公开(公告)号：US11885323B2

公开(公告)日：2024-01-30

申请号：US16874934

申请日：2020-05-15

Applicant: Illumina, Inc.

Inventor： Cameron Mar ,  Wesley A. Cox-Muranami ,  Steven Hendrix ,  Cyril Delattre

IPC: F04B43/14 ,  F04B43/12 ,  F04B43/06

CPC classification number: F04B43/1223 ,  F04B43/06

Abstract: Linear peristaltic pumps for use with fluidic cartridges. An apparatus includes a reagent cartridge configured to be received within a cartridge receptacle of a system. The reagent cartridge includes a reagent reservoir and a body including a surface that forms depressions. Each depression has a fluid inlet and a fluid outlet and is fluidly coupled to at least one other depression. The reagent cartridge also includes a deformable material coupled to the surface of the body and includes portions. Each portion covers one of the depressions to define chambers. The portions of the deformable material are movable relative to the depressions between a first position outside of a dimensional envelope of the body and a second position within the dimensional envelope of the body.

公开(公告)号：US20220291250A1

公开(公告)日：2022-09-15

申请号：US17831222

申请日：2022-06-02

Applicant: Illumina, Inc.

Inventor： Wesley A. Cox-Muranami ,  James Osmus ,  Paul Crivelli ,  Bradley Kent Drews

IPC: G01N35/10

Abstract: An apparatus includes a fluidic circuit, a bypass fluidic circuit, a first set of fluid wells, a second set of fluid wells, a first valve, and a second valve. The first valve operatively associated with the first set of fluid wells such that the first selectively fluidly connects any one of the first set of fluid wells to a first valve outlet. The second valve operatively associated with the fluidic circuit, the bypass fluidic circuit, the first valve outlet, and the second set of fluid wells such that the second valve selectively fluidly connects any one of the second set of fluid wells and the first valve outlet to the fluidic circuit or the first valve outlet to the bypass fluidic circuit.

公开(公告)号：US20240299930A1

公开(公告)日：2024-09-12

申请号：US18668787

申请日：2024-05-20

Applicant: ILLUMINA, INC.

Inventor： Nicholas Watson ,  Wesley A. Cox-Muranami ,  Cyril Delattre ,  Minsoung Rhee

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/14 ,  B01L2400/0475 ,  B01L2400/06

Abstract: Two-phase flushing systems and methods. An example method includes moving a valve to a first position to fluidly connect a first reagent reservoir containing a first reagent to a flow cell and flowing the first reagent from the first reagent reservoir to the flow cell to perform a biochemical reaction. The method includes moving the valve to a second position to fluidly connect a gas to the flow cell and flowing gas into the flow cell to expel at least a portion of the first reagent from the biochemical reaction from the flow cell. The method includes moving the valve to a third position to fluidly connect a buffer reagent reservoir containing a buffer reagent to the flow cell and flowing the buffer reagent into the flow cell.

公开(公告)号：US11426723B2

公开(公告)日：2022-08-30

申请号：US16584353

申请日：2019-09-26

Applicant: Illumina, Inc.

Inventor： Wesley A. Cox-Muranami ,  Kay Klausing ,  Bradley Kent Drews ,  Nicholas Watson ,  Jennifer Olivia Foley ,  Murphy Hitchcock ,  Paul Sangiorgio ,  Sz-Chin Steven Lin

IPC: B01L3/00

Abstract: A system includes a fluidic device, a flow control valve, a first reagent fluid reservoir fluidly connectable to the fluidic device by the flow control valve, a first fluid buffer reservoir fluidly connectable to the fluidic device by the flow control valve, and a common fluid buffer source fluidly connectable to the fluidic device by the flow control valve. The flow control valve permits flow comprising: (i) flow from the first reagent fluid reservoir to the fluidic device, (ii) flow from the common fluid buffer source to the fluidic device, (iii) flow from the fluidic device to the first fluid buffer reservoir, (iv) flow from the first reagent fluid reservoir to the fluidic device, and (v) flow from the first fluid buffer reservoir to the fluidic device.

公开(公告)号：US11291998B2

公开(公告)日：2022-04-05

申请号：US17165757

申请日：2021-02-02

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Wesley A. Cox-Muranami ,  Darren Robert Segale ,  Oliver Jon Miller ,  Jennifer Olivia Foley ,  Tarun Kumar Khurana ,  Paul Crivelli ,  Gary Watts

IPC: B01L3/00 ,  G01N1/31 ,  B01L9/00

Abstract: An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

公开(公告)号：US12000854B2

公开(公告)日：2024-06-04

申请号：US17831222

申请日：2022-06-02

Applicant: Illumina, Inc.

