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1.发明授权Plant cell culture for production of natural products with reduced glucosinolate contamination 失效用于生产具有降低的硫代葡萄糖苷污染的天然产物的植物细胞培养物公开(公告)号：US08642340B2
公开(公告)日：2014-02-04
申请号：US13140381
申请日：2009-12-17
IPC分类号： C12N5/00
摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.
摘要翻译： 本发明公开了一种从甘蓝科植物获得具有降低的硫代葡萄糖苷污染的天然产物制剂的方法。 所述方法可以包括从能够产生所需天然产物的植物培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织，以及在液体培养基中培养所选择的愈伤组织。 该方法还可以包括从培养物中回收天然产物。 还公开了获得具有降低的硫代葡萄糖苷污染的甘蓝花青素的方法。 该方法可以包括在液体培养基中培养具有降低的硫代葡萄糖苷产量的红甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。 该方法还可以包括从培养物中降低芥子油苷污染来回收花青素。 最后，描述了几种特异性低硫代葡萄糖苷酸细胞系。
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2.发明申请PLANT CELL CULTURE FOR PRODUCTION OF NATURAL PRODUCTS WITH REDUCED GLUCOSINOLATE CONTAMINATION 失效用于生产具有降低的葡萄糖苷酸盐污染的天然产品的植物细胞培养物公开(公告)号：US20110251408A1
公开(公告)日：2011-10-13
申请号：US13140381
申请日：2009-12-17
IPC分类号： C07D311/60 , C12P17/06
摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.
摘要翻译： 本发明公开了一种从甘蓝科植物获得具有降低的硫代葡萄糖苷污染的天然产物制剂的方法。 所述方法可以包括从能够产生所需天然产物的植物培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织，以及在液体培养基中培养所选择的愈伤组织。 该方法还可以包括从培养物中回收天然产物。 还公开了获得具有降低的硫代葡萄糖苷污染的甘蓝花青素的方法。 该方法可以包括在液体培养基中培养具有降低的硫代葡萄糖苷产量的红甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。 该方法还可以包括从培养物中降低芥子油苷污染来回收花青素。 最后，描述了几种特异性低硫代葡萄糖苷酸细胞系。
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3.发明授权Methods for creating color variation in anthocyanins produced by cell culture 有权通过细胞培养产生的花青素产生颜色变异的方法公开(公告)号：US08962327B2
公开(公告)日：2015-02-24
申请号：US13260667
申请日：2009-05-04
申请人： Sung-Yong Yoon , Helena V. Mathews
发明人： Sung-Yong Yoon , Helena V. Mathews
摘要： Disclosed are methods for obtaining blue anthocyanin pigments and compositions including such pigments. This blue anthocyanin is present at an acidic pH (where anthocyanins are most stable) and may be used as a natural color additive. The methods can include cultivating a plant callus from a plant capable of producing anthocyanin in which the callus is treated with a blue anthocyanin-generating agent at a concentration sufficient to generate callus with blue anthocyanin pigments. The method can also include recovering the blue anthocyanin pigments from the culture. In an example, the blue anthocyanin-generating agent is ammonium molybdate.
摘要翻译： 公开了获得蓝花青素颜料的方法和包括这种颜料的组合物。 这种蓝色花青素存在于酸性pH（其中花青素最稳定），并且可以用作天然色素。 所述方法可以包括从能够产生花青素的植物培养植物愈伤组织，其中用足够产生具有蓝色花青素色素的愈伤组织的浓度的蓝色花青素生成剂处理愈伤组织。 该方法还可以包括从培养物中回收蓝色花青素颜料。 在一个实例中，蓝色花青素生成剂是钼酸铵。
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4.发明申请公开(公告)号：US20120034658A1
公开(公告)日：2012-02-09
申请号：US13260667
申请日：2009-05-04
申请人： Sung-Yong Yoon , Helena Mathews
发明人： Sung-Yong Yoon , Helena Mathews
IPC分类号： C12P19/60
摘要： Disclosed are methods for obtaining blue anthocyanin pigments and compositions including such pigments. This blue anthocyanin is present at an acidic pH (where anthocyanins are most stable) and may be used as a natural color additive. The methods can include cultivating a plant callus from a plant capable of producing anthocyanin in which the callus is treated with a blue anthocyanin-generating agent at a concentration sufficient to generate callus with blue anthocyanin pigments. The method can also include recovering the blue anthocyanin pigments from the culture. In an example, the blue anthocyanin-generating agent is ammonium molybdate.
摘要翻译： 公开了获得蓝花青素颜料的方法和包括这种颜料的组合物。 这种蓝色花青素存在于酸性pH（其中花青素最稳定），并且可以用作天然色素。 所述方法可以包括从能够产生花青素的植物培养植物愈伤组织，其中用足够产生具有蓝色花青素色素的愈伤组织的浓度的蓝色花青素生成剂处理愈伤组织。 该方法还可以包括从培养物中回收蓝色花青素颜料。 在一个实例中，蓝色花青素生成剂是钼酸铵。
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