Method of the solid phase synthesis of pyrrole-imidazole polyamide
    1.
    发明授权
    Method of the solid phase synthesis of pyrrole-imidazole polyamide 失效
    吡唑咪唑聚酰胺的固相合成方法

    公开(公告)号:US07067622B2

    公开(公告)日:2006-06-27

    申请号:US10481275

    申请日:2002-02-27

    IPC分类号: C07K1/02

    CPC分类号: C07D403/14

    摘要: It is intended to provide a method of producing a pyrrole-imidazole polyamide whereby a longer pyrrole-imidazole polyamide can be conveniently synthesized and a peptide (protein) can be easily transferred. According to this method, a pyrrole-imidazole polyamide having a carboxylate group which can be excised from a solid phase carrier at its end, makes it possible to directly transfer various functional groups and can exactly distinguish DNA sequences can be efficiently produced. A method of synthesizing a pyrrole-imidazole polyamide characterized by performing automatic synthesis by the solid phase Fmoc method with the used of a peptide synthesizer; a pyrrole-imidazole polyamide having a carboxyl group at its end obtained by this method; a pyrrole-imidazole polyamide having a DNA alkylation agent transferred into the carboxyl group at the end of the above-described pyrrole-imidazole polyamide; and a sequence-specific DNA alkylation method characterized by using the above compound.

    摘要翻译: 本发明提供一种制备吡咯 - 咪唑聚酰胺的方法,其中可以方便地合成较长的吡咯 - 咪唑聚酰胺,并且可以容易地转移肽(蛋白质)。 根据该方法,可以在其末端从固相载体上切割出具有羧酸酯基的吡咯 - 咪唑聚酰胺,从而可以直接转移各种官能团,并且能够精确地区分DNA序列。 一种合成吡咯 - 咪唑聚酰胺的方法,其特征在于使用肽合成仪通过固相Fmoc法进行自动合成; 通过该方法得到的具有羧基的吡咯 - 咪唑聚酰胺; 具有在上述吡咯 - 咪唑聚酰胺末端转移到羧基中的DNA烷基化剂的吡咯 - 咪唑聚酰胺; 特征在于使用上述化合物的序列特异性DNA烷基化方法。

    Apparatus for processing sheets and apparatus for forming images provided with the same

    公开(公告)号:US10259673B2

    公开(公告)日:2019-04-16

    申请号:US15883990

    申请日:2018-01-30

    摘要: A sheet processing apparatus includes a transport path to transport a sheet to a first tray, a branch path branched off from the transport path to transport a sheet to a second tray, a first transport roller positioned in the transport path on a downstream side of a branch position of the transport path and the branch path to transport a sheet in either direction of the first tray or the branch path, a second transport roller positioned on the branch path to transport a sheet in either direction of the second tray or the transport path, and a control section recognizing a transport length of the sheet transported by the first transport roller and the second transport roller, and controlling the first transport roller and the second transport roller. The control section performs first and second transports for switchback-transporting a sheet.