公开(公告)号：US20090080722A1

公开(公告)日：2009-03-26

申请号：US11913281

申请日：2007-02-01

申请人： Hisashi Okugawa ,  Masafumi Mimura

发明人： Hisashi Okugawa ,  Masafumi Mimura

IPC分类号： G06K9/00

CPC分类号： G01N21/274 ,  G01N21/6458 ,  G06T5/002 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30024 ,  H04N1/409

摘要： A spectral image processing method is capable of reducing noise while maintaining necessary information. The spectral image processing method performs processing on a spectral image of a specimen, including a step of normalizing spectra (=spectral brightness curves) of respective pixels constituting the spectral image such that their brightness levels become equal, a step of smoothing the normalized spectra in spatial directions of the respective pixels, and a step of denormalization of multiplying spectra of the respective pixels obtained by the smoothing by either one of brightness levels of the pixels corresponding the spectra and values corresponding to the brightness levels. Consequently, the noise can be reduced while information on brightness distribution on the image is maintained.

摘要翻译： 光谱图像处理方法能够在保持必要信息的同时降低噪声。 光谱图像处理方法对样本的光谱图像执行处理，包括使构成光谱图像的各个像素的光谱（=光谱亮​​度曲线）归一化为使得它们的亮度水平变得相等的步骤，使平均化的归一化光谱的步骤 各个像素的空间方向，以及通过平滑获得的各个像素的乘积光谱的非归一化的步骤，对应于对应于亮度等级的光谱和值的像素的亮度级中的任一个。 因此，可以降低噪声，同时保持图像上亮度分布的信息。

公开(公告)号：US08055035B2

公开(公告)日：2011-11-08

申请号：US11913281

申请日：2007-02-01

申请人： Hisashi Okugawa ,  Masafumi Mimura

发明人： Hisashi Okugawa ,  Masafumi Mimura

IPC分类号： G06K9/00

CPC分类号： G01N21/274 ,  G01N21/6458 ,  G06T5/002 ,  G06T5/50 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30024 ,  H04N1/409

摘要： A spectral image processing method is capable of reducing noise while maintaining necessary information. The spectral image processing method performs processing on a spectral image of a specimen, including a step of normalizing spectra (=spectral brightness curves) of respective pixels constituting the spectral image such that their brightness levels become equal, a step of smoothing the normalized spectra in spatial directions of the respective pixels, and a step of denormalization of multiplying spectra of the respective pixels obtained by the smoothing by either one of brightness levels of the pixels corresponding the spectra and values corresponding to the brightness levels. Consequently, the noise can be reduced while information on brightness distribution on the image is maintained.

摘要翻译： 光谱图像处理方法能够在保持必要信息的同时降低噪声。 光谱图像处理方法对样本的光谱图像执行处理，包括使构成光谱图像的各个像素的光谱（=光谱亮​​度曲线）归一化为使得它们的亮度水平变得相等的步骤，使平均化的归一化光谱的步骤 各个像素的空间方向，以及通过平滑获得的各个像素的乘积光谱的非归一化的步骤，对应于对应于亮度等级的光谱和值的像素的亮度级中的任一个。 因此，可以降低噪声，同时保持图像上亮度分布的信息。

公开(公告)号：US08045153B2

公开(公告)日：2011-10-25

申请号：US11913294

申请日：2007-02-01

申请人： Masafumi Mimura ,  Hisashi Okugawa

发明人： Masafumi Mimura ,  Hisashi Okugawa

IPC分类号： G01J3/30 ,  G06K9/00 ,  G01N33/48

CPC分类号： G01N21/6458 ,  G01N2021/6417 ,  G01N2021/6441

摘要： A spectral image processing system and method of performing robust unmixing on measurement noise. Based on an observed spectral image acquired from a specimen and emission spectral data of each of plural materials contained in the specimen, a contribution of each of the plural materials to the observed spectral image is unmixed by a process, including an evaluating step of evaluating reliability of each component of the observed spectral image based on a predicted spectral image of the observed spectral image, and a reflecting step of reflecting a result of the evaluation in a content of the unmixing.

摘要翻译： 一种对测量噪声进行稳健解混的光谱图像处理系统和方法。 基于从样本获取的观察到的光谱图像和包含在样本中的多种材料的发射光谱数据，通过包括评价可靠性的评估步骤的处理，将多种材料中的每一种对所观察的光谱图像的贡献未混合 基于观测到的光谱图像的预测光谱图像，以及将评价结果反映在解混合物的内容中的反射步骤。

公开(公告)号：US20090128806A1

公开(公告)日：2009-05-21

申请号：US11913294

申请日：2007-02-01

申请人： Masafumi Mimura ,  Hisashi Okugawa

发明人： Masafumi Mimura ,  Hisashi Okugawa

IPC分类号： G01J3/30

CPC分类号： G01N21/6458 ,  G01N2021/6417 ,  G01N2021/6441

摘要： A spectral image processing system and method of performing robust unmixing on measurement noise. Based on an observed spectral image acquired from a specimen and emission spectral data of each of plural materials contained in the specimen, a contribution of each of the plural materials to the observed spectral image is unmixed by a process, including an evaluating step of evaluating reliability of each component of the observed spectral image based on a predicted spectral image of the observed spectral image, and a reflecting step of reflecting a result of the evaluation in a content of the unmixing.

