公开(公告)号：US20070134783A1

公开(公告)日：2007-06-14

申请号：US10594899

申请日：2005-03-30

申请人： Hirotaka Kakita ,  Hiroshi Kamishima

发明人： Hirotaka Kakita ,  Hiroshi Kamishima

IPC分类号： C12N1/12

CPC分类号： A01H13/00

摘要： It is intended to provide a novel unialgal culture strain showing a high culture efficiency of a large-sized red alga which is immature, can be stored and cultured over a long period of time and has at least one of the following properties, i.e., producing a physiologically active substance at a high yield, showing a high growth speed of the alga body, and being highly capable of absorbing nutritional salts. Namely, an immature unialgal culture strain originating in a large-sized red marine alga which is characterized by showing no matured female gametophyte in nature but showing matured tetrasporophyte alone and grows in a natural marine water area containing fresh water. This unialgal culture strain is constructed by collecting the matured sporophyte, cutting the sporophyte and allowing to stand to thereby release spores, culturing the released spores and continuously proliferating and culturing even after the growth of an upright body from the germinated spore.

摘要翻译： 旨在提供一种显示高度培养效率的新型单一藻类培养菌株，它是不成熟的，可以长时间储存​​和培养，并且具有至少一种以下性质，即生产 高产量的生理活性物质，显示出藻体的高生长速度，并且能够高吸收营养盐。 即来自大型红色海洋藻类的未成熟的非海藻培养物种，其特征在于本身没有显现成熟的雌配子体，但是在单独存在成熟的四孢子虫并且在含有淡水的天然海水区域中生长。 通过收集成熟的孢子体，切割孢子体并允许静置以释放孢子，培养释放的孢子并且即使在从发芽孢子生长直立体之后也继续增殖和培养，构建这种单性培养菌株。

公开(公告)号：US20060078569A1

公开(公告)日：2006-04-13

申请号：US11241926

申请日：2005-10-04

申请人： Hirotaka Kakita ,  Hiroshi Kamishima

发明人： Hirotaka Kakita ,  Hiroshi Kamishima

IPC分类号： A61K36/02

CPC分类号： A61K36/04

摘要： The present invention provides a novel method for recovery of photo-inhibited immunocompetence, which comprises administration of a specific active ingredient exhibiting physiological activity such as activity of activating cellular immunocompetence, which is obtained from a raw material that exists in a large amount in the nature and can easily be acquired. The above active ingredient is obtained in the form of a liquid crude active fraction by: adding ammonium sulfate to an extract obtained from Gracilaria sp., using an aqueous salt solution, to a final concentration of 20% to 40% saturated solution to conduct a first stage of salting-out, so as to eliminate the precipitated contaminants; further adding ammonium sulfate to the extract to a final concentration of 60% to 80% saturated solution to conduct a second stage of salting-out, so as to recover a crude active fraction as a precipitate; and dissolving the precipitate in a suitable solvent. It is also obtained by dissolving the above precipitate in a buffer solution, allowing the obtained solution to come into contact with a dialyzing fluid that has been adjusted to the isoelectric point of a substance exhibiting physiological activity, via a dialysis membrane, so as to transfer low molecular weight impurities to the dialyzing fluid and eliminate them, and at the same time, precipitating a physiologically active polymer from a solution containing high molecular weight impurities and recovering it.

摘要翻译： 本发明提供了一种新的光抑制免疫能力的回收方法，该方法包括给予表现出活性的活性成分的特定活性成分，所述活性成分是从大量存在的大量原料中获得的激活细胞免疫能力的活性 并可以轻松获得。 通过以下方式获得上述活性成分：通过以下方法获得液态粗活性成分：通过使用盐水溶液向从江ia提取物中提取硫酸铵至最终浓度为20％至40％的饱和溶液以进行 第一阶段盐析，以消除沉淀的污染物; 进一步向提取物中加入硫酸铵至最终浓度为60％至80％饱和溶液，进行第二级盐析，以回收粗活性级分作为沉淀物; 并将沉淀物溶解在合适的溶剂中。 也可以通过将上述沉淀物溶解在缓冲溶液中，通过透析膜将得到的溶液与已经调整为具有生理活性的物质的等电点的透析液接触，从而转移 低分子量杂质转移到透析液中并消除它们，同时从含有高分子量杂质的溶液中沉淀生理活性聚合物并回收它。

