公开(公告)号：US12005437B2

公开(公告)日：2024-06-11

申请号：US16884852

申请日：2020-05-27

Applicant: Illumina, Inc.

Inventor： Nicholas Watson ,  Wesley A. Cox-Muranami ,  Cyril Delattre ,  Minsoung Rhee

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/14 ,  B01L2400/0475 ,  B01L2400/06

Abstract: Two-phase flushing systems and methods. An example method includes moving a valve to a first position to fluidly connect a first reagent reservoir containing a first reagent to a flow cell and flowing the first reagent from the first reagent reservoir to the flow cell to perform a biochemical reaction. The method includes moving the valve to a second position to fluidly connect a gas to the flow cell and flowing gas into the flow cell to expel at least a portion of the first reagent from the biochemical reaction from the flow cell. The method includes moving the valve to a third position to fluidly connect a buffer reagent reservoir containing a buffer reagent to the flow cell and flowing the buffer reagent into the flow cell.

公开(公告)号：US12011713B2

公开(公告)日：2024-06-18

申请号：US17817204

申请日：2022-08-03

Applicant: Illumina, Inc.

Inventor： Wesley A. Cox-Muranami ,  Kay Klausing ,  Bradley Kent Drews ,  Nicholas Watson ,  Jennifer Olivia Foley ,  Murphy Hitchcock ,  Paul Sangiorgio ,  Sz-Chin Steven Lin

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/06 ,  B01L2400/0487 ,  B01L2400/0644

Abstract: A system includes a fluidic device, a flow control valve, a first reagent fluid reservoir fluidly connectable to the fluidic device by the flow control valve, a first fluid buffer reservoir fluidly connectable to the fluidic device by the flow control valve, and a common fluid buffer source fluidly connectable to the fluidic device by the flow control valve. The flow control valve permits flow comprising: (i) flow from the first reagent fluid reservoir to the fluidic device, (ii) flow from the common fluid buffer source to the fluidic device, (iii) flow from the fluidic device to the first fluid buffer reservoir, (iv) flow from the first reagent fluid reservoir to the fluidic device, and (v) flow from the first fluid buffer reservoir to the fluidic device.

公开(公告)号：US20240299930A1

公开(公告)日：2024-09-12

申请号：US18668787

申请日：2024-05-20

Applicant: ILLUMINA, INC.

Inventor： Nicholas Watson ,  Wesley A. Cox-Muranami ,  Cyril Delattre ,  Minsoung Rhee

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/14 ,  B01L2400/0475 ,  B01L2400/06

Abstract: Two-phase flushing systems and methods. An example method includes moving a valve to a first position to fluidly connect a first reagent reservoir containing a first reagent to a flow cell and flowing the first reagent from the first reagent reservoir to the flow cell to perform a biochemical reaction. The method includes moving the valve to a second position to fluidly connect a gas to the flow cell and flowing gas into the flow cell to expel at least a portion of the first reagent from the biochemical reaction from the flow cell. The method includes moving the valve to a third position to fluidly connect a buffer reagent reservoir containing a buffer reagent to the flow cell and flowing the buffer reagent into the flow cell.

公开(公告)号：US11426723B2

公开(公告)日：2022-08-30

申请号：US16584353

申请日：2019-09-26

Applicant: Illumina, Inc.

Inventor： Wesley A. Cox-Muranami ,  Kay Klausing ,  Bradley Kent Drews ,  Nicholas Watson ,  Jennifer Olivia Foley ,  Murphy Hitchcock ,  Paul Sangiorgio ,  Sz-Chin Steven Lin

IPC: B01L3/00

Abstract: A system includes a fluidic device, a flow control valve, a first reagent fluid reservoir fluidly connectable to the fluidic device by the flow control valve, a first fluid buffer reservoir fluidly connectable to the fluidic device by the flow control valve, and a common fluid buffer source fluidly connectable to the fluidic device by the flow control valve. The flow control valve permits flow comprising: (i) flow from the first reagent fluid reservoir to the fluidic device, (ii) flow from the common fluid buffer source to the fluidic device, (iii) flow from the fluidic device to the first fluid buffer reservoir, (iv) flow from the first reagent fluid reservoir to the fluidic device, and (v) flow from the first fluid buffer reservoir to the fluidic device.