摘要翻译： 一种对测量噪声进行稳健解混的光谱图像处理系统和方法。 基于从样本获取的观察到的光谱图像和包含在样本中的多种材料的发射光谱数据，通过包括评价可靠性的评估步骤的处理，将多种材料中的每一种对所观察的光谱图像的贡献未混合 基于观测到的光谱图像的预测光谱图像，以及将评价结果反映在解混合物的内容中的反射步骤。

公开(公告)号：US08902306B2

公开(公告)日：2014-12-02

申请号：US12929369

申请日：2011-01-19

申请人： Masafumi Mimura ,  Kei Ito

发明人： Masafumi Mimura ,  Kei Ito

IPC分类号： H04N7/18 ,  G06K9/00 ,  G02B21/00 ,  G02B21/36 ,  G06T7/40 ,  C12M1/34 ,  C12M3/00 ,  C12M1/00 ,  G06T7/00 ,  G06T7/20 ,  G06K9/62

CPC分类号： G02B21/365 ,  C12M23/48 ,  C12M41/14 ,  C12M41/36 ,  C12M41/46 ,  G02B21/0088 ,  G06K9/00147 ,  G06T7/0012 ,  G06T7/0016 ,  G06T7/20 ,  G06T7/33 ,  G06T7/41 ,  G06T2207/10056 ,  G06T2207/30024

摘要： An image processing device determines the degree of activity of a cell during cell viewing. An imaging device captures first and second images of a cell under observation. After aligning the rotational angle orientations on the image plane of the cell under observation that is included in the images, a correlation value or difference of intracellular texture feature values in the first image and second image, is sequentially computed. A time series variation of the computed correlation value or difference is then derived, and the degree of activity of the cell under observation is detected based on the state of variation.

摘要翻译： 图像处理装置确定细胞观察期间细胞的活性程度。 成像装置捕获观察下的细胞的第一和第二图像。 在对包含在图像中观察的单元的图像平面上的旋转角度取向进行对准之后，依次计算第一图像和第二图像中的细胞内结构特征值的相关值或差异。 然后导出所计算的相关值或差的时间序列变化，并且基于变化的状态来检测观察的细胞的活动程度。

公开(公告)号：US08478017B2

公开(公告)日：2013-07-02

申请号：US12923144

申请日：2010-09-03

申请人： Masafumi Mimura ,  Kei Ito

发明人： Masafumi Mimura ,  Kei Ito

IPC分类号： G06K9/00

CPC分类号： G06K9/0014 ,  C12M23/48 ,  C12M41/14 ,  C12M41/36 ,  C12M41/46 ,  G06T7/0012 ,  G06T7/254 ,  G06T2207/10056 ,  G06T2207/30024

摘要： An image processing program for cell observation in which living cells can be distinguished from foreign matter other than living cells during cell observation under wide observation conditions. The image processing program includes a step for capturing first and second images of an object positioned in the observation field of view at a predetermined time interval, a step for extracting a representation of the object shown in the obtained images, steps for aligning the rotational angular orientation of the object in the image plane and computing a correlation value, a step for determining whether the object is a living cell on the basis of the computed correlation value, and steps for outputting the result of the decision made with regard to the object.

摘要翻译： 一种用于细胞观察的图像处理程序，其中在广泛的观察条件下，细胞观察期间活细胞可以与活细胞以外的异物区分开。 图像处理程序包括以预定时间间隔捕获位于观察视野中的物体的第一和第二图像的步​​骤，用于提取所获得图像中所示的对象的表示的步骤，用于对准旋转角 并且计算相关值，基于计算的相关值来确定对象是活细胞的步骤，以及用于输出关于对象的决定结果的步骤。

公开(公告)号：US08503790B2

公开(公告)日：2013-08-06

申请号：US12801666

申请日：2010-06-18

申请人： Kei Ito ,  Masafumi Mimura

发明人： Kei Ito ,  Masafumi Mimura

IPC分类号： G06K9/46

CPC分类号： C12M41/14 ,  C12M41/46 ,  G06T7/32 ,  G06T7/33 ,  G06T2207/10016 ,  G06T2207/30024 ,  H04N9/8233

摘要： An image-processing method for processing a time lapse image includes acquiring a first image obtained by capturing an image of an object located within a field of view using an imaging device and a second image obtained by capturing an image of the object located within the field of view using the imaging device after a predetermined time period has elapsed, calculating a positional correction value for a positional shift between the first image and the second image, by using a correlation function weighted according to an image feature of each of the objects included in the first image and the second image, and performing, based on the calculated positional correction value, a positional correction between the first image and the second image. The time lapse image is generated using the first image and the second image on which the positional correction has been performed.

