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    THERMOSTABLE GLYCOSIDE HYDROLASE
    1.
    发明申请
    THERMOSTABLE GLYCOSIDE HYDROLASE 有权

    公开(公告)号：US20160340663A1

    公开(公告)日：2016-11-24

    申请号：US15156759

    申请日：2016-05-17

    申请人： HONDA MOTOR CO., LTD.

    发明人： Migiwa SUDA ,  Jiro OKUMA ,  Asuka YAMAGUCHI ,  Yoshitsugu HIROSE ,  Yasuhiro KONDO ,  Masaru SATO ,  Daisuke SHIBATA

    IPC分类号： C12N9/42 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14

    CPC分类号： C12N9/2437 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01004

    摘要： A thermostable glycoside hydrolase having a glycoside hydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; (D) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 51 to 485 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA at least under conditions of 65° C. and pH 4.0, and a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; or (E) a polypeptide in which are linked, either directly or via a linker, a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from position 51 to position 485 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of PSA at least under conditions of 65° C. and pH 4.0, and a polypeptide including an amino acid sequence having 80% or greater sequence identity with the region composed of amino acids from positions 530 to 823 in the amino acid sequence represented by SEQ ID NO: 1 and having hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0.

    THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3
    2.
    发明申请
    THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3 有权
    GHG家族可热稳定的β-XYLOSIDASE 3

    公开(公告)号：US20160032265A1

    公开(公告)日：2016-02-04

    申请号：US14813894

    申请日：2015-07-30

    申请人： HONDA MOTOR CO., LTD.

    发明人： Yasuhiro KONDO ,  Yoshitsugu HIROSE ,  Asuka YAMAGUCHI ,  Migiwa SUDA ,  Jiro OKUMA ,  Tomohiko KATO ,  Daisuke SHIBATA

    IPC分类号： C12N9/24 ,  C12P19/02 ,  C12P19/14

    CPC分类号： C12N9/248 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/2445 ,  C12Y302/01037

    摘要： A thermostable β-xylosidase including a β-xylosidase catalytic domain, the β-xylosidase catalytic domain including: (A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid is deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-xylopyranoside as a substrate at least under conditions of a temperature of 85° C. and a pH of 6.0; or (C) a polypeptide including an amino acid sequence having at least 80% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-xylopyranoside as a substrate at least under conditions of a temperature of 85° C. and a pH of 6.0.

    摘要翻译： 包含α-脱氧核糖酶催化结构域的热稳定性 - 木糖苷酶，所述β-氧化酶催化结构域包括：（A）包含由SEQ ID NO：1表示的氨基酸序列的多肽; （B）包含氨基酸序列的多肽，其中至少一个氨基酸缺失，取代或添加在SEQ ID NO：1所示的氨基酸序列中，并且具有使用对硝基苯基-β 至少在温度为85℃，pH为6.0的条件下作为底物， 或（C）包含与SEQ ID NO：1所示的氨基酸序列具有至少80％序列同一性的氨基酸序列的多肽，并且使用对硝基苯基-β-吡喃木糖作为底物具有水解活性 至少在温度为85℃，pH为6.0的条件下。

    HYPERTHERMOSTABLE ENDOGLUCANASE BELONGING TO GH FAMILY 12
    4.
    发明申请
    HYPERTHERMOSTABLE ENDOGLUCANASE BELONGING TO GH FAMILY 12 有权
    高可重复性内切酶与GH家族相关12

    公开(公告)号：US20160122791A1

    公开(公告)日：2016-05-05

    申请号：US14816603

    申请日：2015-08-03

    申请人： HONDA MOTOR CO., LTD.

    发明人： Asuka YAMAGUCHI ,  Jiro OKUMA ,  Yoshitsugu HIROSE ,  Migiwa SUDA ,  Yasuhiro KONDO ,  Tomohiko KATO ,  Daisuke SHIBATA

    IPC分类号： C12P19/14 ,  C12P19/02 ,  C12N9/42

    CPC分类号： C12P19/14 ,  C12N9/2437 ,  C12P19/02 ,  C12Y302/01004

    摘要： A hyperthermostable endoglucanase including an endoglucanase catalytic domain, the endoglucanase catalytic domain including: (A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid is deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-cellobioside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5; or (C) a polypeptide including an amino acid sequence having at least 70% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-cellobioside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5.

