公开(公告)号：US20210355534A1

公开(公告)日：2021-11-18

申请号：US17329482

申请日：2021-05-25

Applicant: Illumina, Inc.

Inventor： Eric Stava ,  Jens H. Gundlach ,  Jeffrey G. Mandell ,  Kevin L. Gunderson ,  Ian M. Derrington ,  Hosein Mohimani

IPC: C12Q1/6869 ,  G01N27/447 ,  C07K14/35

Abstract: Methods and compositions for characterizing a target polynucleotide, including, characterizing the sequence of the target polynucleotide, using the fractional translocation steps of the target polynucleotide's translocation through a pore.

公开(公告)号：US20240167085A1

公开(公告)日：2024-05-23

申请号：US18533863

申请日：2023-12-08

Applicant: Illumina, Inc.

Inventor： Eric Stava ,  Jens H. Gundlach ,  Jeffrey G. Mandell ,  Kevin L. Gunderson ,  Ian M. Derrington ,  Hosein Mohimani

IPC: C12Q1/6869 ,  C07K14/35 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  C07K14/35 ,  G01N27/447

Abstract: Methods and compositions for characterizing a target polynucleotide, including, characterizing the sequence of the target polynucleotide, using the fractional translocation steps of the target polynucleotide's translocation through a pore.

公开(公告)号：US11911765B2

公开(公告)日：2024-02-27

申请号：US16902826

申请日：2020-06-16

Applicant: ILLUMINA, INC.

Inventor： Tony Yen ,  Eric Stava ,  Rajagopal Panchapakesan

IPC: B01L3/00 ,  C12Q1/6869 ,  G06T7/00

CPC classification number: B01L3/527 ,  C12Q1/6869 ,  G06T7/0012 ,  B01L2300/0861 ,  B01L2300/0877 ,  G06T2207/30024

Abstract: A method includes flowing an incorporation reagent through a reagent management system and a flow cell of an instrument. The flow cell having a first polynucleotide positioned therein. The incorporation reagent adding a first base onto a sequence of bases. The sequence of bases includes a second polynucleotide complementary to the first polynucleotide. An image of an identification signal emanating from the first base is captured after the first base has been added onto the second polynucleotide. A cleavage reagent is flowed through the reagent management system and flow cell to remove a first terminator from the first base in order to enable a subsequent base in the sequence of bases to be added to the second polynucleotide. A buffer reagent is flowed through the reagent management system and flow cell in a plurality of cycles of consecutive forward and reverse flow directions.

公开(公告)号：US11041196B2

公开(公告)日：2021-06-22

申请号：US16439022

申请日：2019-06-12

Applicant: Illumina, Inc.

Inventor： Eric Stava ,  Jens H. Gundlach ,  Jeffrey G. Mandell ,  Kevin L. Gunderson ,  Ian M. Derrington ,  Hosein Mohimani

IPC: C12Q1/6869 ,  G01N27/447 ,  C07K14/35

Abstract: Methods and compositions for characterizing a target polynucleotide, including, characterizing the sequence of the target polynucleotide, using the fractional translocation steps of the target polynucleotide's translocation through a pore.

公开(公告)号：US11879155B2

公开(公告)日：2024-01-23

申请号：US17329482

申请日：2021-05-25

Applicant: Illumina, Inc.

Inventor： Eric Stava ,  Jens H. Gundlach ,  Jeffrey G. Mandell ,  Kevin L. Gunderson ,  Ian M. Derrington ,  Hosein Mohimani

IPC: C12Q1/6869 ,  G01N27/447 ,  C07K14/35

CPC classification number: C12Q1/6869 ,  C07K14/35 ,  G01N27/447 ,  C12Q1/6869 ,  C12Q2521/513 ,  C12Q2565/631

Abstract: Methods and compositions for characterizing a target polynucleotide, including, characterizing the sequence of the target polynucleotide, using the fractional translocation steps of the target polynucleotide's translocation through a pore.

公开(公告)号：US10364462B2

公开(公告)日：2019-07-30

申请号：US15606354

申请日：2017-05-26

Applicant: Illumina, Inc.

Inventor： Eric Stava ,  Jens H. Gundlach ,  Jeffrey G. Mandell ,  Kevin L. Gunderson ,  Ian M. Derrington ,  Hosein Mohimani

IPC: C12Q1/6869 ,  G01N27/447 ,  C07K14/35

Abstract: Methods and compositions for characterizing a target polynucleotide, including, characterizing the sequence of the target polynucleotide, using the fractional translocation steps of the target polynucleotide's translocation through a pore.

公开(公告)号：US20200024657A1

公开(公告)日：2020-01-23

申请号：US16439022

申请日：2019-06-12

Applicant: Illumina, Inc.

Inventor： Eric Stava ,  Jens H. Gundlach ,  Jeffrey G. Mandell ,  Kevin L. Gunderson ,  Ian M. Derrington ,  Hosein Mohimani

IPC: C12Q1/6869 ,  G01N27/447 ,  C07K14/35

Abstract: Methods and compositions for characterizing a target polynucleotide, including, characterizing the sequence of the target polynucleotide, using the fractional translocation steps of the target polynucleotide's translocation through a pore.

公开(公告)号：US20170268055A1

公开(公告)日：2017-09-21

申请号：US15606354

申请日：2017-05-26

Applicant: Illumina, Inc.

Inventor： Eric Stava ,  Jens H. Gundlach ,  Jeffrey G. Mandell ,  Kevin L. Gunderson ,  Ian M. Derrington ,  Hosein Mohimani

IPC: C12Q1/68 ,  C07K14/35 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  C07K14/35 ,  G01N27/447 ,  C12Q2521/513 ,  C12Q2565/631

Abstract: Methods and compositions for characterizing a target polynucleotide, including, characterizing the sequence of the target polynucleotide, using the fractional translocation steps of the target polynucleotide's translocation through a pore.

公开(公告)号：US09689033B2

公开(公告)日：2017-06-27

申请号：US14554741

申请日：2014-11-26

Applicant: Illumina, Inc.

Inventor： Eric Stava ,  Jens H. Gundlach ,  Jeffrey G. Mandell ,  Kevin L. Gunderson ,  Ian M. Derrington ,  Hosein Mohimani

IPC: C12Q1/68 ,  G01N27/00 ,  C12M1/34 ,  C12Q1/34 ,  G01N27/447 ,  C07K14/35

CPC classification number: C12Q1/6869 ,  C07K14/35 ,  G01N27/447 ,  C12Q2521/513 ,  C12Q2565/631

Abstract: Methods and compositions for characterizing a target polynucleotide, including, characterizing the sequence of the target polynucleotide, using the fractional translocation steps of the target polynucleotide's translocation through a pore.