公开(公告)号：US20220150394A1

公开(公告)日：2022-05-12

申请号：US17513042

申请日：2021-10-28

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Geraint Evans ,  Stanley Hong ,  Merek Siu ,  Shaoping Lu ,  John Moon

IPC: H04N5/235 ,  H04N5/225 ,  G06K9/00 ,  G02B21/00

Abstract: A method is used to generate an analysis image of a moving sample based on one or more exposures. An illumination source illuminates a field of view of a camera for one or more pulses while the sample moves through the field of view. The distance moved by the sample during each of these one or more pulses may be less than the size of one pixel in an image captured by the camera.

公开(公告)号：US20200218050A1

公开(公告)日：2020-07-09

申请号：US16626113

申请日：2019-01-14

Applicant: ILLUMINA, INC.

Inventor： Peter Clarke Newman ,  Danilo Condello ,  Shaoping Lu ,  Simon Prince ,  Merek C. Siu ,  Stanley S. Hong ,  Aaron Liu

IPC: G02B21/06 ,  G01N21/64 ,  G02B21/18

Abstract: The disclosure provides for structured illumination microscopy (SIM) imaging systems. In one set of implementations, a SIM imaging system may be implemented as a multi-arm SIM imaging system, whereby each arm of the system includes a light emitter and a beam splitter (e.g., a transmissive diffraction grating) having a specific, fixed orientation with respect to the system's optical axis. In a second set of implementations, a SIM imaging system may be implemented as a multiple beam splitter slide SIM imaging system, where one linear motion stage is mounted with multiple beam splitters having a corresponding, fixed orientation with respect to the system's optical axis. In a third set of implementations, a SIM imaging system may be implemented as a pattern angle spatial selection SIM imaging system, whereby a fixed two-dimensional diffraction grating is used in combination with a spatial filter wheel to project one-dimensional fringe patterns on a sample.

公开(公告)号：US11226475B2

公开(公告)日：2022-01-18

申请号：US16621111

申请日：2019-01-14

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Peter Clarke Newman ,  Danilo Condello ,  Shaoping Lu ,  Simon Prince ,  Merek C. Siu ,  Stanley S. Hong ,  Aaron Liu ,  Gary Mark Skinner ,  Geraint Wyn Evans

IPC: G02B21/06 ,  G02B5/18 ,  G02B21/00 ,  G02B21/16 ,  G02B21/26 ,  G02B21/36 ,  G02B27/10 ,  G02B27/28 ,  G02B27/42 ,  H04N5/235 ,  G02B21/02

Abstract: The disclosure provides for structured illumination microscopy (SIM) imaging systems. In one set of implementations, a SIM imaging system may be implemented as a multi-arm SIM imaging system, whereby each arm of the system includes a light emitter and a beam splitter (e.g., a transmissive diffraction grating) having a specific, fixed orientation with respect to the system's optical axis. In a second set of implementations, a SIM imaging system may be implemented as a multiple beam splitter slide SIM imaging system, where one linear motion stage is mounted with multiple beam splitters having a corresponding, fixed orientation with respect to the system's optical axis. In a third set of implementations, a SIM imaging system may be implemented as a pattern angle spatial selection SIM imaging system, whereby a fixed two-dimensional diffraction grating is used in combination with a spatial filter wheel to project one-dimensional fringe patterns on a sample.

公开(公告)号：US10996453B2

公开(公告)日：2021-05-04

申请号：US16626113

申请日：2019-01-14

Applicant: ILLUMINA, INC.

Inventor： Peter Clarke Newman ,  Danilo Condello ,  Shaoping Lu ,  Simon Prince ,  Merek C. Siu ,  Stanley S. Hong ,  Aaron Liu

IPC: G02B21/06 ,  G01N21/64 ,  G02B21/18

Abstract: The disclosure provides for structured illumination microscopy (SIM) imaging systems. In one set of implementations, a SIM imaging system may be implemented as a multi-arm SIM imaging system, whereby each arm of the system includes a light emitter and a beam splitter (e.g., a transmissive diffraction grating) having a specific, fixed orientation with respect to the system's optical axis. In a second set of implementations, a SIM imaging system may be implemented as a multiple beam splitter slide SIM imaging system, where one linear motion stage is mounted with multiple beam splitters having a corresponding, fixed orientation with respect to the system's optical axis. In a third set of implementations, a SIM imaging system may be implemented as a pattern angle spatial selection SIM imaging system, whereby a fixed two-dimensional diffraction grating is used in combination with a spatial filter wheel to project one-dimensional fringe patterns on a sample.