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公开(公告)号：US10682829B2
公开(公告)日：2020-06-16
申请号：US15025866
申请日：2014-12-17
申请人： ILLUMINA, INC.
摘要： Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
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2.发明申请公开(公告)号：US20170175109A1
公开(公告)日：2017-06-22
申请号：US15451035
申请日：2017-03-06
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , David L. Barker , Jorge Velarde, Jr. , Steven M. Barnard , Michael Graige
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.
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3.发明申请COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURES USING HYBRIDIZATION 有权使用混合从复合混合物中代表性选择核酸的组合物和方法公开(公告)号：US20140045720A1
公开(公告)日：2014-02-13
申请号：US14046859
申请日：2013-10-04
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , David L. Barker , Jorge Velarde, JR. , Steven M. Barnard , Michael Graige
IPC分类号： C12N15/10
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.
摘要翻译： 本发明提供了从复杂混合物中选择核酸序列的表示样品的方法。 该方法包括：（a）在足以与包含与复合物混合物中共同存在的序列的预定部分的一个或多个核酸互补的捕获探针的杂交群的条件下使核酸的复杂混合物接触，以形成杂合复合物 具有探针群的一个或多个核酸，捕获探针的群体连接到固体支持物上，和（b）除去未杂交的核酸以选择复杂度小于10％但更多的核酸的代表性样品 超过0.001％的复合混合物，其中代表性样品包含核酸拷贝，其拷贝中的每个序列的比例相对于拷贝中的所有其它序列基本上与在一个或更多个预定部分中的序列的比例相同 复杂混合物中更多的核酸。 还提供了从完整基因组中选择基因组序列的表示样品的方法。 本发明还提供了一种核酸群体，其包括具有小于复合物混合物的10％但大于0.001％的复杂性的代表性样品，所述代表性样品包含核酸拷贝，所述核酸拷贝具有拷贝中每个序列的比例相对于 拷贝中的所有其它序列基本上与复合物混合物中一个或多个核酸中共存的序列的预定部分中的序列比例相同。
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公开(公告)号：US11173466B2
公开(公告)日：2021-11-16
申请号：US16118307
申请日：2018-08-30
申请人： Illumina, Inc.
发明人： Steven M. Barnard , M. Shane Bowen , Maria Candelaria Rogert Bacigalupo , Wayne N. George , Andrew A. Brown , James Tsay
IPC分类号： B01J19/00 , C12N15/10 , C12Q1/6837
摘要： An example method includes contacting a substrate coated with a sol-gel material with a stamp that includes a plurality of protruding features. While contacting the coated sol-gel material with the stamp, the example method further includes curing the coated sol-gel material so as to form a patterned sol-gel layer that includes a plurality of wells. The stamp is separated from the patterned sol-gel layer.
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公开(公告)号：US11110683B2
公开(公告)日：2021-09-07
申请号：US16880809
申请日：2020-05-21
申请人： ILLUMINA, INC.
摘要： Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
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6.发明申请SUBSTRATES COMPRISING NANO-PATTERNING SURFACES AND METHODS OF PREPARING THEREOF 审中-公开包含纳米花纹表面的基材及其制备方法公开(公告)号：US20160246170A1
公开(公告)日：2016-08-25
申请号：US15025866
申请日：2014-12-17
申请人： ILLUMINA, INC.
摘要： Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
摘要翻译： 公开了包含可官能化层的底物，包含多个微尺度或纳米尺度图案的聚合物层或其组合，以及背衬层及其通过使用室温UV纳米压花工艺的制备。 基材可以通过卷对卷连续方法制备。 底物可用作流动池，用于生物分子分析的纳流体或微流体装置。
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7.发明授权Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization 有权使用杂交从复杂混合物中代表性选择核酸的组合物和方法公开(公告)号：US09139826B2
公开(公告)日：2015-09-22
申请号：US14536354
申请日：2014-11-07
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , David L. Barker , Jorge Velarde, Jr. , Steven M. Barnard , Michael Graige
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.
