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公开(公告)号：US06939682B2
公开(公告)日：2005-09-06
申请号：US10415726
申请日：2001-11-07
摘要： The present invention relates to a test piece having a simple structure, by which HDL cholesterol can be measured easily using a small amount of specimen. A reagent layer (2) is formed on a support (1), and an enzyme reagent for measuring cholesterol, a first surfactant that makes a solubility of a high-density lipoprotein (HDL) higher than that of a lipoprotein other than the HDL, and a second surfactant that inhibits the lipoprotein other than the HDL from dissolving are contained in the reagent layer (2).
摘要翻译： 本发明涉及具有简单结构的测试片，通过该试片可以使用少量试样容易地测量HDL胆固醇。 在载体（1）上形成试剂层（2），测定胆固醇用的酶试剂，高密度脂蛋白（HDL）的溶解度比HDL以外的脂蛋白高的第一表面活性剂， 并且在试剂层（2）中含有抑制HDL以外的脂蛋白溶解的第二表面活性剂。
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2.发明授权公开(公告)号：US07575884B2
公开(公告)日：2009-08-18
申请号：US11184117
申请日：2005-07-19
摘要： The present invention relates to a test piece having a simple structure, by which HDL cholesterol can be measured easily using a small amount of specimen. A reagent layer (2) is formed on a support (1), and an enzyme reagent for measuring cholesterol, a first surfactant that makes a solubility of a high-density lipoprotein (HDL) higher than that of a lipoprotein other than the HDL, and a second surfactant that inhibits the lipoprotein other than the HDL from dissolving are contained in the reagent layer (2).
摘要翻译： 本发明涉及具有简单结构的测试片，通过该试片可以使用少量试样容易地测量HDL胆固醇。 在载体（1）上形成试剂层（2），测定胆固醇用的酶试剂，高密度脂蛋白（HDL）的溶解度比HDL以外的脂蛋白高的第一表面活性剂， 并且在试剂层（2）中含有抑制HDL以外的脂蛋白溶解的第二表面活性剂。
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3.发明申请公开(公告)号：US20050250165A1
公开(公告)日：2005-11-10
申请号：US11184117
申请日：2005-07-19
摘要： The present invention relates to a test piece having a simple structure, by which HDL cholesterol can be measured easily using a small amount of specimen. A reagent layer (2) is formed on a support (1), and an enzyme reagent for measuring cholesterol, a first surfactant that makes a solubility of a high-density lipoprotein (HDL) higher than that of a lipoprotein other than the HDL, and a second surfactant that inhibits the lipoprotein other than the HDL from dissolving are contained in the reagent layer (2).
摘要翻译： 本发明涉及具有简单结构的测试片，通过该试片可以使用少量试样容易地测量HDL胆固醇。 在载体（1）上形成试剂层（2），测定胆固醇用的酶试剂，高密度脂蛋白（HDL）的溶解度比HDL以外的脂蛋白高的第一表面活性剂， 并且在试剂层（2）中含有抑制HDL以外的脂蛋白溶解的第二表面活性剂。
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公开(公告)号：US20060183179A1
公开(公告)日：2006-08-17
申请号：US11399447
申请日：2006-04-07
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
摘要翻译： 一种定量测定LDL胆固醇的方法，包括以下步骤：向血清中加入选自聚氧乙烯亚烷基苯基醚和聚氧乙烯亚烷基三苄基苯基醚的表面活性剂和胆固醇分析酶试剂，以优先反应高密度和非常低密度的胆固醇中的胆石甾醇 在脂蛋白中，然后确定其后反应的胆固醇的量。 该方法可以消除离心和电泳等预处理的必要性，能够以高效，简单的方式进行定量测定，可以应用于各种自动分析装置。
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公开(公告)号：US5773304A
公开(公告)日：1998-06-30
申请号：US704681
申请日：1996-09-19
CPC分类号： G01N33/92 , C12Q1/60 , Y10S435/962 , Y10S436/815 , Y10S436/824 , Y10S436/825 , Y10S436/826 , Y10T436/104165 , Y10T436/107497 , Y10T436/25
摘要： A method for quantitatively determining cholesterol in high density lipoproteins, in which, prior to the determination of cholesterol by an enzymatic method, a surfactant and a substance which forms a complex with lipoproteins other than high density lipoproteins are added to a sample containing lipoproteins. The method does not require any pretreatments such as centrifugal separation. With a simple operation, cholesterol in HDLs can be measured effectively. Also, this method can be adopted in a variety of automated analyzers, and thus is very useful in the field of clinical assays.
