公开(公告)号：US20140329252A1

公开(公告)日：2014-11-06

申请号：US14351398

申请日：2012-10-15

申请人： HOPITAL SAINTE-JUSTINE

发明人： Alain Moreau

IPC分类号： G01N33/573 ,  G01N33/569

CPC分类号： G01N33/6893 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/573 ,  G01N2333/47 ,  G01N2333/9015 ,  G01N2440/36 ,  G01N2800/105 ,  G01N2800/50

摘要： A method of determining whether a subject is at risk of developing osteoarthritis (OA), said method comprising: determining the cellular localization of a Prohibitin-1 (PHB1) polypeptide and/or Small Ubiquitin-like Modifier (SUMO) polypeptide and/or UBC9, in a cell sample from said subject; and determining whether said subject is at risk of developing OA based on the cellular localization of a PHB1 polypeptide and/or SUMO and/or UBC9 polypeptide, is described.

摘要翻译： 一种确定受试者是否处于发展为骨关节炎（OA）的风险的方法，所述方法包括：确定Prohibitin-1（PHB1）多肽和/或小泛素样修饰（SUMO）多肽和/或UBC9的细胞定位 在来自所述受试者的细胞样品中; 并且基于PHB1多肽和/或SUMO和/或UBC9多肽的细胞定位来确定所述受试者是否处于发展OA的风险。

公开(公告)号：US08652791B2

公开(公告)日：2014-02-18

申请号：US13726416

申请日：2012-12-24

申请人： Hopital Sainte-Justine

发明人： Alain Moreau

IPC分类号： G01N33/567 ,  C12Q1/02

CPC分类号： G01N33/566 ,  C12Q1/02 ,  C12Q1/527 ,  G01N33/56966 ,  G01N33/6893 ,  G01N33/74 ,  G01N2333/726 ,  G01N2800/10

摘要： A method of classifying a human subject having adolescent idiopathic scoliosis (AIS) comprising: providing a cell sample isolated from the subject; detecting an impairment in melatonin-signaling pathway in the sample in the presence and in the absence of a known melatonin-signaling pathway agonist, whereby the results of the detecting step enables the classification of the subject having AIS in one AIS subgroup; and a method of screening for a compound useful in the treatment of a disease characterized by a dysfunctional melatonin-signaling pathway, said method comprising the steps of contacting a candidate compound with at least one cell expressing at least one melatonin-signaling pathway impairment, wherein the candidate compound is selected if said melatonin-signaling pathway impairment is reduced in the presence of the candidate compound as compared to that in the absence thereof.

摘要翻译： 一种对具有青少年特发性脊柱侧凸（AIS）的人类受试者进行分类的方法，包括：提供从受试者分离的细胞样品; 在存在和不存在已知的褪黑激素信号通路激动剂的情况下检测样品中褪黑激素信号通路的损伤，由此检测步骤的结果使得能够在一个AIS亚组中对具有AIS的受试者进行分类; 以及筛选用于治疗以功能失调的褪黑激素信号传导途径为特征的疾病的化合物的方法，所述方法包括使候选化合物与至少一种表达至少一种褪黑激素信号传导途径损伤的细胞接触的步骤，其中 如果所述褪黑素信号传导途径损伤在候选化合物存在下降低而与不存在候选化合物相比，则选择候选化合物。