公开(公告)号：US08310754B2

公开(公告)日：2012-11-13

申请号：US12748031

申请日：2010-03-26

申请人： Hisashi Okugawa ,  Naoshi Aikawa ,  Masatoshi Sato

发明人： Hisashi Okugawa ,  Naoshi Aikawa ,  Masatoshi Sato

IPC分类号： G02B21/06 ,  G01J3/30

CPC分类号： G02B21/0076 ,  G02B5/0833 ,  G02B5/285

摘要： The present application has a proposition to provide a highly efficient laser excitation fluorescent microscope. Accordingly, a laser excitation fluorescent microscope of the present application includes a laser light source part radiating at least two types of excitation lights having different wavelengths; a light collecting part collecting the two types of excitation lights on a sample; a high-functional dichroic mirror, disposed between the laser light source part and the light collecting part, reflecting the two types of excitation lights to make the excitation lights incident on the light collecting part, and transmitting two types of fluorescence generated at the sample; and a detecting part detecting light transmitted through the high-functional dichroic mirror, in which an incident angle θ of the excitation lights and the fluorescence to the high-functional dichroic mirror satisfies a formula of 0°

摘要翻译： 本申请提出了一种提供高效激光荧光显微镜的命题。 因此，本申请的激光激发荧光显微镜包括：照射具有不同波长的至少两种激发光的激光光源部; 收集样品上的两种激发光的集光部分; 设置在激光光源部和集光部之间的高分辨率反射镜，反射这两种激发光，使激发光入射到聚光部上，并透射在样品上产生的两种荧光; 以及检测部，其检测透过所述高功能分色镜的光，其中入射角＆ 的激发光和对高功能二向色镜的荧光满足0°<＆amp; t; <45°的公式。

公开(公告)号：US07724365B2

公开(公告)日：2010-05-25

申请号：US12292924

申请日：2008-12-01

申请人： Yoko Fukuda ,  Hisashi Okugawa

发明人： Yoko Fukuda ,  Hisashi Okugawa

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6419 ,  G01N2021/6441 ,  G02B21/0032 ,  G02B21/0076

摘要： A proposition of the present invention is to acquire necessary data without any chasm in an observation of a specimen containing plural kinds of substances of which exciting wavelengths are different. Accordingly, a spectral observation method of the present invention, irradiating light to the specimen containing plural kinds of substances (RFP, GFP, and so on) of which exciting wavelengths are different, and detecting spectra of light emitted from the specimen, the spectral observation method sequentially acquires spectral data of substances from the one of which exciting wavelength is long while switching a wavelength of the irradiated light among respective exciting wavelengths of the plural kinds of substances, and excludes the exciting wavelength of the substance corresponding to the spectral data to be acquired from detection wavelengths of the spectra each when acquiring the spectral data of the spectral data of the respective substances.

摘要翻译： 本发明的目的在于在观察包含多种激发波长不同的物质的样本时获得必要的数据，而没有任何裂缝。 因此，本发明的光谱观察方法，对含有激发波长不同的多种物质（RFP，GFP等）的样本照射光并检测从样品发出的光的光谱，光谱观察 方法顺序地从激光波长较长的物质中获取光谱数据，同时在多种物质的各个激发波长之间切换照射光的波长，并且将与光谱数据对应的物质的激发波长排除为 在获取各物质的光谱数据的光谱数据时从光谱的检测波长中获取每个光谱。

公开(公告)号：US20090116008A1

公开(公告)日：2009-05-07

申请号：US12292924

申请日：2008-12-01

申请人： Yoko Fukuda ,  Hisashi Okugawa

发明人： Yoko Fukuda ,  Hisashi Okugawa

IPC分类号： G01N21/25

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6419 ,  G01N2021/6441 ,  G02B21/0032 ,  G02B21/0076

摘要： A proposition of the present invention is to acquire necessary data without any chasm in an observation of a specimen containing plural kinds of substances of which exciting wavelengths are different. Accordingly, a spectral observation method of the present invention, irradiating light to the specimen containing plural kinds of substances (RFP, GFP, and so on) of which exciting wavelengths are different, and detecting spectra of light emitted from the specimen, the spectral observation method sequentially acquires spectral data of substances from the one of which exciting wavelength is long while switching a wavelength of the irradiated light among respective exciting wavelengths of the plural kinds of substances, and excludes the exciting wavelength of the substance corresponding to the spectral data to be acquired from detection wavelengths of the spectra each when acquiring the spectral data of the spectral data of the respective substances.

