公开(公告)号：US20180087003A1

公开(公告)日：2018-03-29

申请号：US15830772

申请日：2017-12-04

Applicant: H R D Corporation

Inventor： Abbas Hassan ,  Aziz Hassan ,  Gregory G. Borsinger ,  Rayford Gaines Anthony

IPC: C11B1/10 ,  C12P7/64 ,  A23D9/00 ,  C11B1/02 ,  C10L1/02 ,  C12M1/00 ,  C11B1/04 ,  B01F7/00 ,  B01F13/10

CPC classification number: C11B1/10 ,  A23D9/00 ,  A23V2002/00 ,  B01F7/00766 ,  B01F13/1016 ,  C10L1/026 ,  C10L2200/0476 ,  C10L2290/54 ,  C11B1/02 ,  C11B1/04 ,  C12M21/02 ,  C12M23/18 ,  C12M47/06 ,  C12P7/6463 ,  Y02E50/13 ,  Y02P20/142 ,  A23V2250/19 ,  A23V2250/202 ,  A23V2300/14 ,  A23V2300/41

Abstract: Herein disclosed is a method of processing a medium containing algae microorganisms to produce algal oil and by-products, comprising providing the medium containing algae microorganisms; passing the medium through a rotor-stator high shear device; disintegrating cell walls of and intracellular organelles in the algae microorganisms to release algal oil and by-products; and removing the algae medium from an outlet of the high shear device. In an embodiment, disintegration is enhanced by a penetrating gas capable of permeating the cell wall. In an embodiment, enhancement is accomplished by super-saturation of the penetrating gas in the medium or increased gas pressure in a vessel. In an embodiment, the penetrating gas is different from the gas produced by the cell during respiration. A suitable system is also discussed in this disclosure.

公开(公告)号：US20170051230A1

公开(公告)日：2017-02-23

申请号：US14831453

申请日：2015-08-20

Applicant: H R D Corporation

Inventor： Abbas Hassan ,  Aziz Hassan ,  Gregory G. Borsinger ,  Rayford Gaines Anthony

IPC: C11B1/10 ,  C10L1/02 ,  C12M1/00 ,  C12M1/42 ,  C11B1/04 ,  C12M1/26

CPC classification number: C11B1/10 ,  A23D9/00 ,  A23V2002/00 ,  B01F7/00766 ,  B01F13/1016 ,  C10L1/026 ,  C10L2200/0476 ,  C10L2290/54 ,  C11B1/02 ,  C11B1/04 ,  C12M21/02 ,  C12M23/18 ,  C12M47/06 ,  C12P7/6463 ,  A23V2250/19 ,  A23V2250/202 ,  A23V2300/14 ,  A23V2300/41

Abstract: Herein disclosed is a method of processing a medium containing algae microorganisms to produce algal oil and by-products, comprising providing the medium containing algae microorganisms; passing the medium through a rotor-stator high shear device; disintegrating cell walls of and intracellular organelles in the algae microorganisms to release algal oil and by-products; and removing the algae medium from an outlet of the high shear device. In an embodiment, disintegration is enhanced by a penetrating gas capable of permeating the cell wall. In an embodiment, enhancement is accomplished by super-saturation of the penetrating gas in the medium or increased gas pressure in a vessel. In an embodiment, the penetrating gas is different from the gas produced by the cell during respiration. A suitable system is also discussed in this disclosure.

Abstract translation: 本文公开了一种处理含有藻类微生物以产生藻油和副产物的培养基的方法，包括提供含有藻类微生物的培养基; 使介质通过转子 - 定子高剪切装置; 在藻类微生物中分解细胞壁和细胞内细胞器以释放藻油和副产物; 以及从高剪切装置的出口去除藻类培养基。 在一个实施方案中，通过能够渗透细胞壁的穿透性气体增强了崩解。 在一个实施例中，通过介质中的穿透气体的过饱和或容器中的气体压力增加来实现增强。 在一个实施方案中，穿透气体与呼吸期间由细胞产生的气体不同。 在本公开中还讨论了合适的系统。

公开(公告)号：US09862910B2

公开(公告)日：2018-01-09

申请号：US14831453

申请日：2015-08-20

Applicant: H R D Corporation

Inventor： Abbas Hassan ,  Aziz Hassan ,  Gregory G. Borsinger ,  Rayford Gaines Anthony

IPC: A23D9/00 ,  B01F7/00 ,  B01F13/10 ,  C11B1/02 ,  C11B1/04 ,  C11B1/10 ,  C10L1/02 ,  C12M1/00 ,  C12P7/64

CPC classification number: C11B1/10 ,  A23D9/00 ,  A23V2002/00 ,  B01F7/00766 ,  B01F13/1016 ,  C10L1/026 ,  C10L2200/0476 ,  C10L2290/54 ,  C11B1/02 ,  C11B1/04 ,  C12M21/02 ,  C12M23/18 ,  C12M47/06 ,  C12P7/6463 ,  A23V2250/19 ,  A23V2250/202 ,  A23V2300/14 ,  A23V2300/41

Abstract: Herein disclosed is a method of processing a medium containing algae microorganisms to produce algal oil and by-products, comprising providing the medium containing algae microorganisms; passing the medium through a rotor-stator high shear device; disintegrating cell walls of and intracellular organelles in the algae microorganisms to release algal oil and by-products; and removing the algae medium from an outlet of the high shear device. In an embodiment, disintegration is enhanced by a penetrating gas capable of permeating the cell wall. In an embodiment, enhancement is accomplished by super-saturation of the penetrating gas in the medium or increased gas pressure in a vessel. In an embodiment, the penetrating gas is different from the gas produced by the cell during respiration. A suitable system is also discussed in this disclosure.

