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公开(公告)号：US07678564B2
公开(公告)日：2010-03-16
申请号：US10505149
申请日：2003-02-20
CPC分类号： C12M35/02
摘要： The invention relates to a container 20, 30 for receiving an aqueous solution, which is formed at least partially by an outer limit 21 forming an inner chamber 22, 32 for receiving the solution, and which comprises at least one area which acts as an electrode 25, 26, 33, 34 when an electric voltage is applied and a subsequent discharge occurs, wherein at least one electrode 25, 26, 33, 34 is made of a conductive synthetic material at least based on a plastic material which is doped with at least one conductive substance. A container 20, 30 of the above-mentioned kind is created this way, which is simple and economical to produce and also, for example, enables an efficient transfection of living cells by means of electroporation or an effective electrofusion.
摘要翻译： 本发明涉及用于接收水溶液的容器20,30，其至少部分地由形成用于接收溶液的内室22,32的外限制21形成，并且包括至少一个充当电极的区域 25,26,33,34，当施加电压并发生随后的放电时，其中至少一个电极25,26,33,34由导电合成材料制成，至少基于掺杂有 至少一种导电物质。 以这种方式产生上述类型的容器20,30，其生产简单和经济，并且还例如能够通过电穿孔或有效的电融合有效转染活细胞。
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公开(公告)号：US07704727B2
公开(公告)日：2010-04-27
申请号：US10527958
申请日：2003-09-12
摘要： Disclosed is a device for processing biological material which at least comprises a chamber at least closable to the outside and having an inner space for receiving the biological material. The chamber comprises at least one electrode placed in contact with the inner space for generating an electric field. Also disclosed is a method for processing biological material. The biological material is introduced into the inner space above and the electrode can generate an electric field after said biological material is introduced by applying voltage to said electrode and a further electrode in contact with the inner space. The chamber comprises at least one inlet line having at least one opening arranged close to the electrode. The biological material is almost completely rinsed out of the inner space after the electric field is generated, via a solution guided via an inlet line of the chamber along at least one electrode.
摘要翻译： 公开了一种用于处理生物材料的装置，其至少包括至少可封闭到外部并且具有用于接收生物材料的内部空间的室。 该室包括至少一个与内部空间接触的电极，用于产生电场。 还公开了一种处理生物材料的方法。 将生物材料引入上述内部空间，并且通过向所述电极施加电压和与内部空间接触的另一电极而在引入所述生物材料之后，电极可以产生电场。 所述腔室包括至少一个入口管线，所述入口管线具有靠近所述电极设置的至少一个开口。 在通过沿着至少一个电极的腔室的入口管线引导的溶液后，生成电场后，生物材料几乎完全从内部空间中冲洗掉。
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3.发明授权公开(公告)号：US08173416B2
公开(公告)日：2012-05-08
申请号：US10475840
申请日：2002-04-23
申请人： Herbert Müller-Hartmann , Gudula Riemen , Kirsten Rothmann-Cosic , Corinna Thiel , Ludger Altrogge , Meike Weigel , Rainer Christine , Elke Lorbach , Juliana Helfrich , Heike Wessendorf , Gregor Siebenkotten
发明人： Herbert Müller-Hartmann , Gudula Riemen , Kirsten Rothmann-Cosic , Corinna Thiel , Ludger Altrogge , Meike Weigel , Rainer Christine , Elke Lorbach , Juliana Helfrich , Heike Wessendorf , Gregor Siebenkotten
CPC分类号： A61N1/327 , A61N1/0412 , C12N15/87 , H03K3/57
摘要： The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.
摘要翻译： 本发明涉及一种用于电转移或电融合的新型电路装置，其能够将DNA和/或其他生物活性分子运输到高等真核细胞的细胞核或细胞融合，而与细胞分裂无关并具有降低的细胞死亡率。
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4.发明授权Buffer solution for electroporation and a method comprising the use of the same 有权用于电穿孔的缓冲溶液和包括其使用的方法公开(公告)号：US07332332B2
公开(公告)日：2008-02-19
申请号：US10475967
申请日：2002-04-23
申请人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Müller-Hartmann , Kirsten Rothmann , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterbus , Michael Nix
发明人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Müller-Hartmann , Kirsten Rothmann , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterbus , Michael Nix
摘要： The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce biologically active molecules into cells using an electric current. The inventive buffer solution has a buffering capacity of at least 20 mmol−1×pH−1 and an ionic strength of at least 200 mmol×1−1 during a change to the pH value from pH 7 to pH 8 and at a temperature of 25° C. The use of a buffer solution of this type allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in the buffer solution.