摘要翻译： 本发明的目的在于在观察包含多种激发波长不同的物质的样本时获得必要的数据，而没有任何裂缝。 因此，本发明的光谱观察方法，对含有激发波长不同的多种物质（RFP，GFP等）的样本照射光并检测从样品发出的光的光谱，光谱观察 方法顺序地从激光波长较长的物质中获取光谱数据，同时在多种物质的各个激发波长之间切换照射光的波长，并且将与光谱数据对应的物质的激发波长排除为 在获取各物质的光谱数据的光谱数据时从光谱的检测波长中获取每个光谱。

公开(公告)号：US20090010504A1

公开(公告)日：2009-01-08

申请号：US11817405

申请日：2006-06-20

申请人： Hisashi Okugawa ,  Kensaku Fukumoto

发明人： Hisashi Okugawa ,  Kensaku Fukumoto

IPC分类号： G06K9/00

CPC分类号： G02B21/008

摘要： A confocal microscope apparatus capable of increasing a degree of freedom in varying sectioning resolution while keeping a configuration of a confocal microscope simple. The confocal microscope apparatus includes a confocal microscope capable of detecting two or more microscopic appearances with different sectioning and an arithmetic unit that performs arithmetic operations on data of the two or more microscopic appearances detected by the confocal microscope to create data of a microscopic appearance at sectioning resolution different from that of those microscopic appearances. Even if the number of actually measured microscopic appearances is two, it is possible to vary the diameter of a virtual-confocal diaphragm by performing arithmetic operations thereon, and therefore, an arbitrary sectioning resolution can be realized.

摘要翻译： 能够在保持共焦显微镜的构造的同时，能够提高变化的分割分辨率的自由度的共聚焦显微镜装置。 共焦显微镜装置包括能够检测两个或多个具有不同切片的微观外观的共焦显微镜，以及对由共焦显微镜检测的两个或多个微观外观的数据进行算术运算的算术单元，以在切片处产生微观外观的数据 分辨率与那些微观外观不同。 即使实际测量的微观外观的数量为2，可以通过对其进行算术运算来改变虚拟共焦膜的直径，因此可以实现任意的切片分辨率。

公开(公告)号：US20080291534A1

公开(公告)日：2008-11-27

申请号：US12010712

申请日：2008-01-29

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/30 ,  G02B21/06

CPC分类号： G02B21/0088 ,  G02B21/26 ,  G02B21/30 ,  G02B21/32

摘要： A microscope system includes a casing in which an optical element is housed, an airtight specimen chamber, and an environment control unit that controls temperature and humidity in the specimen chamber and also controls temperature inside the casing. An upper plate of the specimen chamber is an open/close lid opened and closed when inserting or removing a biological specimen. The casing becomes sealed by a bottom plate of the specimen chamber as the specimen chamber is set on the casing.

公开(公告)号：US20060072190A1

公开(公告)日：2006-04-06

申请号：US11286293

申请日：2005-11-25

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/00

CPC分类号： G02B21/0088 ,  G02B21/26 ,  G02B21/30 ,  G02B21/32

摘要： A microscope system includes a casing in which an optical element is housed, an airtight specimen chamber, and an environment control unit that controls temperature and humidity in the specimen chamber and also controls temperature inside the casing. An upper plate of the specimen chamber is an open/close lid opened and closed when inserting or removing a biological specimen. The casing becomes sealed by a bottom plate of the specimen chamber as the specimen chamber is set on the casing.

公开(公告)号：US06906859B2

公开(公告)日：2005-06-14

申请号：US10453660

申请日：2003-06-04

申请人： Toshiaki Nihoshi ,  Hisashi Okugawa

发明人： Toshiaki Nihoshi ,  Hisashi Okugawa

IPC分类号： G02B21/06 ,  G02B21/08 ,  G02B21/16

CPC分类号： G02B21/08 ,  G02B21/06 ,  G02B21/16

摘要： An epi-illumination apparatus for fluorescent observation, adjusting light intensities of a plurality of illuminations on a sample over a wide wavelength band continuously and being configured inexpensively, and a fluorescence microscope having the same are provided. A light source, extracting means, an aperture stop, and a filter are arranged on a predetermined optical axis. The aperture stop is arranged on a plane generally conjugated with a pupil plane of an objective. The filter is placed near the aperture stop. The extracting means extracts a plurality of narrow wavelength bands from the wavelength band of the illumination emitted from the light source. The filter has regions of different spectral transmission characteristics to the narrow wavelength bands. The adjusting means for adjusting light intensities of transmitted light from the filter in the narrow wavelength bands independently by moving the filter in a direction orthogonal to the optical axis is provided.