Inventor： Wesley A. Cox-Muranami ,  James Osmus ,  Paul Crivelli ,  Bradley Kent Drews

IPC: G01N35/10

CPC classification number: G01N35/1097

Abstract: An apparatus includes a fluidic circuit, a bypass fluidic circuit, a first set of fluid wells, a second set of fluid wells, a first valve, and a second valve. The first valve operatively associated with the first set of fluid wells such that the first selectively fluidly connects any one of the first set of fluid wells to a first valve outlet. The second valve operatively associated with the fluidic circuit, the bypass fluidic circuit, the first valve outlet, and the second set of fluid wells such that the second valve selectively fluidly connects any one of the second set of fluid wells and the first valve outlet to the fluidic circuit or the first valve outlet to the bypass fluidic circuit.

公开(公告)号：US20230031325A1

公开(公告)日：2023-02-02

申请号：US17790058

申请日：2020-12-18

Applicant: ILLUMINA, INC.

Inventor： Paul Crivelli ,  Cyril Delattre ,  Gerald Kreindl ,  Wesley A. Cox-Muranami ,  Bryan Crane

IPC: B01L3/00

Abstract: Actuation systems and methods for use with flow cells. In accordance with an implementation, a method includes moving, using an actuator disposed within a manifold assembly, a membrane portion of a membrane of the manifold assembly away from a valve seat to enable fluidic flow from a reagent fluidic line to a common fluidic line. The membrane portion and the valve seat forming a membrane valve. The reagent fluidic line being fluidically coupled to a reagent reservoir. The common fluidic line being fluidically coupled to a flow cell. The common fluidic line has a common central axis and the reagent fluidic line has a reagent central axis that is non-collinear with the common central axis. The method includes urging the membrane portion against the valve seat to prevent fluidic flow from the reagent fluidic line to the common fluidic line.

公开(公告)号：US20210197200A1

公开(公告)日：2021-07-01

申请号：US17115454

申请日：2020-12-08

Applicant: ILLUMINA, INC.

Inventor： Wesley A. Cox-Muranami ,  Cameron Mar ,  Cyril Delattre ,  Paul Crivelli ,  Kamal Shah ,  Kirkpatrick W. Norton

IPC: B01L3/00 ,  G01N27/327

Abstract: Systems for monitoring fluidics in reagent cartridges and related methods. An apparatus includes a system includes a reagent cartridge receptacle and a flow cell assembly. The apparatus includes a reagent cartridge receivable within the reagent cartridge receptacle and adapted to carry the flow cell assembly. The reagent cartridge includes a reagent reservoir fluidically coupled to the flow cell assembly. The apparatus includes a sensor module adapted to be positioned adjacent the reagent reservoir. The sensor module is adapted to generate a signal associated with a volume of reagent contained within the reagent reservoir.

公开(公告)号：US12005437B2

公开(公告)日：2024-06-11

申请号：US16884852

申请日：2020-05-27

Applicant: Illumina, Inc.

Inventor： Nicholas Watson ,  Wesley A. Cox-Muranami ,  Cyril Delattre ,  Minsoung Rhee

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/14 ,  B01L2400/0475 ,  B01L2400/06

Abstract: Two-phase flushing systems and methods. An example method includes moving a valve to a first position to fluidly connect a first reagent reservoir containing a first reagent to a flow cell and flowing the first reagent from the first reagent reservoir to the flow cell to perform a biochemical reaction. The method includes moving the valve to a second position to fluidly connect a gas to the flow cell and flowing gas into the flow cell to expel at least a portion of the first reagent from the biochemical reaction from the flow cell. The method includes moving the valve to a third position to fluidly connect a buffer reagent reservoir containing a buffer reagent to the flow cell and flowing the buffer reagent into the flow cell.

公开(公告)号：US20230330671A1

公开(公告)日：2023-10-19

申请号：US18215794

申请日：2023-06-28

Applicant: ILLUMINA, INC.

Inventor： Sz-chin Steven Lin ,  Richard Lemoine ,  Rajagopal Panchapakesan ,  Wesley A. Cox-Muranami ,  Darren Segale

IPC: B01L3/00 ,  G01M3/26 ,  F16K99/00 ,  G01M3/28

CPC classification number: B01L3/502738 ,  G01M3/26 ,  F16K99/0028 ,  G01M3/2876 ,  F16K99/0013 ,  F16K2099/0084 ,  B01L2400/0644

Abstract: A method includes engaging a well of a cartridge with a flow sensor of an instrument. The cartridge includes: a rotary valve including a rotatable port and a center port; the well in fluid communication with a channel, the channel including a channel port that the rotatable port is to align to in order to receive fluid from the well; and a flowcell including an inlet gasket in fluid communication with the center port. A source of pressurized air is connected to the flow sensor in order to establish a mass flow of air through a flow path. The flow path extends through one of the flow sensor, the channel, the rotary valve, and the flowcell. The mass flow of air through the flow path is measured with the flow sensor. It is determined if there is one of an air leak and an air blockage within the flow path.