公开(公告)号：US20250011887A1

公开(公告)日：2025-01-09

申请号：US18898408

申请日：2024-09-26

Applicant: ILLUMINA, INC.

Inventor： Brian Hawks ,  Stephen Gross ,  Gary Schroth

IPC: C12Q1/70 ,  C12Q1/6806 ,  C12Q1/6832 ,  C12Q1/6874

Abstract: Described herein are compositions and methods for enriching library fragments prepared for coronavirus sequences prepared from various samples. These methods may incorporate microfluidics and flowcells for greater case of use. Libraries enriched with the present methods may be used for sequencing. Also described are probes and methods for enzymatic depletion of unwanted RNA.

公开(公告)号：US20240018584A1

公开(公告)日：2024-01-18

申请号：US18359299

申请日：2023-07-26

Applicant: ILLUMINA, INC.

Inventor： Lena Christiansen ,  Frank J. Steemers ,  Gary Schroth ,  Jerushah Thomas ,  Dmitry K. Pokholok

IPC: C12Q1/6874 ,  C12Q1/6858 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  C12Q1/6858 ,  B01L3/502761 ,  C12Q1/6806

Abstract: Embodiments of systems, methods, and compositions provided herein relate to on bead tagmentation and droplet indexing. Some embodiments include performing co-assays on partitioned beads, including nucleic acid sequencing, indexed PCR, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

公开(公告)号：US20230183682A1

公开(公告)日：2023-06-15

申请号：US18163405

申请日：2023-02-02

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Niall Anthony Gormley ,  Andrew B. Kennedy ,  Robert Scott Kuersten ,  Gary Schroth ,  Carlo Randise-Hinchliff ,  Sarah E. Shultzaberger ,  Fiona Kaper ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Foad Mashayekhi

IPC: C12N15/10

CPC classification number: C12N15/1065

Abstract: This application describes methods of preparing an immobilized library of tagged RNA fragments. Also described herein are a number of methods of preparing DNA and RNA sequencing libraries from a single sample. These methods can include library preparation from single cells.