摘要翻译： 本发明提供了从复杂混合物中选择核酸序列的表示样品的方法。 该方法包括：（a）在足以与包含与复合物混合物中共同存在的序列的预定部分的一个或多个核酸互补的捕获探针的杂交群的条件下使核酸的复杂混合物接触，以形成杂合复合物 具有探针群的一个或多个核酸，捕获探针的群体连接到固体支持物上，和（b）除去未杂交的核酸以选择复杂度小于10％但更多的核酸的代表性样品 超过0.001％的复合混合物，其中代表性样品包含核酸拷贝，其拷贝中的每个序列的比例相对于拷贝中的所有其它序列基本上与在一个或更多个预定部分中的序列的比例相同 复杂混合物中更多的核酸。 还提供了从完整基因组中选择基因组序列的表示样品的方法。 本发明还提供了一种核酸群体，其包括具有小于复合物混合物的10％但大于0.001％的复杂性的代表性样品，所述代表性样品包含核酸拷贝，所述核酸拷贝具有拷贝中每个序列的比例相对于 拷贝中的所有其它序列基本上与复合物混合物中一个或多个核酸中共存的序列的预定部分中的序列比例相同。
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8.发明授权Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization 有权使用杂交从复杂混合物中代表性选择核酸的组合物和方法公开(公告)号：US08916350B2
公开(公告)日：2014-12-23
申请号：US14046859
申请日：2013-10-04
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , David L. Barker , Jorge Velarde, Jr. , Steven M. Barnard , Michael Graige
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.
摘要翻译： 本发明提供了从复杂混合物中选择核酸序列的表示样品的方法。 该方法包括：（a）在足以与包含与复合物混合物中共同存在的序列的预定部分的一个或多个核酸互补的捕获探针的杂交群的条件下使核酸的复杂混合物接触，以形成杂合复合物 具有探针群的一个或多个核酸，捕获探针的群体连接到固体支持物上，和（b）除去未杂交的核酸以选择复杂度小于10％但更多的核酸的代表性样品 超过0.001％的复合混合物，其中代表性样品包含核酸拷贝，其拷贝中的每个序列的比例相对于拷贝中的所有其它序列基本上与在一个或更多个预定部分中的序列的比例相同 复杂混合物中更多的核酸。 还提供了从完整基因组中选择基因组序列的表示样品的方法。 本发明还提供了一种核酸群体，其包括具有小于复合物混合物的10％但大于0.001％的复杂性的代表性样品，所述代表性样品包含核酸拷贝，所述核酸拷贝具有拷贝中每个序列的比例相对于 拷贝中的所有其它序列基本上与复合物混合物中一个或多个核酸中共存的序列的预定部分中的序列比例相同。
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9.发明申请公开(公告)号：US20180073016A1
公开(公告)日：2018-03-15
申请号：US15818712
申请日：2017-11-20
申请人： ILLUMINA, INC.
CPC分类号： C12N15/1072 , C12Q1/6837 , C12Q2565/515
摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.
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10.发明申请公开(公告)号：US20160199832A1
公开(公告)日：2016-07-14
申请号：US14912394
申请日：2014-08-29
发明人： Arash Jamshidi , Yan-You Lin , Alex Aravanis , Cyril Delattre , Arnaud Rival , Jennifer Foley , Poorya Sabounchi , Tarun Khurana , Majid Babazadeh , Hamed Amini , Bala Murali Venkatesan , M. Shane Bowen , Steven M. Barnard , Maria Candelaria Rogert Bacigalupo , Dietrich Dehlinger
IPC分类号： B01L3/00
CPC分类号： B01L3/50273 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/502792 , B01L2200/06 , B01L2300/0636 , B01L2300/0819 , B01L2300/0858 , B01L2300/087 , B01L2300/161 , B01L2300/166 , B01L2400/0427 , B41J2/04 , G01N15/1463 , G01N15/1475 , G01N2015/1006
摘要： Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.
摘要翻译： 本文提供的液滴致动器包括（a）由液滴操作间隙分离的第一和第二基板，第一和第二基板包括面对液滴操作间隙的各个疏水表面; （b）耦合到第一衬底和第二衬底中的至少一个的多个电极，电极沿着液滴操作间隙排列，以控制液滴沿着液滴操作间隙内的疏水表面的运动; 和（c）暴露于液滴操作间隙的亲水或杂色亲水表面，当液滴在液滴操作间隙内的选择位置时，亲水或杂色亲水表面定位成接触液滴。
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