公开(公告)号：US20120309089A1

公开(公告)日：2012-12-06

申请号：US13118990

申请日：2011-05-31

申请人： HOW TSENG ,  JENG-KUEN TSAI ,  KENG-LIANG OU ,  PO-NIEN CHEN

发明人： HOW TSENG ,  JENG-KUEN TSAI ,  KENG-LIANG OU ,  PO-NIEN CHEN

IPC分类号： C12N5/0775 ,  C12M3/00 ,  C12N5/0735 ,  C12N5/071 ,  C12N5/077

CPC分类号： C12M25/00 ,  C12M25/06 ,  C12M33/00 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/40

摘要： The invention creates a support for cell culture and cell sheet detachment which has a substrate, whose surface is coated with a conjugate having a disulfide bond-containing amino acid as a spacer and a biopolymer enhancing cell attachment, migration or differentation. Unexpectedly, after being seeded on the support, the cells grow to form one or more layers of cell sheets and the cell sheets can be easily detached from the support by adding a reductant to cleave the disulfide bond. Accordingly, the invention provides a simple and non-toxic method for detachment of cell sheets.

摘要翻译： 本发明产生了细胞培养和细胞片剥离的支持物，其具有底物，其表面用具有二硫键的氨基酸作为间隔基的缀合物和生物聚合物增强细胞附着，迁移或分化而被涂覆。 出人意料的是，在接种在载体上之后，细胞生长形成一层或多层细胞片，通过加入还原剂以切割二硫键，可以容易地将细胞片从载体上分离出来。 因此，本发明提供了用于分离细胞片的简单且无毒的方法。