摘要翻译： 一种用于荧光观察的外照明装置，连续地调节宽波长带上的样品上的多个照明的光强度并且被廉价地配置，并且提供具有该照明装置的荧光显微镜。 在预定的光轴上布置有光源，提取装置，孔径光阑和滤光器。 孔径光阑布置在通常与物镜的光瞳平面共轭的平面上。 过滤器放置在孔径光阑附近。 提取装置从从光源发射的照明的波长带中提取多个窄波长带。 滤波器具有与窄波长带不同的频谱传输特性的区域。 提供了一种调节装置，用于通过沿与光轴正交的方向移动滤光器来独立地调节来自窄波长带中的滤光器的透射光的光强度。

公开(公告)号：US20100020392A1

公开(公告)日：2010-01-28

申请号：US12585890

申请日：2009-09-28

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/06

CPC分类号： G02B21/008

摘要： The present application detects a state change of an object in an optical axis direction by a single frame scanning for each round. A confocal microscope apparatus includes a detecting unit separating incident light into a light from a vicinity of a collecting point on the sample and a light from a peripheral of the vicinity and detecting each of the lights, and an image generating unit generating an image of the sample by a light signal from the vicinity and a light signal from the peripheral, in which a distinction between a light signal from the vicinity and a light signal from the peripheral is calculated for each of a plurality of images generated in a number of times of scanning without changing a collecting location in an optical axis direction.

摘要翻译： 本申请通过每轮的单帧扫描来检测物体在光轴方向上的状态变化。 共聚焦显微镜装置包括检测单元，其将入射光分离成来自样品上的收集点附近的光和来自附近的周边的光并检测每个光;以及图像生成单元，生成图像的图像 通过来自附近的光信号进行采样以及来自外围设备的光信号，其中计算来自附近的光信号与来自外围设备的光信号之间的区别，其中对于多次产生的多个图像中的每一个， 扫描而不改变光轴方向的收集位置。

公开(公告)号：US20090080722A1

公开(公告)日：2009-03-26

申请号：US11913281

申请日：2007-02-01

申请人： Hisashi Okugawa ,  Masafumi Mimura

发明人： Hisashi Okugawa ,  Masafumi Mimura

IPC分类号： G06K9/00

CPC分类号： G01N21/274 ,  G01N21/6458 ,  G06T5/002 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30024 ,  H04N1/409

摘要： A spectral image processing method is capable of reducing noise while maintaining necessary information. The spectral image processing method performs processing on a spectral image of a specimen, including a step of normalizing spectra (=spectral brightness curves) of respective pixels constituting the spectral image such that their brightness levels become equal, a step of smoothing the normalized spectra in spatial directions of the respective pixels, and a step of denormalization of multiplying spectra of the respective pixels obtained by the smoothing by either one of brightness levels of the pixels corresponding the spectra and values corresponding to the brightness levels. Consequently, the noise can be reduced while information on brightness distribution on the image is maintained.

摘要翻译： 光谱图像处理方法能够在保持必要信息的同时降低噪声。 光谱图像处理方法对样本的光谱图像执行处理，包括使构成光谱图像的各个像素的光谱（=光谱亮​​度曲线）归一化为使得它们的亮度水平变得相等的步骤，使平均化的归一化光谱的步骤 各个像素的空间方向，以及通过平滑获得的各个像素的乘积光谱的非归一化的步骤，对应于对应于亮度等级的光谱和值的像素的亮度级中的任一个。 因此，可以降低噪声，同时保持图像上亮度分布的信息。

公开(公告)号：US07330257B2

公开(公告)日：2008-02-12

申请号：US11207872

申请日：2005-08-22

申请人： Yoshinori Kuroiwa ,  Hisashi Okugawa

发明人： Yoshinori Kuroiwa ,  Hisashi Okugawa

IPC分类号： G01J3/28

CPC分类号： G01N21/6458 ,  G01J3/02 ,  G01J3/0237 ,  G01J3/28 ,  G01J3/2803 ,  G01J3/4406 ,  G01N2021/6421

摘要： An object is to provide a spectroscope and a spectrum laser microscope capable of carrying out sensitivity correction of a multi-channel photodetector with real time. The spectrum laser microscope 100 includes a laser microscope 101 and a spectral analyzer 103 having a multi-channel photodetector 13 composed of a plurality of photodetectors 13i for detecting spectral distribution of the light from the laser microscope 101. Sensitivity fluctuation of the plurality of photodetectors 13i is calculated from a first luminance data detected before shifting relative position between the spectra and the multi-channel photodetector 13 and a second luminance data detected after shifting. Then, the first luminance data or the second luminance data is corrected.

摘要翻译： 目的是提供能够实时地进行多通道光电检测器的灵敏度校正的分光计和光谱激光显微镜。 光谱激光显微镜100包括激光显微镜101和具有由用于检测来自激光显微镜101的光的光谱分布的多个光电检测器13i组成的多通道光电检测器13的光谱分析器103。 根据在频谱和多声道光电探测器13之间的相对位置移动之前检测到的第一亮度数据和移位后检测到的第二亮度数据来计算多个光电探测器13 i的灵敏度波动。 然后，校正第一亮度数据或第二亮度数据。