公开(公告)号：US20090291854A1

公开(公告)日：2009-11-26

申请号：US12307524

申请日：2007-07-05

申请人： Herbert Wiesinger-Mayr ,  Rudolf Pichler ,  Levente Bodrossy ,  Christa Nohammer

发明人： Herbert Wiesinger-Mayr ,  Rudolf Pichler ,  Levente Bodrossy ,  Christa Nohammer

IPC分类号： C40B30/02 ,  C40B30/04 ,  C40B40/08

CPC分类号： C12Q1/689 ,  C12Q1/6837 ,  C12Q1/6895 ,  C12Q2600/16 ,  G16B25/00

摘要： Disclosed is a method for identification of microbial pathogens in a body fluid sample comprising the following steps: a) providing a body fluid sample; b) lysing the microbial pathogens and performing a nucleic acid amplification reaction on the microbial DNA encoding 16S or 18S rRNA wherein or whereafter the amplified nucleic acids are labelled; c) contacting the labelled amplified nucleic acids of step b) with a microarray comprising on defined areas on the microarray's surface immobilised probes for microbial DNA encoding 16S or 18S rRNA from microbial pathogens; d) detecting the binding of one or more species of the labelled amplified nucleic acids to a probe by detecting a labelled amplified nucleic acid being specifically bound to the microarray; and e) identifying a microbial pathogen in the body fluid sample by correlating the detected binding of the labelled amplified nucleic acids with the defined areas of the immobilised probes for microbial DNA encoding 16S or 18S rRNA from microbial pathogens.

摘要翻译： 公开了一种用于鉴定体液样品中微生物病原体的方法，包括以下步骤：a）提供体液样品; b）裂解微生物病原体并对编码16S或18S rRNA的微生物DNA进行核酸扩增反应，其中扩增的核酸被标记; c）将步骤b）的标记的扩增的核酸与包含在微阵列表面上的限定区域上的微阵列接触，用于从微生物病原体编码16S或18S rRNA的微生物DNA的固定化探针; d）通过检测与微阵列特异性结合的标记的扩增核酸，检​​测一种或多种标记的扩增核酸与探针的结合; 和e）通过将检测到的标记的扩增的核酸的结合与来自微生物病原体的编码16S或18S rRNA的微生物DNA的固定化探针的限定区域相关联来鉴定体液样品中的微生物病原体。