公开(公告)号：US08254019B2

公开(公告)日：2012-08-28

申请号：US12585888

申请日：2009-09-28

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/00

CPC分类号： G02B21/008 ,  G02B21/0032

摘要： The present application generates an image suitable for collectively observing a whole area or all wavelength components in a viewing field. A confocal microscope apparatus includes a detecting unit being disposed on a collecting location of the collecting optical system, separating incident light into a light from a vicinity of a collecting point on the sample and a light from a peripheral of the vicinity, and detecting each of the lights; and an image generating unit generating an image of the sample by a light signal from the vicinity and a light signal from the peripheral of the vicinity, and setting a ratio of the signal of the light from the vicinity of the collecting point to the signal of the light from the peripheral of the vicinity for each of areas on the image.

摘要翻译： 本申请生成适合于在观察区域中共同观察整个区域或全部波长成分的图像。 共聚焦显微镜装置包括：检测单元，设置在收集光学系统的收集位置，将入射光分离成来自样品上的收集点附近的光和来自周围的周边的光; 灯光; 以及图像生成单元，其通过来自附近的光信号和来自周围的周边的光信号生成样本的图像，并且将来自收集点附近的光的信号的比率设定为 来自图像上每个区域的附近的周边的光。

公开(公告)号：US07639357B2

公开(公告)日：2009-12-29

申请号：US12318014

申请日：2008-12-19

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G01N21/64 ,  G01J3/30

CPC分类号： G02B21/0076 ,  G01N21/6458 ,  G02B21/0032 ,  G02B21/0048 ,  G02B21/0064 ,  G02B21/32

摘要： To provide a laser scanning microscope capable of enhancing the degree of freedom of observation while keeping its structure simple. Accordingly, a laser scanning microscope includes a light source, a spectroscopic unit guiding light from the light source to a specimen and guiding the light from the specimen to a detector, light path switching units switching a light path between the spectroscopic unit and the specimen to one among a plurality of light paths with different routes, and a plurality of light deflecting units each disposed in each of the plurality of light paths.

摘要翻译： 提供能够提高观察自由度的激光扫描显微镜，同时保持其结构简单。 因此，激光扫描显微镜包括光源，分光单元，其将来自光源的光引导到样本并将来自样本的光引导到检测器，将光谱单元和样本之间的光路切换到 具有不同路线的多个光路中的一个，以及分别设置在多个光路中的每一个中的多个光偏转单元。

公开(公告)号：US20090128806A1

公开(公告)日：2009-05-21

申请号：US11913294

申请日：2007-02-01

申请人： Masafumi Mimura ,  Hisashi Okugawa

发明人： Masafumi Mimura ,  Hisashi Okugawa

IPC分类号： G01J3/30

CPC分类号： G01N21/6458 ,  G01N2021/6417 ,  G01N2021/6441

摘要： A spectral image processing system and method of performing robust unmixing on measurement noise. Based on an observed spectral image acquired from a specimen and emission spectral data of each of plural materials contained in the specimen, a contribution of each of the plural materials to the observed spectral image is unmixed by a process, including an evaluating step of evaluating reliability of each component of the observed spectral image based on a predicted spectral image of the observed spectral image, and a reflecting step of reflecting a result of the evaluation in a content of the unmixing.

摘要翻译： 一种对测量噪声进行稳健解混的光谱图像处理系统和方法。 基于从样本获取的观察到的光谱图像和包含在样本中的多种材料的发射光谱数据，通过包括评价可靠性的评估步骤的处理，将多种材料中的每一种对所观察的光谱图像的贡献未混合 基于观测到的光谱图像的预测光谱图像，以及将评价结果反映在解混合物的内容中的反射步骤。

公开(公告)号：US20050213206A1

公开(公告)日：2005-09-29

申请号：US11081731

申请日：2005-03-17

申请人： Hisashi Okugawa ,  Naoshi Aikawa

发明人： Hisashi Okugawa ,  Naoshi Aikawa

IPC分类号： G01B11/14 ,  G01B11/24 ,  G02B21/00 ,  G02B21/06

CPC分类号： G02B21/0076 ,  G02B21/0044 ,  G02B21/008

摘要： A confocal microscope includes a light source that emits illuminating light beam, an illuminating optical system that irradiates the illuminating light beam onto a specimen, a condensing optical system that condenses the light reflected off the specimen and a light detection unit. The light detection unit includes a mask member, a movable shutter and a light detector. The mask member includes a plurality of reflecting/transmitting surfaces each constituting a light separating surface, and only the central portion of the light flux entering the mask member from the condensing optical system exits through a pinhole. The movable shutter opens or closes off a plurality of pinholes individually, and the light detector detects the light flux having passed through the pinholes.

