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公开(公告)号：US09029102B2
公开(公告)日：2015-05-12
申请号：US13175155
申请日：2011-07-01
申请人： Hendrik Van Urk , Philip Harvey Morton , Andrew John Cartwright , Jason Cameron , David James Ballance , Michael Gaston Joseph Grandgeorge , Stephen Berezenko , John Rodney Woodrow , Jean-Luc Bernard Veron
发明人： Hendrik Van Urk , Philip Harvey Morton , Andrew John Cartwright , Jason Cameron , David James Ballance , Michael Gaston Joseph Grandgeorge , Stephen Berezenko , John Rodney Woodrow , Jean-Luc Bernard Veron
IPC分类号： C12N15/14 , C07K14/00 , B01D15/38 , B01D15/36 , C07K14/765
CPC分类号： B01D15/3804 , B01D15/362 , B01D15/363 , C07K14/76 , C07K14/765
摘要： A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilized aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressing a is recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin.
摘要翻译： 提供了一种制备高纯度白蛋白溶液的方法，该方法包括使白蛋白（优选由转化酵母表达和分泌）经历一系列色谱步骤。 优选地，该方法包括以下步骤：阳离子阳离子交换层析，阳性阴离子交换层析，阳性模式亲和层析，负模式亲和色谱法（优选使用固定化氨基苯基硼酸），阴离子阳离子交换色谱法和阴性或阳性阴离子交换 色谱法。 还公开了一种用于降低白蛋白溶液中镍含量的方法，重组白蛋白编码序列还包含两个或更多个帧内翻译终止密码子。 还公开了一种生产重组白蛋白的方法，所述方法包括培养表达α的真菌细胞是重组白蛋白编码序列，其中所述细胞具有降低的重组表达白蛋白的甘露糖基化能力。
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公开(公告)号：US20110301329A1
公开(公告)日：2011-12-08
申请号：US13175155
申请日：2011-07-01
申请人： Hendrik Van Urk , David John Mead , Philip Harvey Morton , Andrew John Cartwright , Jason Cameron , David James Ballance , Michel Gaston Joseph Grandgeorge , Stephen Berezenko , John Rodney Woodrow , Darrell Sleep , Jean-Luc Bernard Veron
发明人： Hendrik Van Urk , David John Mead , Philip Harvey Morton , Andrew John Cartwright , Jason Cameron , David James Ballance , Michel Gaston Joseph Grandgeorge , Stephen Berezenko , John Rodney Woodrow , Darrell Sleep , Jean-Luc Bernard Veron
CPC分类号： B01D15/3804 , B01D15/362 , B01D15/363 , C07K14/76 , C07K14/765
摘要： A process is provided for the preparation of a highly pure albumin solution the process comprising subjecting albumin (preferably expressed and secreted by transformed yeast) to a series of chromatographic steps. Preferably, the process comprises the steps of positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilised aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography. A process for reducing the level of nickel in an albumin solution is also disclosed, as is a recombinant albumin coding sequence comprising two or more in-frame translation stop codons. Also disclosed is a process for producing recombinant albumin, the process comprising culturing a fungal cell expressing a is recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin.
摘要翻译： 提供了一种制备高纯度白蛋白溶液的方法，该方法包括使白蛋白（优选由转化酵母表达和分泌）经历一系列色谱步骤。 优选地，该方法包括以下步骤：阳离子阳离子交换层析，阳性阴离子交换层析，阳性模式亲和层析，负模式亲和色谱法（优选使用固定化氨基苯基硼酸），阴离子阳离子交换色谱法和阴性或阳性阴离子交换 色谱法。 还公开了一种用于降低白蛋白溶液中镍含量的方法，重组白蛋白编码序列还包含两个或更多个帧内翻译终止密码子。 还公开了一种生产重组白蛋白的方法，所述方法包括培养表达α的真菌细胞是重组白蛋白编码序列，其中所述细胞具有降低的重组表达白蛋白的甘露糖基化能力。
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公开(公告)号：US07993877B2
公开(公告)日：2011-08-09
申请号：US12207325
申请日：2008-09-09
申请人： Hendrik Van Urk , David John Mead , Philip Harvey Morton , Andrew John Cartwright , Jason Cameron , David James Ballance , Michel Gaston Joseph Grandgeorge , Stephen Berezenko , John Rodney Woodrow , Darrell Sleep , Jean-Luc Bernard Veron
发明人： Hendrik Van Urk , David John Mead , Philip Harvey Morton , Andrew John Cartwright , Jason Cameron , David James Ballance , Michel Gaston Joseph Grandgeorge , Stephen Berezenko , John Rodney Woodrow , Darrell Sleep , Jean-Luc Bernard Veron
CPC分类号： B01D15/3804 , B01D15/362 , B01D15/363 , C07K14/76 , C07K14/765
摘要： A process is provided for the preparation of a highly pure recombinant albumin solution having a nickel ion content of less than 100 ng per gram of albumin. The process comprises subjecting a recombinant albumin to a series of chromatography, concentration, and diafiltration steps.
摘要翻译： 提供了一种制备具有小于100ng /克白蛋白的镍离子含量的高纯度重组白蛋白溶液的方法。 该方法包括使重组白蛋白进行一系列色谱，浓缩和渗滤步骤。
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公开(公告)号：US20090171070A1
公开(公告)日：2009-07-02
申请号：US12207325
申请日：2008-09-09
申请人： Hendrik Van Urk , David John Mead , Philip Harvey Morton , Andrew John Cartwright , Jason Cameron , David James Ballance , Michel Gaston Joseph Grandgeorge , Stephen Berezenko , John Rodney Woodrow , Darrell Sleep , Jean-Luc Bernard Veron
发明人： Hendrik Van Urk , David John Mead , Philip Harvey Morton , Andrew John Cartwright , Jason Cameron , David James Ballance , Michel Gaston Joseph Grandgeorge , Stephen Berezenko , John Rodney Woodrow , Darrell Sleep , Jean-Luc Bernard Veron
IPC分类号： C07K14/765
CPC分类号： B01D15/3804 , B01D15/362 , B01D15/363 , C07K14/76 , C07K14/765
摘要： A process is provided for the preparation of a highly pure recombinant albumin solution having a nickel ion content of less than 100 ng per gram of albumin. The process comprises subjecting a recombinant albumin to a series of chromatography, concentration, and diafiltration steps.
摘要翻译： 提供了一种制备具有小于100ng /克白蛋白的镍离子含量的高纯度重组白蛋白溶液的方法。 该方法包括使重组白蛋白进行一系列色谱，浓缩和渗滤步骤。
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公开(公告)号：US20140234966A1
公开(公告)日：2014-08-21
申请号：US14130639
申请日：2012-07-05
IPC分类号： C12N5/0735 , C12N5/00
CPC分类号： C12N5/0018 , C07K14/435 , C07K14/76 , C12N5/0606 , C12N2500/30 , C12N2501/998
摘要： The invention relates to a new formulation of albumin, a method for producing the albumin formulation and to uses of the albumin formulation, for example in cell culture such as mammalian cell culture and particularly in stem cell culture.
摘要翻译： 本发明涉及白蛋白的新制剂，白蛋白制剂的制备方法和白蛋白制剂的用途，例如在细胞培养物如哺乳动物细胞培养物中，特别是在干细胞培养物中。
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