分子和有机小分子产物如糖，有机酸和多肽等的膜分离方法中。