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公开(公告)号：US20140024542A1
公开(公告)日：2014-01-23
申请号：US13551590
申请日：2012-07-17
CPC分类号： C12Q1/6806 , C12Q2525/155 , C12Q2535/122 , C12Q2563/131 , C12Q2563/149 , C12Q2563/179 , C12Q2565/543
摘要： The invention provides methods, apparatuses, and compositions for high-throughput amplification sequencing of specific target sequences in one or more samples. In some aspects, barcode-tagged polynucleotides are sequenced simultaneously and sample sources are identified on the basis of barcode sequences. In some aspects, sequencing data are used to determine one or more genotypes at one or more loci comprising a causal genetic variant.
摘要翻译： 本发明提供了用于在一个或多个样品中特异性靶序列的高通量扩增测序的方法，装置和组合物。 在一些方面，条形码标记的多核苷酸同时测序，并且基于条形码序列鉴定样品源。 在一些方面，使用测序数据来确定在一个或多个包含因果遗传变异体的基因座上的一种或多种基因型。
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公开(公告)号：US20140024541A1
公开(公告)日：2014-01-23
申请号：US13551587
申请日：2012-07-17
IPC分类号： C40B30/04
CPC分类号： C12Q1/6874 , C12Q2525/191 , C12Q2563/185 , C12Q2565/543
摘要： The invention provides methods, apparatuses, and compositions for high-throughput amplification sequencing of specific target sequences in one or more samples. In some aspects, barcode-tagged polynucleotides are sequenced simultaneously and sample sources are identified on the basis of barcode sequences. In some aspects, sequencing data are used to determine one or more genotypes at one or more loci comprising a causal genetic variant.
摘要翻译： 本发明提供了用于在一个或多个样品中特异性靶序列的高通量扩增测序的方法，装置和组合物。 在一些方面，条形码标记的多核苷酸同时测序，并且基于条形码序列鉴定样品源。 在一些方面，使用测序数据来确定在一个或多个包含因果遗传变异体的基因座上的一种或多种基因型。
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公开(公告)号：US20140024536A1
公开(公告)日：2014-01-23
申请号：US13551584
申请日：2012-07-17
CPC分类号： C12Q1/6874 , C40B50/18 , C12Q2525/161 , C12Q2535/122 , C12Q2537/159 , C12Q2565/543
摘要： The invention provides methods, apparatuses, and compositions for high-throughput amplification sequencing of specific target sequences in one or more samples. In some aspects, barcode-tagged polynucleotides are sequenced simultaneously and sample sources are identified on the basis of barcode sequences. In some aspects, sequencing data are used to determine one or more genotypes at one or more loci comprising a causal genetic variant.
摘要翻译： 本发明提供了用于在一个或多个样品中特异性靶序列的高通量扩增测序的方法，装置和组合物。 在一些方面，条形码标记的多核苷酸同时测序，并且基于条形码序列鉴定样品源。 在一些方面，使用测序数据来确定在一个或多个包含因果遗传变异体的基因座上的一种或多种基因型。
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公开(公告)号：US20230193247A1
公开(公告)日：2023-06-22
申请号：US17926566
申请日：2021-05-18
发明人： Clement Chu , Mark Theilmann , Noah Welker , Peter Grauman
IPC分类号： C12N15/10
CPC分类号： C12N15/1065
摘要： Described herein are methods for enriching test samples for target nucleic acid molecules for further genetic screening. Methods may comprise isolating nucleic acid from test subjects, preparing nucleic acid libraries wherein the nucleic acid molecules are tagged or barcoded to identify sample of origin, determining fragment size distribution, determining abundance of a target nucleic acid population, calculating numerical offset values to determine amount of libraries to add for fragment size selection, performing fragment size selection, and performing a diagnostic assay on a sample enriched for a target nucleic acid.
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