摘要翻译： 用于处理时间流逝图像的图像处理方法包括获取通过使用成像装置捕获位于视场内的对象的图像获得的第一图像和通过捕获位于场内的对象的图像而获得的第二图像 在经过预定时间段之后使用成像装置的视角，通过使用根据包括在每个对象中的每个对象的图像特征加权的相关函数来计算第一图像和第二图像之间的位置偏移的位置校正值 第一图像和第二图像，并且基于计算的位置校正值来执行第一图像和第二图像之间的位置校正。 使用执行了位置校正的第一图像和第二图像来生成时间流逝图像。

公开(公告)号：US20110228069A1

公开(公告)日：2011-09-22

申请号：US12929369

申请日：2011-01-19

申请人： Masafumi Mimura ,  Kei Ito

发明人： Masafumi Mimura ,  Kei Ito

IPC分类号： G06K9/00 ,  H04N7/18

CPC分类号： G02B21/365 ,  C12M23/48 ,  C12M41/14 ,  C12M41/36 ,  C12M41/46 ,  G02B21/0088 ,  G06K9/00147 ,  G06T7/0012 ,  G06T7/0016 ,  G06T7/20 ,  G06T7/33 ,  G06T7/41 ,  G06T2207/10056 ,  G06T2207/30024

摘要： Provided is a means whereby the degree of activity of a cell can be determined during cell viewing. By an imaging device, first and second images in which a cell under observation is captured are sequentially obtained at a predetermined time interval, and after aligning the rotational angle orientations on the image plane of the cell under observation that is included in the images, a correlation value or difference of intracellular texture feature values in the first image and second image is sequentially computed. A time series variation of the computed correlation value or difference is then derived, and the degree of activity of the cell under observation is detected based on the state of variation.

摘要翻译： 提供了一种可以在细胞观察期间确定细胞的活性程度的方法。 通过成像装置，以预定的时间间隔顺序地获得其中捕获观察的细胞的第一和第二图像，并且在包括在图像中的观察下的细胞的像面上对准旋转角度取向之后， 依次计算第一图像和第二图像中的相关值或细胞内结构特征值的差异。 然后导出所计算的相关值或差的时间序列变化，并且基于变化的状态来检测观察的细胞的活动程度。

公开(公告)号：US20110019923A1

公开(公告)日：2011-01-27

申请号：US12923513

申请日：2010-09-24

申请人： Masafumi Mimura ,  Kei Ito

发明人： Masafumi Mimura ,  Kei Ito

IPC分类号： G06K9/46

CPC分类号： G06K9/00134 ,  C12M41/14 ,  C12M41/46 ,  C12M41/48 ,  G06T7/12 ,  G06T7/251 ,  G06T2207/10056 ,  G06T2207/30024

摘要： The contour of a cell being observed is extracted from a cell observation image obtained by an imaging device, and a cell model made by modeling the outer shape of the cell is adapted. The direction in which the cell being observed is predicted to move is derived using the adapted cell model.

摘要翻译： 观察到的细胞的轮廓是从通过成像装置得到的细胞观察图像中提取出来的，通过对细胞的外形进行建模而形成的细胞模型是适合的。 使用适应的细胞模型导出观察细胞被预测移动的方向。

公开(公告)号：US20100260422A1

公开(公告)日：2010-10-14

申请号：US12801666

申请日：2010-06-18

申请人： Kei Ito ,  Masafumi Mimura

发明人： Kei Ito ,  Masafumi Mimura

IPC分类号： G06K9/46 ,  G06K9/64

CPC分类号： C12M41/14 ,  C12M41/46 ,  G06T7/32 ,  G06T7/33 ,  G06T2207/10016 ,  G06T2207/30024 ,  H04N9/8233

摘要： An image-processing method for processing a time lapse image includes acquiring a first image obtained by capturing an image of an object located within a field of view using an imaging device and a second image obtained by capturing an image of the object located within the field of view using the imaging device after a predetermined time period has elapsed, calculating a positional correction value for a positional shift between the first image and the second image, by using a correlation function weighted according to an image feature of each of the objects included in the fist image and the second image, and performing, based on the calculated positional correction value, a positional correction between the first image and the second image. The time lapse image is generated using the first image and the second image on which the positional correction has been performed.

摘要翻译： 用于处理时间流逝图像的图像处理方法包括获取通过使用成像装置捕获位于视场内的对象的图像获得的第一图像和通过捕获位于场内的对象的图像而获得的第二图像 在经过预定时间段之后使用成像装置的视角，通过使用根据包括在每个对象中的每个对象的图像特征加权的相关函数来计算第一图像和第二图像之间的位置偏移的位置校正值 所述第一图像和所述第二图像，并且基于所计算的位置校正值，执行所述第一图像和所述第二图像之间的位置校正。 使用执行了位置校正的第一图像和第二图像来生成时间流逝图像。