公开(公告)号：US20070029259A1

公开(公告)日：2007-02-08

申请号：US10543530

申请日：2004-02-04

申请人： Hirotaka Kakita ,  Akinari Sonoda ,  Kazutoshi Yoshihara ,  Hiroshi Kamishima ,  Takahiro Hirotsu ,  Kenta Ooi ,  Kazuyuki Takashima ,  Tetuo Iwasaki ,  Eiji Santoki

发明人： Hirotaka Kakita ,  Akinari Sonoda ,  Kazutoshi Yoshihara ,  Hiroshi Kamishima ,  Takahiro Hirotsu ,  Kenta Ooi ,  Kazuyuki Takashima ,  Tetuo Iwasaki ,  Eiji Santoki

IPC分类号： C02F1/42

CPC分类号： A01G33/00 ,  B01D15/00 ,  B01D15/265 ,  C01D3/16 ,  Y02A40/88

摘要： A method in which an aqueous water-soluble compound solution containing at least one impurity in a slight amount is passed through a column packed with an adsorbent onto which the impurity is selectively adsorbed to thereby remove the impurity from the aqueous solution, wherein an abnormal phenomenon in adsorption chromatography is caused to obtain an elute fraction having a higher concentration of the trace impurity than the raw solution and this fraction is removed. Thus, the trace impurity can be efficiently removed and the water-soluble compound can be obtained in a high-purity state. Applying this method to aqueous sodium chloride solutions gives sodium chloride crystals reduced in impurity concentration. Dissolving such sodium chloride crystals, which have a low potassium ion concentration, in water provides an aqueous sodium chloride solution for medical use. Also provided is a sodium chloride composition for preparing an artificial seawater for use in alga cultivation which is reduced in magnesium ion or calcium ion concentration.

摘要翻译： 将含有少量杂质的含水溶性化合物溶液通过填充有选择性吸附有杂质的吸附剂的柱，从而从水溶液中除去杂质的方法，其中异常现象 使吸附色谱法得到具有比原始溶液高的痕量杂质浓度的洗脱级分，并除去该级分。 因此，可以有效地除去痕量杂质，并且可以以高纯度的状态获得水溶性化合物。 将该方法应用于氯化钠水溶液中使得氯化钠晶体的杂质浓度降低。 将具有低钾离子浓度的这种氯化钠晶体溶解在水中，提供用于医疗用途的氯化钠水溶液。 还提供了用于制备用于藻类培养的人造海水的氯化钠组合物，其降低了镁离子或钙离子浓度。

公开(公告)号：US06855555B2

公开(公告)日：2005-02-15

申请号：US10327125

申请日：2002-12-24

申请人： Hirotaka Kakita ,  Hiroshi Kamishima

发明人： Hirotaka Kakita ,  Hiroshi Kamishima

IPC分类号： G01N21/15 ,  B01J20/283 ,  G01N30/26 ,  G01N30/74 ,  G01N30/84 ,  G01N30/88 ,  G01N30/38

CPC分类号： G01N30/84 ,  G01N2030/8435 ,  Y10T436/143333 ,  Y10T436/144444

摘要： In conducting liquid chromatographic analysis of a saccharide mixture containing monosaccharides and oligosaccharides, elution is conducted by using two kinds or more of mobile phases to separate the saccharide mixture into individual constituent saccharides followed by conversion thereof into corresponding derivatives by reaction with reagents and detection of the derivatives as contained in a detector cell. The method of the invention is characterized in that the detector cell is cleaned by washing with a cleaning solvent after completion of the detection of each of the saccharide derivatives. The invention also provides an apparatus for efficiently conducting the above-mentioned inventive method comprising an analytical column, a reactor for converting the separated constituent saccharides into the derivatives, detector cell for containing the derivative, a detector for detecting the derivative contained in the detector cell, a solvent-feed means to introduce a cell-cleaning solvent into the cell and a flow channel-switching means.

摘要翻译： 在对含有单糖和寡糖的糖类混合物进行液相色谱分析时，通过使用两种以上的流动相进行洗脱，将糖类混合物分离为各成分糖类，然后通过与试剂反应转化成相应的衍生物， 衍生物包含在检测器单元中。 本发明的方法的特征在于，检测单元在完成每种糖衍生物的检测后用清洗溶剂进行清洗。 本发明还提供了一种用于有效地进行上述本发明方法的装置，包括分析柱，用于将分离的组成糖转化为衍生物的反应器，用于包含衍生物的检测器单元，用于检测检测器单元中所含的衍生物的检测器 ，将细胞清洗溶剂引入细胞的溶剂进料装置和流路切换装置。