摘要翻译： PCT No.PCT / JP95 / 00641 Sec。 371日期1996年9月19日 102（e）1996年9月19日PCT PCT 1995年4月3日PCT公布。 WO96 / 23902 PCT公开号 日期1996年8月8日一种用于定量测定高密度脂蛋白胆固醇的方法，其中在通过酶法测定胆固醇之前，将表面活性剂和与高密度脂蛋白以外的脂蛋白形成复合物的物质加入到 样品含有脂蛋白。 该方法不需要任何预处理，如离心分离。 通过简单的操作，可以有效测量HDL中的胆固醇。 此外，该方法可以在各种自动分析仪中使用，因此在临床测定领域非常有用。
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公开(公告)号：US20080131911A1
公开(公告)日：2008-06-05
申请号：US12025369
申请日：2008-02-04
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholesterols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
摘要翻译： 一种定量测定LDL胆固醇的方法，包括以下步骤：向血清中加入选自聚氧乙烯亚烷基苯基醚和聚氧乙烯亚烷基三苄基苯基醚的表面活性剂和胆固醇分析酶试剂，以便优先反应高密度和非常低密度的胆固醇中的胆固醇 在脂蛋白中，然后确定其后反应的胆固醇的量。 该方法可以消除离心和电泳等预处理的必要性，能够以高效，简单的方式进行定量测定，可以应用于各种自动分析装置。
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公开(公告)号：US06764828B2
公开(公告)日：2004-07-20
申请号：US09971673
申请日：2001-10-09
IPC分类号： C12Q160
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
摘要翻译： 一种定量测定LDL胆固醇的方法，包括以下步骤：向血清中加入选自聚氧乙烯亚烷基苯基醚和聚氧乙烯亚烷基三苄基苯基醚的表面活性剂和胆固醇分析酶试剂，以优先反应高密度和非常低密度的胆固醇中的胆石甾醇 在脂蛋白中，然后确定其后反应的胆固醇的量。 该方法可以消除离心和电泳等预处理的必要性，能够以高效，简单的方式进行定量测定，可以应用于各种自动分析装置。
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公开(公告)号：US20120149047A1
公开(公告)日：2012-06-14
申请号：US13179928
申请日：2011-07-11
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
摘要翻译： 一种定量测定LDL胆固醇的方法，包括以下步骤：向血清中加入选自聚氧乙烯亚烷基苯基醚和聚氧乙烯亚烷基三苄基苯基醚的表面活性剂和胆固醇分析酶试剂，以优先反应高密度和非常低密度的胆固醇中的胆石甾醇 在脂蛋白中，然后确定其后反应的胆固醇的量。 该方法可以消除离心和电泳等预处理的必要性，能够以高效，简单的方式进行定量测定，可以应用于各种自动分析装置。
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公开(公告)号：US20100227309A1
公开(公告)日：2010-09-09
申请号：US12782447
申请日：2010-05-18
IPC分类号： C12Q1/00
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
摘要翻译： 一种定量测定LDL胆固醇的方法，包括以下步骤：向血清中加入选自聚氧乙烯亚烷基苯基醚和聚氧乙烯亚烷基三苄基苯基醚的表面活性剂和胆固醇分析酶试剂，以优先反应高密度和非常低密度的胆固醇中的胆石甾醇 在脂蛋白中，然后确定其后反应的胆固醇的量。 该方法可以消除离心和电泳等预处理的必要性，能够以高效，简单的方式进行定量测定，可以应用于各种自动分析装置。
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公开(公告)号：US6057118A
公开(公告)日：2000-05-02
申请号：US147296
申请日：1998-11-23
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
摘要翻译： PCT No.PCT / JP97 / 01232 Sec。 371日期：1998年11月23日 102（e）1998年11月23日PCT PCT 1997年4月10日PCT公布。 公开号WO97 / 45553 日期1997年12月4日定量测定LDL胆固醇的方法，包括向血清中加入选自聚氧乙烯亚烷基苯基醚和聚氧亚乙基亚烷基三苄基苯基醚的表面活性剂和胆固醇分析酶试剂，以优先反应高密度胆固醇， 脂蛋白中的非常低密度的胆固醇，随后测定其后反应的胆固醇的量。 该方法可以消除离心和电泳等预处理的必要性，能够以高效，简单的方式进行定量测定，可以应用于各种自动分析装置。
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