摘要:
It is an object of the present invention to clarify an enzyme gene capable of directly regulating the amount of active cytokinins synthesized and to provide a transformed plant with a regulated amount of cytokinins using the aforementioned gene.The present invention provides a transformed plant into which a gene defined in any one of the following (a) to (d) is introduced: (a) a gene consisting of a DNA consisting of the nucleotide sequence as shown in any one of SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13 and 15; (b) a gene consisting of a DNA which hybridizes under stringent conditions to a DNA consisting of a nucleotide sequence complementary to a DNA consisting of the nucleotide sequence as shown in any one of SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13 and 15 and encodes a protein having activity of catalyzing reactions that synthesize active cytokinins from nucleotide cytokinins; (c) a gene encoding a protein consisting of the amino acid sequence as shown in any one of SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14 and 16; or (d) a gene encoding a protein which consists of an amino acid sequence derived from the amino acid sequence as shown in any one of SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14 and 16 by deletion, substitution or addition of one or several amino acid residues and has activity of catalyzing reactions that synthesize active cytokinins from nucleotide cytokinins.
摘要翻译:本发明的目的是澄清能够直接调节合成的活性细胞分裂素的量并使用上述基因提供具有调节量的细胞分裂素的转化植物的酶基因。 本发明提供一种转化植物,其中引入以下(a)至(d)中任一项所定义的基因:(a)由SEQ ID NO:1中任一项所示的核苷酸序列组成的DNA组成的基因 NOS:1,3,5,7,9,11,13和15; (b)由在严格条件下与由与SEQ ID NO:1,3,5,7,9中任一项所示的核苷酸序列组成的DNA互补的核苷酸序列组成的DNA组成的DNA组成的基因 ,11,13和15,并编码具有催化从核苷酸细胞分裂素合成活性细胞分裂素的反应活性的蛋白质; (c)编码由SEQ ID NO:2,4,6,8,10,12,14和16中任一项所示的氨基酸序列组成的蛋白质的基因; 或(d)编码由SEQ ID NO:2,4,6,8,10,12,14和16中任一项所示的氨基酸序列的氨基酸序列组成的蛋白质的基因,通过缺失, 取代或添加一个或几个氨基酸残基,并且具有催化从核苷酸细胞分裂素合成活性细胞分裂素的反应的活性。
摘要:
Cytokinin is a plant hormone that regulates various physiological activities including cell division, cell cycle, cellular senescence, and axillary buds outgrowth. The present invention provides a method for producing a transformed plant by introducing an isolated nucleic acid or recombinant vector containing the isolated nucleic acid into a plant cell such that the amount of active cytokinin synthesized from nucleotide cytokinin is increased in the plant cell and by regenerating a plant body from the plant cell.
摘要:
It is an object of the present invention to clarify an enzyme gene capable of directly regulating the amount of active cytokinins synthesized and to provide a transformed plant with a regulated amount of cytokinins using the aforementioned gene.The present invention provides a transformed plant into which a gene defined in any one of the following (a) to (d) is introduced: (a) a gene consisting of a DNA consisting of the nucleotide sequence as shown in any one of SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13 and 15; (b) a gene consisting of a DNA which hybridizes under stringent conditions to a DNA consisting of a nucleotide sequence complementary to a DNA consisting of the nucleotide sequence as shown in any one of SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13 and 15 and encodes a protein having activity of catalyzing reactions that synthesize active cytokinins from nucleotide cytokinins; (c) a gene encoding a protein consisting of the amino acid sequence as shown in any one of SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14 and 16; or (d) a gene encoding a protein which consists of an amino acid sequence derived from the amino acid sequence as shown in any one of SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14 and 16 by deletion, substitution or addition of one or several amino acid residues and has activity of catalyzing reactions that synthesize active cytokinins from nucleotide cytokinins.