摘要翻译： 共焦显微镜包括发射照明光束的光源，将照射光束照射到样本上的照明光学系统，冷凝从样本反射的光的聚光光学系统和光检测单元。 光检测单元包括掩模构件，可移动遮板和光检测器。 掩模构件包括多个反射/透射表面，每个反射/透射表面均构成光分离表面，并且只有从聚光系统进入掩模构件的光束的中心部分通过针孔排出。 可移动快门单独地打开或关闭多个针孔，并且光检测器检测穿过针孔的光通量。

公开(公告)号：US20100020392A1

公开(公告)日：2010-01-28

申请号：US12585890

申请日：2009-09-28

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/06

CPC分类号： G02B21/008

摘要： The present application detects a state change of an object in an optical axis direction by a single frame scanning for each round. A confocal microscope apparatus includes a detecting unit separating incident light into a light from a vicinity of a collecting point on the sample and a light from a peripheral of the vicinity and detecting each of the lights, and an image generating unit generating an image of the sample by a light signal from the vicinity and a light signal from the peripheral, in which a distinction between a light signal from the vicinity and a light signal from the peripheral is calculated for each of a plurality of images generated in a number of times of scanning without changing a collecting location in an optical axis direction.

摘要翻译： 本申请通过每轮的单帧扫描来检测物体在光轴方向上的状态变化。 共聚焦显微镜装置包括检测单元，其将入射光分离成来自样品上的收集点附近的光和来自附近的周边的光并检测每个光;以及图像生成单元，生成图像的图像 通过来自附近的光信号进行采样以及来自外围设备的光信号，其中计算来自附近的光信号与来自外围设备的光信号之间的区别，其中对于多次产生的多个图像中的每一个， 扫描而不改变光轴方向的收集位置。

公开(公告)号：US20090080722A1

公开(公告)日：2009-03-26

申请号：US11913281

申请日：2007-02-01

申请人： Hisashi Okugawa ,  Masafumi Mimura

发明人： Hisashi Okugawa ,  Masafumi Mimura

IPC分类号： G06K9/00

CPC分类号： G01N21/274 ,  G01N21/6458 ,  G06T5/002 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30024 ,  H04N1/409

摘要： A spectral image processing method is capable of reducing noise while maintaining necessary information. The spectral image processing method performs processing on a spectral image of a specimen, including a step of normalizing spectra (=spectral brightness curves) of respective pixels constituting the spectral image such that their brightness levels become equal, a step of smoothing the normalized spectra in spatial directions of the respective pixels, and a step of denormalization of multiplying spectra of the respective pixels obtained by the smoothing by either one of brightness levels of the pixels corresponding the spectra and values corresponding to the brightness levels. Consequently, the noise can be reduced while information on brightness distribution on the image is maintained.

摘要翻译： 光谱图像处理方法能够在保持必要信息的同时降低噪声。 光谱图像处理方法对样本的光谱图像执行处理，包括使构成光谱图像的各个像素的光谱（=光谱亮​​度曲线）归一化为使得它们的亮度水平变得相等的步骤，使平均化的归一化光谱的步骤 各个像素的空间方向，以及通过平滑获得的各个像素的乘积光谱的非归一化的步骤，对应于对应于亮度等级的光谱和值的像素的亮度级中的任一个。 因此，可以降低噪声，同时保持图像上亮度分布的信息。

公开(公告)号：US07330257B2

公开(公告)日：2008-02-12

申请号：US11207872

申请日：2005-08-22

申请人： Yoshinori Kuroiwa ,  Hisashi Okugawa

发明人： Yoshinori Kuroiwa ,  Hisashi Okugawa

IPC分类号： G01J3/28

CPC分类号： G01N21/6458 ,  G01J3/02 ,  G01J3/0237 ,  G01J3/28 ,  G01J3/2803 ,  G01J3/4406 ,  G01N2021/6421

摘要： An object is to provide a spectroscope and a spectrum laser microscope capable of carrying out sensitivity correction of a multi-channel photodetector with real time. The spectrum laser microscope 100 includes a laser microscope 101 and a spectral analyzer 103 having a multi-channel photodetector 13 composed of a plurality of photodetectors 13i for detecting spectral distribution of the light from the laser microscope 101. Sensitivity fluctuation of the plurality of photodetectors 13i is calculated from a first luminance data detected before shifting relative position between the spectra and the multi-channel photodetector 13 and a second luminance data detected after shifting. Then, the first luminance data or the second luminance data is corrected.

