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公开(公告)号：US20110229980A1
公开(公告)日：2011-09-22
申请号：US12982931
申请日：2010-12-31
申请人： Hiroyuki ASANUMA , Xingguo Liang , Hiromu Kashida , Yasuko Yoshida , Tomokazu Takase , Kousuke Niwa
发明人： Hiroyuki ASANUMA , Xingguo Liang , Hiromu Kashida , Yasuko Yoshida , Tomokazu Takase , Kousuke Niwa
CPC分类号： C12Q1/6816 , C12Q2525/301 , C12Q2565/501
摘要： The present teaching provides a fluorescent oligonucleotide probe having a high degree of design flexibility and wide applicability, as well as the use thereof. This is an oligonucleotide probe capable of forming a stem and loop, comprising at least one fluorophore located between adjacent nucleotides in the stem and is linked to a unit represented by Formula (1) and at least one quencher located at a site capable of pairing up with the at least one fluorophore located between the adjacent nucleotides in the stem and is linked to a unit represented by Formula (2). (In the formulae, X represents the fluorophore, Y represents the quencher, R1 represents an optionally substituted C2 or C3 alkylene chain, R2 represents an optionally substituted C0-2 alkylene chain, and Z represents a direct bond or linker.)
摘要翻译： 本教导提供具有高设计灵活性和广泛适用性的荧光寡核苷酸探针及其用途。 这是能够形成茎和环的寡核苷酸探针，其包含位于茎中相邻核苷酸之间的至少一个荧光团，并连接到由式（1）表示的单元和位于能够配对的位点处的至少一个猝灭剂 其中所述至少一个荧光团位于茎中相邻核苷酸之间，并与由式（2）表示的单元连接。 （式中，X表示荧光团，Y表示猝灭剂，R 1表示任选取代的C 2或C 3亚烷基链，R 2表示任选取代的C 0-2亚烷基链，Z表示直接键或连接基。
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公开(公告)号：US09018362B2
公开(公告)日：2015-04-28
申请号：US13819954
申请日：2011-07-22
申请人： Hiroyuki Asanuma , Xingguo Liang , Hiromu Kashida , Hidenori Nishioka , Taiga Fujii , Teruchika Ishikawa
发明人： Hiroyuki Asanuma , Xingguo Liang , Hiromu Kashida , Hidenori Nishioka , Taiga Fujii , Teruchika Ishikawa
摘要： Provided is an oligonucleotide containing an azobenzene derivative, represented by Formula (1) or (2) below: (in the formulae, A1 and A2 each independently represent a hydrogen atom, nucleotide or oligonucleotide, B1 and B2 each independently represent a hydroxyl group, nucleotide or oligonucleotide, R11 and R12 each independently represent a C1-20 alkyl group, R21 and R22 each independently represent a hydrogen atom or C1-20 alkyl group, and R13 to R18 and R23 to R28 each independently represent a hydrogen atom; a C1-20 alkyl group or alkoxy group optionally substituted with a halogen atom, hydroxyl group, amino group, nitro group or carboxyl group; a C2-20 alkenyl group or alkynyl group optionally substituted with a halogen atom, hydroxyl group, amino group, nitro group or carboxyl group; a hydroxyl group; a halogen atom; an amino group; a nitro group; or a carboxyl group).
摘要翻译： 本发明提供含有式（1）或（2）表示的偶氮苯衍生物的寡核苷酸：（式中，A1和A2各自独立地表示氢原子，核苷酸或寡核苷酸，B1和B2各自独立地表示羟基， 核苷酸或寡核苷酸，R 11和R 12各自独立地表示C 1-20烷基，R 21和R 22各自独立地表示氢原子或C 1-20烷基，R 13〜R 18和R 23〜R 28各自独立地表示氢原子; C1 -20任选被卤素原子，羟基，氨基，硝基或羧基取代的烷基或烷氧基;任选被卤素原子取代的C 2-20烯基或炔基，羟基，氨基，硝基 或羧基;羟基;卤素原子;氨基，硝基或羧基）。
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公开(公告)号：US20130225797A1
公开(公告)日：2013-08-29
申请号：US13819954
申请日：2011-07-22
申请人： Hiroyuki Asanuma , Xingguo Liang , Hiromu Kashida , Hidenori Nishioka , Taiga Fujii , Teruchika Ishikawa
发明人： Hiroyuki Asanuma , Xingguo Liang , Hiromu Kashida , Hidenori Nishioka , Taiga Fujii , Teruchika Ishikawa
摘要： Provided is an oligonucleotide containing an azobenzene derivative, represented by Formula (1) or (2) below: (in the formulae, A1 and A2 each independently represent a hydrogen atom, nucleotide or oligonucleotide, B1 and B2 each independently represent a hydroxyl group, nucleotide or oligonucleotide, R11 and R12 each independently represent a C1-20 alkyl group, R21 and R22 each independently represent a hydrogen atom or C1-20 alkyl group, and R13 to R18 and R23 to R28 each independently represent a hydrogen atom; a C1-20 alkyl group or alkoxy group optionally substituted with a halogen atom, hydroxyl group, amino group, nitro group or carboxyl group; a C2-20 alkenyl group or alkynyl group optionally substituted with a halogen atom, hydroxyl group, amino group, nitro group or carboxyl group; a hydroxyl group; a halogen atom; an amino group; a nitro group; or a carboxyl group).
摘要翻译： 本发明提供含有式（1）或（2）表示的偶氮苯衍生物的寡核苷酸：（式中，A1和A2各自独立地表示氢原子，核苷酸或寡核苷酸，B1和B2各自独立地表示羟基， 核苷酸或寡核苷酸，R 11和R 12各自独立地表示C 1-20烷基，R 21和R 22各自独立地表示氢原子或C 1-20烷基，R 13〜R 18和R 23〜R 28各自独立地表示氢原子; C1 -20任选被卤素原子，羟基，氨基，硝基或羧基取代的烷基或烷氧基;任选被卤素原子取代的C 2-20烯基或炔基，羟基，氨基，硝基 或羧基;羟基;卤素原子;氨基，硝基或羧基）。
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公开(公告)号：US20130284944A1
公开(公告)日：2013-10-31
申请号：US13580677
申请日：2011-02-28
CPC分类号： H01B3/18 , C09B3/14 , C09B5/62 , C09B11/24 , C12Q1/6818 , G01N21/64 , G01N2021/6432
摘要： An object of the present invention is to provide an insulator which can suppress quenching between fluorescent dyes and enhance fluorescence intensity. The present invention provides, in order to achieve this object, an insulator which contains a ring entity of nonplanar structure and suppresses reduction in fluorescence intensity of one or two or more fluorescent labels adjacent to the insulator.
摘要翻译： 本发明的目的是提供一种可以抑制荧光染料之间淬灭并增强荧光强度的绝缘体。 为了实现该目的，本发明提供了一种绝缘子，其包含非平面结构的环形实体并且抑制与绝缘体相邻的一个或两个或更多个荧光标签的荧光强度的降低。
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