摘要翻译:本发明的目的是澄清能够直接调节合成的活性细胞分裂素的量并使用上述基因提供具有调节量的细胞分裂素的转化植物的酶基因。 本发明提供一种转化植物,其中引入以下(a)至(d)中任一项所定义的基因:(a)由SEQ ID NO:1中任一项所示的核苷酸序列组成的DNA组成的基因 NOS:1,3,5,7,9,11,13和15; (b)由在严格条件下与由与SEQ ID NO:1,3,5,7,9中任一项所示的核苷酸序列组成的DNA互补的核苷酸序列组成的DNA组成的DNA组成的基因 ,11,13和15,并编码具有催化从核苷酸细胞分裂素合成活性细胞分裂素的反应活性的蛋白质; (c)编码由SEQ ID NO:2,4,6,8,10,12,14和16中任一项所示的氨基酸序列组成的蛋白质的基因; 或(d)编码由SEQ ID NO:2,4,6,8,10,12,14和16中任一项所示的氨基酸序列的氨基酸序列组成的蛋白质的基因,通过缺失, 取代或添加一个或几个氨基酸残基,并且具有催化从核苷酸细胞分裂素合成活性细胞分裂素的反应的活性。
摘要:
An object of the present invention is to provide a means for conferring a useful phenotype to a plant by efficiently controlling the quantitative productivity of iP-type and tZ-type active cytokinins in plant aerial organs. According to the present invention, a method for increasing plant biomass by causing the overexpression of an active cytokinin synthase gene and a cytokinin hydroxylase gene in a plant body is provided.
摘要:
Disclosed is a method for recovery of a metal using plants. The method for recovery of a metal involves bringing a protonema of a moss plant belonging to the family Funariaceae into contact with a metal-containing solution in which a metal(s) having an ionization tendency lower than that of silver is dissolved.
摘要:
A method of producing an extract derived from Lyophyllum ulmarium from fruiting body or mycelium of Lyophyllum ulmarium, wherein the method of producing an extract derived from Lyophyllum ulmarium uses an aqueous alkaline solvent as an extraction solvent; an extract derived from Lyophyllum ulmarium, obtainable by the method as defined above; and a food and a medicament each containing the extract.
摘要:
An object of the present invention is to provide a transgenic plant on which a useful phenotype is conferred by regulating expression of pseudo-response regulator (PRR) genes with an approach that is different from deletion of PRR genes. According to the present invention, there is provided a transgenic plant into which a polynucleotide encoding a protein comprising a CCT motif and a pseudo-receiver domain which is a common motif of plant pseudo-response regulator genes, a fusion polynucleotide of said polynucleotide and a polynucleotide encoding at least one transcription activation domain of a transcription factor, or a recombinant vector comprising said polynucleotide or fusion polynucleotide has been introduced.
摘要:
Disclosed is a method for recovery of a metal using plants. The method for recovery of a metal involves bringing a protonema of a moss plant belonging to the family Funariaceae into contact with a metal-containing solution in which a metal(s) having an ionization tendency lower than that of silver is dissolved.
摘要:
According to the present invention, there are provided kneading-free Angelica keiskei tea leaves obtainable by a treatment comprising a step for roast-heating a dried material of Angelica keiskei; an Angelica keiskei tea beverage produced by using the kneading-free Angelica keiskei tea leaves; and a process for producing thereof. The Angelica keiskei tea leaves are preferably heated at a heating temperature of 100° C. to 150° C. in the roast-heating treatment, and the dried material of Angelica keiskei to be subjected to the roast-heating is preferably obtained by drying in a dried system after blanching and has a moisture content of 20% or less. Further, according to the present invention, there is also provided an Angelica keiskei chalcone function-formulated beverage comprising formulated Angelica keiskei chalcones that are components specific to Angelica keiskei.
摘要:
Operation time interval measurement means 28 of a passive exercise machine 1 measures the operation time interval and stores the measurement result of the operation time interval in association with the user identifiers in a machine operation table TB2. A server 2 includes an exercise history table TB12 that associates and stores the user identifiers and the measurement data on the operation time interval received by external communication means 41 from the passive exercise machine 1 via a network, operation time interval-to-exercise point conversion means 46 for determining exercise points that are obtained by quantifying the exercise amount of each user from the operation time interval of the user stored in the operation history table TB12 and storing the determined exercise points in the exercise history table TB12, and machine operation pattern determination means 47 for determining a machine operation pattern number indicating an operation state of the passive exercise machine 1 that is being used by the user when a cumulative total number of exercise points per user that has been stored in the exercise history table TB12 exceeds a predetermined threshold level. The server 2 transmits the determined machine operation pattern number from the external communication means 41 to the passive exercise machine 1.