摘要翻译： 目的是提供能够实时地进行多通道光电检测器的灵敏度校正的分光计和光谱激光显微镜。 光谱激光显微镜100包括激光显微镜101和具有由用于检测来自激光显微镜101的光的光谱分布的多个光电检测器13i组成的多通道光电检测器13的光谱分析器103。 根据在频谱和多声道光电探测器13之间的相对位置移动之前检测到的第一亮度数据和移位后检测到的第二亮度数据来计算多个光电探测器13 i的灵敏度波动。 然后，校正第一亮度数据或第二亮度数据。

公开(公告)号：US07312920B2

公开(公告)日：2007-12-25

申请号：US11232033

申请日：2005-09-22

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/00

CPC分类号： G02B21/0048 ,  G02B26/12

摘要： A confocal microscope including a first condenser optical system that condenses light from a light source onto a sample; a pinhole member having a pinhole; a second condenser optical system that condenses light from the sample into the pinhole; a polygon mirror having a plurality of mirror surfaces achieving angles relative to a rotational axis different from one another and formed so as to surround the rotational axis in a full circle, which is disposed between the first condenser optical system and the pinhole; and a first optical system that separates the light from the light source and the light from the sample and guides the light from the sample departing the polygon mirror to the pinhole.

摘要翻译： 一种共聚焦显微镜，包括将来自光源的光聚集到样品上的第一聚光器光学系统; 具有针孔的针孔构件; 第二聚光器光学系统，其将来自样品的光冷凝到针孔中; 多面镜，其具有多个镜面，其相对于彼此不同的旋转轴线成角度并且形成为围绕设置在第一聚光镜系统和针孔之间的全圆的旋转轴线; 以及第一光学系统，其将来自光源的光和来自样品的光分离，并将来自离开多面镜的样品的光引导到针孔。

公开(公告)号：US20060066944A1

公开(公告)日：2006-03-30

申请号：US11232033

申请日：2005-09-22

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/00

CPC分类号： G02B21/0048 ,  G02B26/12

摘要： A confocal microscope includes: a first condenser optical system that condenses light from a light source onto a sample; a pinhole member having a pinhole; a second condenser optical system that condenses light from the sample into the pinhole; a polygon mirror having a plurality of mirror surfaces achieving angles relative to a rotational axis different from one another and formed so as to surround the rotational axis in a full circle, which is disposed between the first condenser optical system and the pinhole; and a first optical system that separates the light from the light source and the light from the sample and guides the light from the sample departing the polygon mirror to the pinhole.

摘要翻译： 共焦显微镜包括：将来自光源的光冷凝到样品上的第一聚光器光学系统; 具有针孔的针孔构件; 第二聚光器光学系统，其将来自样品的光冷凝到针孔中; 多面镜，其具有多个镜面，其相对于彼此不同的旋转轴线成角度并且形成为围绕设置在第一聚光镜系统和针孔之间的全圆的旋转轴线; 以及第一光学系统，其将来自光源的光和来自样品的光分离，并将来自离开多面镜的样品的光引导到针孔。

公开(公告)号：US5477013A

公开(公告)日：1995-12-19

申请号：US181459

申请日：1994-01-14

申请人： Hisashi Okugawa ,  Akira Adachi

发明人： Hisashi Okugawa ,  Akira Adachi

IPC分类号： B41J2/44 ,  G02B26/10 ,  G06K7/10 ,  G10K11/16 ,  G10K11/04

CPC分类号： G10K11/16

摘要： A soundproofing device for a resonant scanner for deflecting a laser beam includes a plate member for fixing the resonant scanner, a plurality of stanchions for supporting the plate member, and a base for fixing the stanchions. The stanchions support the plate member at a position at which an amplitude of the plate member at a resonant frequency of the resonant scanner is minimum.

摘要翻译： 用于偏转激光束的谐振扫描器的隔音装置包括用于固定共振扫描器的板构件，用于支撑板构件的多个支柱和用于固定支柱的基座。 所述支柱将所述板构件支撑在所述共振扫描器的谐振频率处的所述板构件的振幅最小的位置处。