公开(公告)号：US20250163507A1

公开(公告)日：2025-05-22

申请号：US19030771

申请日：2025-01-17

Applicant: ILLUMINA, INC.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869

Abstract: A system for sequencing nucleic acid comprising a plurality of stations and a system control. The system control configured to direct the first substrate to progress and retrogress between the first processing station and the imaging station, direct the second substrate to progress and retrogress between the second processing station and the imaging station, and direct a chemistry cycle of a first sequencing procedure to occur within one of the first processing station or the second processing station while an imaging cycle of a second sequencing procedure occurs within the imaging station.

公开(公告)号：US09677194B2

公开(公告)日：2017-06-13

申请号：US13707337

申请日：2012-12-06

Applicant: ILLUMINA, INC.

Inventor： Michal Lebl ,  David L. Heiner ,  Chanfeng Zhao ,  David L. Barker

IPC: C40B50/06 ,  B01J19/00 ,  C40B40/06 ,  C40B50/14

CPC classification number: C40B50/06 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B50/14

Abstract: A method of fabricating a microarray including the steps of: (a) contacting a substrate having wells with a reagent reactive with said substrate to produce a surface modification within said wells and a surface modification surrounding said wells; (b) polishing said substrate to produce a polished surface modification surrounding said wells, wherein said surface modification surrounding said wells is removed and said surface modification within said wells is retained, and (c) depositing a biopolymer onto said substrate, wherein different affinities of said surface modification within said wells and said polished surface facilitate localization of said biopolymer within said wells.

公开(公告)号：US09121063B2

公开(公告)日：2015-09-01

申请号：US13653979

申请日：2012-10-17

Applicant: ILLUMINA, INC.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: G06F19/22 ,  C12Q1/68 ,  C12M1/34

CPC classification number: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2565/501 ,  C12Q2537/143

Abstract: A system for sequencing nucleic acids, that includes (a) a table having an arrangement of sites, including a site for receiving a first substrate, and a site for receiving a second substrate, the substrates each having an array for providing sequencing data for a plurality of different nucleic acids in parallel; (b) a plurality of stations configured to carry out manipulations in a sequencing procedure; and (c) a system control interface configured to direct relative movement between the table and the plurality of stations, and to direct different steps of the sequencing procedure to occur at the sites for receiving the different substrates, wherein the first substrate can be removed from the system independently of the second substrate such that the second substrate can be processed to obtain sequencing data independently of the first substrate.

Abstract translation: 一种用于对核酸进行测序的系统，其包括（a）具有位置排列的表，包括用于接收第一底物的位点和用于接收第二底物的位点，所述基底各自具有阵列，用于提供用于 多个不同的核酸并行; （b）多个站，被配置为在排序过程中执行操作; 以及（c）系统控制接口，被配置为引导所述台与所述多个站之间的相对运动，并且引导所述排序程序的不同步骤在用于接收所述不同衬底的位置处发生，其中所述第一衬底可以从 所述系统独立于所述第二衬底，使得可以处理所述第二衬底以获得独立于所述第一衬底的测序数据。

公开(公告)号：US20250011864A1

公开(公告)日：2025-01-09

申请号：US18887904

申请日：2024-09-17

Applicant: ILLUMINA, INC.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869

Abstract: A system for sequencing nucleic acid comprising a plurality of stations and a system control. The system control configured to direct the first substrate to progress and retrogress between the first processing station and the imaging station, direct the second substrate to progress and retrogress between the second processing station and the imaging station, and direct a chemistry cycle of a first sequencing procedure to occur within one of the first processing station or the second processing station while an imaging cycle of a second sequencing procedure occurs within the imaging station.

公开(公告)号：US20230076823A1

公开(公告)日：2023-03-09

申请号：US18050183

申请日：2022-10-27

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869

Abstract: A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

公开(公告)号：US20190085389A1

公开(公告)日：2019-03-21

申请号：US16105262

申请日：2018-08-20

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/686

Abstract: A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

公开(公告)号：US09797012B2

公开(公告)日：2017-10-24

申请号：US14539765

申请日：2014-11-12

Applicant: ILLUMINA, INC.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: C12Q1/68 ,  G06F19/22 ,  G06F19/20

CPC classification number: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/501 ,  C12Q2537/143

Abstract: A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

公开(公告)号：US11499191B2

公开(公告)日：2022-11-15

申请号：US16105262

申请日：2018-08-20

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869

Abstract: A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

公开(公告)号：US20130288931A1

公开(公告)日：2013-10-31

申请号：US13653979

申请日：2012-10-17

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  David L. Heiner ,  Chanfeng Zhao ,  Kevin Gunderson

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2565/501 ,  C12Q2537/143

Abstract: A system for sequencing nucleic acids, that includes (a) a table having an arrangement of sites, including a site for receiving a first substrate, and a site for receiving a second substrate, the substrates each having an array for providing sequencing data for a plurality of different nucleic acids in parallel; (b) a plurality of stations configured to carry out manipulations in a sequencing procedure; and (c) a system control interface configured to direct relative movement between the table and the plurality of stations, and to direct different steps of the sequencing procedure to occur at the sites for receiving the different substrates, wherein the first substrate can be removed from the system independently of the second substrate such that the second substrate can be processed to obtain sequencing data independently of the first substrate.

Abstract translation: 一种用于对核酸进行测序的系统，其包括（a）具有位置排列的表，包括用于接收第一底物的位点和用于接收第二底物的位点，所述基底各自具有阵列，用于提供用于 多个不同的核酸并行; （b）多个站，被配置为在排序过程中执行操作; 以及（c）系统控制接口，被配置为引导所述台与所述多个站之间的相对运动，并且引导所述排序程序的不同步骤在用于接收所述不同衬底的位置处发生，其中所述第一衬底可以从 所述系统独立于所述第二衬底，使得可以处理所述第二衬底以获得独立于所述第一衬底的测序数据。

公开(公告)号：US20130096034A1

公开(公告)日：2013-04-18

申请号：US13707337

申请日：2012-12-06

Applicant: ILLUMINA, INC.

Inventor： Michal Lebl ,  David L. Heiner ,  Chanfeng Zhao ,  David L. Barker

IPC: C40B50/06

CPC classification number: C40B50/06 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B50/14

Abstract: A method of fabricating a microarray including the steps of: (a) contacting a substrate having wells with a reagent reactive with said substrate to produce a surface modification within said wells and a surface modification surrounding said wells; (b) polishing said substrate to produce a polished surface modification surrounding said wells, wherein said surface modification surrounding said wells is removed and said surface modification within said wells is retained, and (c) depositing a biopolymer onto said substrate, wherein different affinities of said surface modification within said wells and said polished surface facilitate localization of said biopolymer within said wells.

Abstract translation: 一种制造微阵列的方法，包括以下步骤：（a）使具有孔的底物与具有与所述底物反应的试剂接触以在所述孔内产生表面改性和围绕所述孔的表面改性; （b）抛光所述基底以产生围绕所述孔的抛光表面修饰，其中去除围绕所述孔的所述表面改性并保留所述孔内的所述表面改性，和（c）将生物聚合物沉积到所述基底上，其中不同的亲和力 所述孔和所述抛光表面内的所述表面改性促进所述生物聚合物在所述孔内的定位。