公开(公告)号：US20180087019A1

公开(公告)日：2018-03-29

申请号：US15830780

申请日：2017-12-04

Applicant: H R D Corporation

Inventor： Abbas Hassan ,  Aziz Hassan ,  Gregory G. Borsinger ,  Rayford Gaines Anthony

IPC: C12M1/42 ,  C12M1/00 ,  C12N1/12 ,  C12P7/64 ,  C12N1/06

CPC classification number: C12M35/04 ,  C12M21/02 ,  C12M21/12 ,  C12M29/06 ,  C12N1/066 ,  C12N1/12 ,  C12P7/6463 ,  Y02E50/13

Abstract: Herein disclosed is a method of generating products from microorganisms, comprising super-saturating a liquid medium with a gas consumable by the microorganisms in a high shear device operating at a shear rate of greater than 1,000,000 s−1 to produce a gas-super-saturated (GSS) medium, wherein the GSS medium maintains a GSS level for at least 10 minutes; feeding the GSS medium to microorganisms; allowing the microorganisms to grow by consuming the gas and generate products via photosynthesis or chemosynthesis; and recovering the products. In an embodiment, the microorganisms are genetically modified. In an embodiment, the microorganisms include bacteria, protozoa, algae, or fungi, or a combination thereof. In an embodiment, the gas consumable by the microorganisms is selected from the group consisting of carbon dioxide, nitrogen, air, oxygen, methane, and combinations thereof. A suitable system is also discussed in this disclosure.

公开(公告)号：US09873859B2

公开(公告)日：2018-01-23

申请号：US14831476

申请日：2015-08-20

Applicant: H R D Corporation

Inventor： Abbas Hassan ,  Aziz Hassan ,  Gregory G. Borsinger ,  Rayford Gaines Anthony

IPC: C12M1/00 ,  C12M1/42 ,  C12N1/06 ,  C12N1/12 ,  C12P7/64

CPC classification number: C12M35/04 ,  C12M21/02 ,  C12M21/12 ,  C12M29/06 ,  C12N1/066 ,  C12N1/12 ,  C12P7/6463

Abstract: Herein disclosed is a method of generating products from microorganisms, comprising super-saturating a liquid medium with a gas consumable by the microorganisms in a high shear device operating at a shear rate of greater than 1,000,000 s−1 to produce a gas-super-saturated (GSS) medium, wherein the GSS medium maintains a GSS level for at least 10 minutes; feeding the GSS medium to microorganisms; allowing the microorganisms to grow by consuming the gas and generate products via photosynthesis or chemosynthesis; and recovering the products. In an embodiment, the microorganisms are genetically modified. In an embodiment, the microorganisms include bacteria, protozoa, algae, or fungi, or a combination thereof. In an embodiment, the gas consumable by the microorganisms is selected from the group consisting of carbon dioxide, nitrogen, air, oxygen, methane, and combinations thereof. A suitable system is also discussed in this disclosure.

公开(公告)号：US20170051244A1

公开(公告)日：2017-02-23

申请号：US14831476

申请日：2015-08-20

Applicant: H R D Corporation

Inventor： Abbas Hassan ,  Aziz Hassan ,  Gregory G. Borsinger ,  Rayford Gaines Anthony

IPC: C12M1/42 ,  C12M1/26 ,  C12M1/00 ,  C12P7/64

CPC classification number: C12M35/04 ,  C12M21/02 ,  C12M21/12 ,  C12M29/06 ,  C12N1/066 ,  C12N1/12 ,  C12P7/6463

Abstract: Herein disclosed is a method of generating products from microorganisms, comprising super-saturating a liquid medium with a gas consumable by the microorganisms in a high shear device operating at a shear rate of greater than 1,000,000 s−1 to produce a gas-super-saturated (GSS) medium, wherein the GSS medium maintains a GSS level for at least 10 minutes; feeding the GSS medium to microorganisms; allowing the microorganisms to grow by consuming the gas and generate products via photosynthesis or chemosynthesis; and recovering the products. In an embodiment, the microorganisms are genetically modified. In an embodiment, the microorganisms include bacteria, protozoa, algae, or fungi, or a combination thereof. In an embodiment, the gas consumable by the microorganisms is selected from the group consisting of carbon dioxide, nitrogen, air, oxygen, methane, and combinations thereof. A suitable system is also discussed in this disclosure.

Abstract translation: 本文公开了一种从微生物产生产物的方法，其包括在以大于1,000,000s -1的剪切速率操作的高剪切装置中用微生物消耗的气体超饱和液体培养基以产生气体超饱和 （GSS）培养基，其中GSS培养基维持GSS水平至少10分钟; 将GSS培养基喂给微生物; 通过消耗气体并通过光合作用或化学合成产生产物，使微生物生长; 并恢复产品。 在一个实施方案中，微生物被遗传修饰。 在一个实施方案中，微生物包括细菌，原生动物，藻类或真菌，或其组合。 在一个实施方案中，由微生物消耗的气体选自二氧化碳，氮气，空气，氧气，甲烷及其组合。 在本公开中也讨论了合适的系统。