    摘要翻译： 包含内切葡聚糖酶催化结构域的超热稳定内切葡聚糖酶，所述内切葡聚糖酶催化结构域包括：（A）包含由SEQ ID NO：1表示的氨基酸序列的多肽; （B）包含氨基酸序列的多肽，其中至少一个氨基酸缺失，取代或添加在SEQ ID NO：1所示的氨基酸序列中，并且具有使用对硝基苯基-β 至少在温度为100℃，pH为5.5的条件下，作为底物的纤维二糖苷; 或（C）多肽，其包含与SEQ ID NO：1所示的氨基酸序列具有至少70％序列同一性的氨基酸序列，并且使用对硝基苯基-β-D-纤维二糖苷作为底物具有水解活性 至少在温度为100℃，pH为5.5的条件下。

    THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3
    5.
    发明申请
    THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3 有权
    GHG家族可热稳定的β-XYLOSIDASE 3

    公开(公告)号：US20160076017A1

    公开(公告)日：2016-03-17

    申请号：US14854562

    申请日：2015-09-15

    申请人： Honda Motor Co., Ltd.

    发明人： Yasuhiro KONDO ,  Jiro OKUMA ,  Yoshitsugu HIROSE ,  Asuka YAMAGUCHI ,  Migiwa SUDA ,  Tomohiko KATO ,  Daisuke SHIBATA

    IPC分类号： C12N9/24 ,  C12P19/14 ,  C12P19/02 ,  C12N9/42

    CPC分类号： C12N9/2477 ,  C07K14/435 ,  C12N9/2445 ,  C12N9/2485 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01037

    摘要： A thermostable β-xylosidase, having a β-xylosidase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0, or (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0.

    摘要翻译： 一种具有β-脱氧核苷酸酶催化结构域的热稳定性 - 木糖苷酶，包括：（A）包含SEQ ID NO：1,3或5所示氨基酸序列的多肽，（B）包含氨基酸序列的多肽 其中至少一个氨基酸已被缺失，取代或添加在SEQ ID NO：1,3或5所示的氨基酸序列中，并且具有对对硝基苯基-β-吡喃木糖苷底物的水解活性 至少在80℃和pH4.0的条件下，或（C）包含与SEQ ID NO：1,3或5所示氨基酸序列具有80％或更高的氨基酸序列的氨基酸序列的多肽，并具有水解 至少在80℃和pH4.0的条件下，对对硝基苯基-β-D-吡喃木糖苷的底物的活性。

    HYPERTHERMOSTABLE ENDOGLUCANASE
    7.
    发明申请
    HYPERTHERMOSTABLE ENDOGLUCANASE 有权
    高可重复性内切酶

    公开(公告)号：US20170051264A1

    公开(公告)日：2017-02-23

    申请号：US15238370

    申请日：2016-08-16

    申请人： HONDA MOTOR CO., LTD.

    发明人： Migiwa SUDA ,  Jiro OKUMA ,  Asuka YAMAGUCHI ,  Yoshitsugu HIROSE ,  Yasuhiro KONDO ,  Daisuke SHIBATA ,  Masaru SATO

    IPC分类号： C12N9/42 ,  C12P19/02 ,  C12P19/14

    CPC分类号： C12N9/2437 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01004

    摘要： A hyperthermostable endoglucanase, having an endoglucanase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1 or 2, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of 90° C. and pH 5.5, or (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of 90° C. and pH 5.5.

