公开(公告)号：US20190022647A1

公开(公告)日：2019-01-24

申请号：US16070602

申请日：2016-02-22

Applicant: HITACHI, LTD.

Inventor： Mayu AOKI ,  Kenichi TAKEDA ,  Kunio HARADA

IPC: B01L3/00 ,  G01N27/414 ,  G01N33/543

Abstract: A biological sample analysis chip including a first substrate, a membrane disposed on the first substrate, a first liquid tank which is provided with a first electrode, a plurality of second liquid tanks each of which is provided with at least one flow path and a second electrode; and a second substrate disposed below the first substrate, in which the plurality of second liquid tanks are substantially insulated from each other, the membrane disposed on the first substrate is disposed between the first liquid tank and the plurality of second liquid tanks so as to form a portion of the first liquid tank and a portion of the plurality of second liquid tanks, and the second substrate is provided with the at least one flow path and the second electrode so as to form a portion of the plurality of second liquid tanks.

公开(公告)号：US20180298435A1

公开(公告)日：2018-10-18

申请号：US15755957

申请日：2016-07-05

Applicant: Hitachi, Ltd.

Inventor： Junko TANAKA ,  Yoshinobu KOHARA ,  Kunio HARADA ,  Yu ISHIGE

IPC: C12Q1/6869 ,  C12M1/00 ,  G06F19/10 ,  B01J19/00

Abstract: The present invention was made to provide novel methods for detecting DNA and devices therefor. Specifically, the present invention is a method of detecting DNA including the steps of: adding, to an oil, a fluorescently-labeled probe, a DNA intercalator, and a DNA solution containing a target DNA to produce droplets; performing PCR on the droplets; and measuring fluorescence from the fluorescently-labeled probe and fluorescence from the DNA intercalator, wherein the DNA solution has a concentration at which each droplet is produced so as to contain one or less target DNA molecule. In addition, the present invention is a DNA detection device including a droplet production unit, a thermal cycler unit, and a fluorescence detection unit which perform these steps.

公开(公告)号：US20220155227A1

公开(公告)日：2022-05-19

申请号：US17435310

申请日：2020-02-26

Applicant: HITACHI, LTD.

Inventor： Tatsuo NAKAGAWA ,  Junko TANAKA ,  Yuzuru SHIMAZAKI ,  Kunio HARADA

IPC: G01N21/64 ,  G01N21/03 ,  G01N21/15

Abstract: A digital PCR measuring apparatus capable of measuring a melting curve with high accuracy is provided. The digital PCR measuring apparatus includes a temperature adjuster that controls a temperature of a sample container including a plurality of minute regions, a fluorescence measurement part that measures fluorescence intensity of a plurality of minute regions, and a controller that controls the temperature adjuster and the fluorescence measurement part. The controller controls the temperature adjuster to raise a temperature of the sample container, and, after removing air bubbles generated in the sample container, measures fluorescence intensity of a plurality of minute regions while controlling the temperature adjuster to lower the temperature of the sample container, and measures a melting curve of a plurality of the minute regions.

公开(公告)号：US20210130877A1

公开(公告)日：2021-05-06

申请号：US16973990

申请日：2019-05-20

Applicant: HITACHI, LTD.

Inventor： Junko TANAKA ,  Tatsuo NAKAGAWA ,  Yuzuru SHIMAZAKI ,  Kunio HARADA

IPC: C12Q1/6825 ,  B01L3/00

Abstract: The present invention addresses the problem of providing a novel digital PCR analysis method. One embodiment of the novel digital PCR analysis method is a DNA detection method including the steps of: partitioning a DNA solution containing a fluorescent-labeled probe or a DNA intercalator and a target DNA to be detected into a plurality of compartments; carrying out a nucleic acid amplification reaction in the compartments; measuring a fluorescence intensity in association with a temperature change; calculating a melting temperature of a DNA double strand based on a change in the fluorescence intensity in association with the temperature change; and calculating a ratio of a fluorescence intensity at a second temperature that is lower than a first temperature in association with the temperature change to that at the first temperature.

公开(公告)号：US20150068627A1

公开(公告)日：2015-03-12

申请号：US14478453

申请日：2014-09-05

Applicant: Hitachi, Ltd.

Inventor： Kunio HARADA ,  Akihiro FURUKAWA ,  Masakazu SUGAYA ,  Norihito KUNO ,  Hisashi MURATA

IPC: F16K11/085 ,  F16H25/12

CPC classification number: F16K11/0853 ,  F16H25/12 ,  Y10T74/18312 ,  Y10T137/86871

Abstract: An actuator device includes a pin that performs translatory motion along a groove of a cam to which a three-way stopcock is connected, the cam that performs rotary motion, and a control unit that causes the pin to perform the translatory motion along an extending direction of the cam, and rotates the cam with respect to a central axis of rotation, thereby to divert a passage of the three-way stopcock. A first, second, third, and fourth grooves that form the groove have a linked shape. Directions of inclinations of the first inclination angle and the third inclination angle are opposite with respect to the central axis, and directions of inclinations of the second inclination angle and the fourth inclination angle are opposite with respect to the central axis.

Abstract translation: 致动器装置包括一个销，它沿着连接有三通旋塞的凸轮的凹槽执行平移运动，执行旋转运动的凸轮以及使销沿着延伸方向执行平移运动的控制单元 并且相对于中心旋转轴线旋转凸轮，从而转动三通旋塞的通道。 形成凹槽的第一，第二，第三和第四凹槽具有连接的形状。 第一倾斜角度和第三倾斜角度的倾斜方向相对于中心轴线相反，并且第二倾斜角度和第四倾斜角度的倾斜方向相对于中心轴线相反。

公开(公告)号：US20190293625A1

公开(公告)日：2019-09-26

申请号：US16464835

申请日：2017-10-12

Applicant: HITACHI, LTD.

Inventor： Mayu AOKI ,  Itaru YANAGI ,  Kunio HARADA ,  Kenichi TAKEDA

IPC: G01N33/487 ,  G01N27/447

Abstract: A method includes a step of introducing a solution between a substrate with a membrane in which the membrane is provided so as to close an opening and a substrate provided with an independent electrode in which the independent electrode is provided, a step of pressure bonding the substrate with the membrane and the substrate with the independent electrode through a partition wall, and a step of forming a sealed liquid tank surrounded by at least the membrane and the partition wall by the pressure bonding, and arraying of a solid-state type nanopore sequencer is simply performed.

公开(公告)号：US20150324626A1

公开(公告)日：2015-11-12

申请号：US14802425

申请日：2015-07-17

Applicant: HITACHI, LTD.

Inventor： Miyuki KONO ,  Shin-ichiro UMEMURA ,  Takafumi MIYATAKE ,  Kunio HARADA ,  Yoshitoshi ITO ,  Hironori UEKI

IPC: G06K9/00 ,  H04N5/235

CPC classification number: H04N5/2354 ,  G06K9/00 ,  G06K9/00013 ,  G06K9/00375 ,  G06K2009/0006 ,  G06K2009/00932

Abstract: A personal identification system, which uses a vein pattern of a finger, optimizes the amount of light of a light source based on a captured finger image and emphasizes the vein pattern during image processing for identification.

Abstract translation: 使用手指的静脉图案的个人识别系统基于捕获的手指图像优化光源的光量，并且在用于识别的图像处理期间强调静脉图案。