摘要:
The present invention relates to an enzyme exhibiting endo-beta-1,4-glucanase activity (EC 3.2.1.4), which is a) a polypeptide encoded by the DNA sequence of positions 1 to 2322 of SEQ ID NO: 1; b) a polypeptide produced by culturing a cell comprising the sequence of SEQ ID NO: 1 under conditions wherein the DNA sequence is expressed; c) an endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO: 2; and fragments thereof exhibiting endo-beta-1,4-glucanase activity, and d) a polypeptide having endo-beta-1,4-glucanase activity that is encoded by a polynucleotide that hybridizes with the nucleotide sequence shown in positions 1-2322 of SEQ ID NO: 1, is useful for detergent and textile applications.
摘要翻译:本发明涉及显示内β-1,4-葡聚糖酶活性的酶(EC 3.2.1.4),其是a)由SEQ ID NO:1的1至2322位的DNA序列编码的多肽; b)通过在表达DNA序列的条件下培养包含SEQ ID NO:1的序列的细胞产生的多肽; c)具有与SEQ ID NO:2的位置1至位置773的氨基酸序列具有至少97%同一性的序列的内切-β-1,4-葡聚糖酶; 和表现出内切-β-1,4-葡聚糖酶活性的片段,以及d)具有内切-β-1,4-葡聚糖酶活性的多肽,其由与SEQ ID NO:1-2322所示核苷酸序列杂交的多核苷酸编码 SEQ ID NO:1可用于洗涤剂和纺织品应用。
摘要:
The present invention relates to an enzyme exhibiting endo-beta-1,4-glucanase activity (EC 3.2.1.4), which is a) a polypeptide encoded by the DNA sequence of positions 1 to 2322 of SEQ ID NO: 1; b) a polypeptide produced by culturing a cell comprising the sequence of SEQ ID NO: 1 under conditions wherein the DNA sequence is expressed; c) an endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO: 2; and fragments thereof exhibiting endo-beta-1,4-glucanase activity, and d) a polypeptide having endo-beta-1,4-glucanase activity that is encoded by a polynucleotide that hybridizes with the nucleotide sequence shown in positions 1–2322 of SEQ ID NO: 1, is useful for detergent and textile applications.
摘要翻译:本发明涉及显示内β-1,4-葡聚糖酶活性的酶(EC 3.2.1.4),其是a)由SEQ ID NO:1的1至2322位的DNA序列编码的多肽; b)通过在表达DNA序列的条件下培养包含SEQ ID NO:1的序列的细胞产生的多肽; c)具有与SEQ ID NO:2的位置1至位置773的氨基酸序列具有至少97%同一性的序列的内切-β-1,4-葡聚糖酶; 和表现出内切-β-1,4-葡聚糖酶活性的片段,以及d)具有内切-β-1,4-葡聚糖酶活性的多肽,其由与SEQ ID NO:1-2322所示核苷酸序列杂交的多核苷酸编码 SEQ ID NO:1可用于洗涤剂和纺织品应用。
摘要:
An enzyme exhibiting endo-beta-1,4-glucanase activity (EC 3.2.1.4), which is selected from one of a) a polypeptide encoded by the DNA sequence of positions 1 to 2322 of SEQ ID NO:1; b) a polypeptide produced by culturing a cell comprising the sequence of SEQ ID NO:1 under conditions wherein the DNA sequence is expressed; c) an endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO:2; and fragments thereof exhibiting endo-beta-1,4-glucanase activity, and d) a polypeptide having endo-beta-1,4-glucanase activity that is encoded by a polynucleo-tide that hybridizes with the nucleotide sequence shown in positions 1-2322 of SEQ ID NO:1, is useful for detergent and textile applications.
摘要翻译:显示内β-1,4-葡聚糖酶活性的酶(EC 3.2.1.4),其选自a)由SEQ ID NO:1的1至2322位的DNA序列编码的多肽; b)通过在表达DNA序列的条件下培养包含SEQ ID NO:1的序列的细胞产生的多肽; c)具有与SEQ ID NO:2的位置1至位置773的氨基酸序列具有至少97%同一性的序列的内切-β-1,4-葡聚糖酶; 和表现出内切-β-1,4-葡聚糖酶活性的片段,以及d)具有内切-β-1,4-葡聚糖酶活性的多肽,其与通过多核苷酸编码的多核苷酸杂交,所述多核苷酸与位置1- SEQ ID NO:1的2322可用于洗涤剂和纺织品应用。
摘要:
This invention relates to Alkalophilic Bacillus sp. AC13 microorganisms and cultures, and mutants and variants thereof, and to enzymes obtainable from the microorganism.
摘要:
The present invention is directed to isolated nucleic acid constructs comprising a nucleic acid sequence encoding xylanolytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining xylanolytic enzymes.
摘要:
The present invention relates to proteases obtained from a strain of Bacillus sp. AC13, and having (a) an apparent molecular weight of approximately 30 kD as determined by SDS-PAGE; (b) an isoelectric point of approximately 9.3 as determined by isoelectric focusing on LKB Ampholine PAG plates; (c) a pH optimum above 10 determined at 25.degree. C. with casein as substrate; (d) a temperature optimum in the range of 45.degree.-55.degree. C. determined at pH 9.5 with casein as substrate; and (e) immunochemical properties identical or partially identical to those of a protease derived from Bacillus sp. AC13, NCIMB No. 40482.
摘要:
An isolated nucleic acid constructs encoding cellulytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining cellulytic enzymes.
摘要:
The present invention relates to novel microorganisms, novel enzymes obtainable herefrom, and to a method of producing the novel enzymes. More specifically, the invention relates to novel enzymes obtainable from strains of the novel alkalophilic species Bacillus sp. AC13. Moreover, the invention relates to a method for producing the enzymes of the invention, and to the use of the enzymes in detergents or in the paper pulp industry.
摘要:
A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.
摘要翻译:在pH7下具有至少50%的相对木葡聚糖酶活性和无或不显着的纤维素分解活性的木葡聚糖酶可以例如获得。 来自芽孢杆菌菌株。 包含SEQ ID NO:2的位置30-261所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如地衣芽孢杆菌ATCC 14580,并且可以由包含如SEQ ID NO所示的核苷酸序列的多核苷酸分子编码 :1个从核苷酸88到核苷酸783; 并且包含SEQ ID NO:4的位置1-537所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如琼脂糖琼脂糖凝胶,NCIMB 40482,并且可以由包含SEQ ID NO:4所示的核苷酸序列的多核苷酸分子编码 SEQ ID NO:3从核苷酸1到核苷酸1611.木葡聚糖酶是有用的,例如 在清洁组合物和纤维素纤维的处理中。
摘要:
The present invention relates to novel microorganisms, novel enzymes obtainable herefrom, and to a method of producing the novel enzymes. More specifically, the invention relates to novel enzymes obtainable from strains of the novel alkalophilic species Bacillus sp. AC13. Moreover, the invention relates to a method for producing the enzymes of the invention, and to the use of the enzymes in detergents or in the paper pulp industry.