    摘要翻译： 一种具有内切葡聚糖酶催化结构域的超热稳定内切葡聚糖酶，包括：（A）包含SEQ ID NO：1或2所示氨基酸序列的多肽，（B）包含至少一个氨基酸已经被 至少在90℃和pH5.5的条件下具有对羧甲基纤维素底物的水解活性，或者（C）多肽的氨基酸序列， 包括与SEQ ID NO：1或2表示的氨基酸序列具有80％以上序列同一性的氨基酸序列，并且至少在90℃和pH5.5的条件下具有对羧甲基纤维素底物的水解活性。

    THERMOSTABLE CELLOBIOHYDROLASE
    8.
    发明申请
    THERMOSTABLE CELLOBIOHYDROLASE 审中-公开
    可热释放的碳水化合物

    公开(公告)号：US20160264952A1

    公开(公告)日：2016-09-15

    申请号：US15063941

    申请日：2016-03-08

    申请人： HONDA MOTOR CO., LTD.

    发明人： Jiro OKUMA ,  Migiwa SUDA ,  Asuka YAMAGUCHI ,  Yoshitsugu HIROSE ,  Yasuhiro KONDO ,  Masaru SATO ,  Daisuke SHIBATA

    IPC分类号： C12N9/42 ,  C12P19/02 ,  C12P19/14

    CPC分类号： C12N9/2437 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01091

    摘要： A thermostable cellobiohydrolase, having a cellobiohydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1 or 2, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 75° C. and pH 5.5, or (C) a polypeptide including an amino acid sequence having 75% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 75° C. and pH 5.5.

    摘要翻译： 一种具有纤维二糖水解酶催化结构域的热稳定纤维二糖水解酶，包括：（A）包含SEQ ID NO：1或2所示氨基酸序列的多肽，（B）包含至少一个氨基酸的氨基酸序列的多肽 或者在至少在75℃和pH5.5的条件下具有对磷酸溶胀的Avicel底物的水解活性，或者（C） 至少在75℃的条件下，具有与SEQ ID NO：1或2表示的氨基酸序列具有75％以上序列同一性的氨基酸序列，并且具有抗磷酸溶胀Avicel底物的水解活性的多肽。 和pH 5.5。

    THERMOSTABLE BETA-GLUCOSIDASE
    9.
    发明申请
    THERMOSTABLE BETA-GLUCOSIDASE 有权
    热稳定的β-葡萄糖苷酶

    公开(公告)号：US20160076015A1

    公开(公告)日：2016-03-17

    申请号：US14850407

    申请日：2015-09-10

    申请人： Honda Motor Co., Ltd.

    发明人： Yoshitsugu HIROSE ,  Takahiro GUNJI ,  Asuka YAMAGUCHI ,  Migiwa SUDA ,  Jiro OKUMA ,  Yasuhiro KONDO ,  Tomohiko KATO ,  Daisuke SHIBATA

    IPC分类号： C12N9/42 ,  C12P19/02 ,  C12P19/14

    CPC分类号： C12N9/2445 ,  C12N9/2434 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021

    摘要： A thermostable β-glucosidase including a β-glucosidase catalytic domain, the β-glucosidase catalytic domain including: (A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid is deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-glucopyranoside as a substrate at least under conditions of a temperature of 85° C. and a pH of 7.5; or (C) a polypeptide including an amino acid sequence having at least 60% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-glucopyranoside as a substrate at least under conditions of a temperature of 85° C. and a pH of 7.5.

    摘要翻译： 包含葡糖苷酶催化结构域的热稳定性 - 葡萄糖苷酶，所述β-葡糖苷酶催化结构域包括：（A）包含由SEQ ID NO：1表示的氨基酸序列的多肽; （B）包含氨基酸序列的多肽，其中至少一个氨基酸缺失，取代或添加在SEQ ID NO：1所示的氨基酸序列中，并且具有使用对硝基苯基-β 至少在温度为85℃，pH为7.5的条件下作为底物; 或（C）包含与SEQ ID NO：1所示的氨基酸序列具有至少60％序列同一性的氨基酸序列的多肽，并且使用对硝基苯基-β-D-吡喃葡萄糖苷作为底物具有水解活性 最少在85℃的温度和7.5的pH条件下。