-
公开(公告)号:US07718362B2
公开(公告)日:2010-05-18
申请号:US10562803
申请日:2003-07-18
申请人: Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Yiming Zhou , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
发明人: Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Yiming Zhou , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
CPC分类号: C12Q1/6837
摘要: This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.
摘要翻译: 本发明一般涉及核酸分析领域。 特别地,本发明提供了一种用于对目标基因进行分型的方法,特别地,使用包含适合用于核酸杂交的载体的芯片,其上固定有与所述靶核苷酸序列互补的寡核苷酸探针和至少一种以下 寡核苷酸对照探针:阳性对照探针,阴性对照探针,杂交对照探针和固定化对照探针。 还提供了用于分型HLA靶基因的寡核苷酸探针或探针阵列。
-
公开(公告)号:US20070134661A1
公开(公告)日:2007-06-14
申请号:US10562803
申请日:2003-07-18
申请人: Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
发明人: Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
CPC分类号: C12Q1/6837
摘要: This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.
摘要翻译: 本发明一般涉及核酸分析领域。 特别地,本发明提供了一种用于对目标基因进行分型的方法,特别地,使用包含适合用于核酸杂交的载体的芯片,其上固定有与所述靶核苷酸序列互补的寡核苷酸探针和至少一种以下 寡核苷酸对照探针:阳性对照探针,阴性对照探针,杂交对照探针和固定化对照探针。 还提供了用于分型HLA靶基因的寡核苷酸探针或探针阵列。
-