摘要翻译： 本发明涉及用于悬浮动物或人细胞并溶解生物活性分子以便使用电流将生物活性分子引入细胞的缓冲溶液。 本发明的缓冲溶液具有至少20mmol -1pp-1的缓冲能力，并且离子强度至少为200mmol / 在pH值从pH7到pH8改变到25℃的温度下。使用这种缓冲溶液使生物活性分子以高转染效率被引入动物和人细胞中 同时细胞死亡率低。 不同的细胞类型，特别是休眠和活跃分裂细胞的低活性，可以成功地转染到缓冲溶液中。
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5.发明授权公开(公告)号：US08101401B2
公开(公告)日：2012-01-24
申请号：US11079192
申请日：2005-03-15
CPC分类号： C12M23/12 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00585 , B01J2219/00599 , B01J2219/00689 , B01J2219/00713 , B01J2219/00743 , B01L3/50855 , B01L2300/0645 , B01L2300/0829 , C12M35/02
摘要： The invention concerns a container 1 with chambers 2 which each comprise at least one pair of electrodes including a first 4 and a second electrode 5 for the application of electric voltage for generating an electric field within one chamber 2. At least two first electrodes 4 of different chambers 3 are conductively coupled and at least one second electrode 5 of said chamber 2 is separately conductively connectable. The invention further concerns a method for manufacturing said container 1 as well as a device for electrically contacting at least one of said containers 1.
摘要翻译： 本发明涉及具有腔室2的容器1，每个腔室2包括至少一对电极，其包括用于在一个腔室2内产生电场的电压施加的第一电极5和第二电极5.至少两个第一电极4 不同的腔室3被导电耦合，并且所述腔室2的至少一个第二电极5是可分开导电连接的。 本发明还涉及用于制造所述容器1的方法以及用于电接触所述容器1中的至少一个的装置。
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6.发明申请A METHOD AND A DEVICE FOR THE ELECTRICAL TREATMENT OF A PLURALITY OF CONTAINERS 有权一种用于电化学处理多种容器的方法和装置公开(公告)号：US20120067639A1
公开(公告)日：2012-03-22
申请号：US13145142
申请日：2010-01-20
CPC分类号： C12M35/02 , C12M23/44 , Y10T29/49117
摘要： The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device (1) for making electrical contact with at least one container fitted with electrodes, with at least one receptacle (3, 7) upon which at least one container can be set, and with at least one contact appliance (8) for making contact with the electrodes of the container. According to the invention at least two receptacles (3, 7) are provided, wherein one receptacle (7) is located at or inside the contact appliance (8), and wherein both receptacles (3, 7) and/or the contact appliance (8) can be moved such that after completion of the movement the other receptacle (3) is located at or inside the contact appliance (8).
摘要翻译： 本发明涉及一种用于将至少一个电压脉冲应用于装配有电极的至少两个容器的方法，通过该电容器将至少一个电压脉冲施加到至少一个容器，同时至少一个其它容器经受 准备或后期处理。 根据本发明，该方法包括相互更换已经施加了电压脉冲的容器的相应位置和另外的容器。 此外，本发明涉及一种用于与装有电极的至少一个容器进行电接触的装置（1），至少一个容器（3,7）可以在其上设置至少一个容器，并具有至少一个触点 器具（8），用于与容器的电极接触。 根据本发明，提供了至少两个插座（3,7），其中一个插座（7）位于接触器具（8）中或其内部，并且其中两个插座（3,7）和/或触头装置 8）可以移动，使得在移动完成之后，另一容器（3）位于联系设备（8）中或内部。
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公开(公告)号：US20120077245A1
公开(公告)日：2012-03-29
申请号：US13145113
申请日：2010-01-20
CPC分类号： C12M35/02
摘要： The invention relates to a method for applying at least one electrical voltage pulse to at least one reaction space by at least partially discharging at least one charge storing unit for storing electrical charges. According to the invention the method comprises applying at least one voltage pulse to a reaction space by at least partially discharging a second charge storing unit and simultaneously charging or partially discharging a first charge storing. Furthermore, the invention relates to a device 1 for applying at least one electrical voltage pulse to at least one reaction space, comprising at least two charge storing units (10, 11) for storing electrical charges and at least one power supply unit (12) for charging the charge storing units (10, 11) and/or at least one discharge device for partially discharging the charge storing units (10, 11). According to the invention it is provided that at least two of the charge storing units (10, 11) form a common storage module which can be discharged at any time.
摘要翻译： 本发明涉及通过至少部分地排放至少一个用于存储电荷的电荷存储单元来将至少一个电压脉冲施加到至少一个反应空间的方法。 根据本发明，该方法包括通过至少部分地排放第二电荷存储单元并同时对第一电荷存储进行充电或部分放电来将至少一个电压脉冲施加到反应空间。 此外，本发明涉及一种用于向至少一个反应空间施加至少一个电压脉冲的装置1，包括用于存储电荷的至少两个电荷存储单元（10,11）和至少一个电源单元（12） 用于对电荷存储单元（10,11）充电和/或用于部分地放电电荷存储单元（10,11）的至少一个放电装置。 根据本发明，提供了至少两个电荷存储单元（10,11）形成可以随时放电的公共存储模块。
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8.发明授权公开(公告)号：US09284525B2
公开(公告)日：2016-03-15
申请号：US13145142
申请日：2010-01-20
CPC分类号： C12M35/02 , C12M23/44 , Y10T29/49117
摘要： The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device (1) for making electrical contact with at least one container fitted with electrodes, with at least one receptacle (3, 7) upon which at least one container can be set, and with at least one contact appliance (8) for making contact with the electrodes of the container. According to the invention at least two receptacles (3, 7) are provided, wherein one receptacle (7) is located at or inside the contact appliance (8), and wherein both receptacles (3, 7) and/or the contact appliance (8) can be moved such that after completion of the movement the other receptacle (3) is located at or inside the contact appliance (8).
摘要翻译： 本发明涉及一种用于将至少一个电压脉冲应用于装配有电极的至少两个容器的方法，通过该电容器将至少一个电压脉冲施加到至少一个容器，同时至少一个其它容器经受 准备或后期处理。 根据本发明，该方法包括相互更换已经施加了电压脉冲的容器的相应位置和另外的容器。 此外，本发明涉及一种用于与装有电极的至少一个容器进行电接触的装置（1），其中至少一个容器（3,7）可以在其上设置至少一个容器，并具有至少一个触点 器具（8），用于与容器的电极接触。 根据本发明，提供了至少两个插座（3,7），其中一个插座（7）位于接触器具（8）中或其内部，并且其中两个插座（3,7）和/或触头装置 8）可以移动，使得在移动完成之后，另一容器（3）位于联系设备（8）中或内部。
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公开(公告)号：US09249384B2
公开(公告)日：2016-02-02
申请号：US13145113
申请日：2010-01-20
IPC分类号： C12M1/42
CPC分类号： C12M35/02
摘要： The invention relates to a method for applying at least one electrical voltage pulse to at least one reaction space by at least partially discharging at least one charge storing unit for storing electrical charges. According to the invention the method comprises applying at least one voltage pulse to a reaction space by at least partially discharging a second charge storing unit and simultaneously charging or partially discharging a first charge storing. Furthermore, the invention relates to a device 1 for applying at least one electrical voltage pulse to at least one reaction space, comprising at least two charge storing units (10, 11) for storing electrical charges and at least one power supply unit (12) for charging the charge storing units (10, 11) and/or at least one discharge device for partially discharging the charge storing units (10, 11). According to the invention it is provided that at least two of the charge storing units (10, 11) form a common storage module which can be discharged at any time.
摘要翻译： 本发明涉及通过至少部分地排放至少一个用于存储电荷的电荷存储单元来将至少一个电压脉冲施加到至少一个反应空间的方法。 根据本发明，该方法包括通过至少部分地排放第二电荷存储单元并同时对第一电荷存储进行充电或部分放电来将至少一个电压脉冲施加到反应空间。 此外，本发明涉及一种用于向至少一个反应空间施加至少一个电压脉冲的装置1，包括用于存储电荷的至少两个电荷存储单元（10,11）和至少一个电源单元（12） 用于对电荷存储单元（10,11）充电和/或用于部分地放电电荷存储单元（10,11）的至少一个放电装置。 根据本发明，提供至少两个电荷存储单元（10,11）形成可在任何时间放电的公共存储模块。
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