公开(公告)号：US20220243261A1

公开(公告)日：2022-08-04

申请号：US17575279

申请日：2022-01-13

申请人： Illumina, Inc.

发明人： Min-Jui Richard Shen ,  Jonathan Mark Boutell ,  Kathryn M. Stephens ,  Mostafa Ronaghi ,  Kevin L. Gunderson ,  Bala Murali Venkatesan ,  M. Shane Bowen ,  Kandaswamy Vijayan

IPC分类号： C12Q1/6848 ,  B01J19/00 ,  C12Q1/6844 ,  C12Q1/6874

摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

公开(公告)号：US20220098653A1

公开(公告)日：2022-03-31

申请号：US17548246

申请日：2021-12-10

申请人： ILLUMINA, INC.

发明人： M. Shane Bowen ,  Bala Murali Venkatesan ,  Hui Han ,  Sang Ryul Park

IPC分类号： C12Q1/6844 ,  C12Q1/6834 ,  C12N15/09 ,  B01J19/00 ,  B01L3/00 ,  C07K1/04 ,  C12Q1/6813 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B50/18

摘要： Structured substrate including (a) a plurality of nanoparticles distributed on a solid support, (b) a gel material forming a layer in association with the plurality of nanoparticles, and (c) a library of target nucleic acids in the gel material.

公开(公告)号：US11110452B2

公开(公告)日：2021-09-07

申请号：US16026847

申请日：2018-07-03

申请人： Illumina, Inc.

发明人： Jeffrey S. Fisher ,  John A. Moon ,  Bala Murali Venkatesan

IPC分类号： B01L3/00 ,  B81C1/00 ,  B29C65/14 ,  B29C65/16 ,  B29C65/00 ,  B81C3/00 ,  C12Q1/6874 ,  B29K507/04 ,  B29C65/82 ,  B29L31/00 ,  B29L9/00 ,  B29L31/34

摘要： A method of making a flowcell includes bonding a first surface of an organic solid support to a surface of a first inorganic solid support via a first bonding layer, wherein the organic solid support includes a plurality of elongated cutouts. The method further includes bonding a surface of a second inorganic solid support to a second surface of the organic solid support via a second bonding layer, so as to form the flowcell. The formed flowcell includes a plurality of channels defined by the surface of the first inorganic solid support, the surface of the second inorganic solid support, and walls of the elongated cutouts.

公开(公告)号：US10913975B2

公开(公告)日：2021-02-09

申请号：US15747670

申请日：2016-07-21

申请人： ILLUMINA, INC.

发明人： Alex So ,  Li Liu ,  Min-Jui Richard Shen ,  Neeraj Salathia ,  Kathryn M. Stephens ,  Anne Jager ,  Timothy Wilson ,  Justin Fullerton ,  Sean M. Ramirez ,  Shannon Kaplan ,  Rigo Pantoja ,  Bala Murali Venkatesan ,  Steven Modiano

IPC分类号： C12Q1/68 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6816 ,  C12Q1/6855

摘要： Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

公开(公告)号：US10385384B2

公开(公告)日：2019-08-20

申请号：US15672161

申请日：2017-08-08

申请人： Illumina, Inc.

发明人： Min-Jui Richard Shen ,  Jonathan Mark Boutell ,  Kathryn M. Stephens ,  Mostafa Ronaghi ,  Kevin L. Gunderson ,  Bala Murali Venkatesan ,  M. Shane Bowen ,  Kandaswamy Vijayan

IPC分类号： C12Q1/68 ,  B01J19/00 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6874

摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

公开(公告)号：US20160199832A1

公开(公告)日：2016-07-14

申请号：US14912394

申请日：2014-08-29

申请人： ILLUMINA, INC. ,  ADVANCED LIQUID LOGIC FRANCE SAS

发明人： Arash Jamshidi ,  Yan-You Lin ,  Alex Aravanis ,  Cyril Delattre ,  Arnaud Rival ,  Jennifer Foley ,  Poorya Sabounchi ,  Tarun Khurana ,  Majid Babazadeh ,  Hamed Amini ,  Bala Murali Venkatesan ,  M. Shane Bowen ,  Steven M. Barnard ,  Maria Candelaria Rogert Bacigalupo ,  Dietrich Dehlinger

IPC分类号： B01L3/00

CPC分类号： B01L3/50273 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2300/166 ,  B01L2400/0427 ,  B41J2/04 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006

摘要： Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.

摘要翻译： 本文提供的液滴致动器包括（a）由液滴操作间隙分离的第一和第二基板，第一和第二基板包括面对液滴操作间隙的各个疏水表面; （b）耦合到第一衬底和第二衬底中的至少一个的多个电极，电极沿着液滴操作间隙排列，以控制液滴沿着液滴操作间隙内的疏水表面的运动; 和（c）暴露于液滴操作间隙的亲水或杂色亲水表面，当液滴在液滴操作间隙内的选择位置时，亲水或杂色亲水表面定位成接触液滴。

公开(公告)号：US20210379585A1

公开(公告)日：2021-12-09

申请号：US17410943

申请日：2021-08-24

申请人： Illumina, Inc.

发明人： Jeffrey S. Fisher ,  John A. Moon ,  Bala Murali Venkatesan

IPC分类号： B01L3/00 ,  B81C1/00 ,  B29C65/14 ,  B29C65/16 ,  B29C65/00 ,  B81C3/00 ,  C12Q1/6874

摘要： A method of making a flowcell includes bonding a first surface of an organic solid support to a surface of a first inorganic solid support via a first bonding layer, wherein the organic solid support includes a plurality of elongated cutouts. The method further includes bonding a surface of a second inorganic solid support to a second surface of the organic solid support via a second bonding layer, so as to form the flowcell. The formed flowcell includes a plurality of channels defined by the surface of the first inorganic solid support, the surface of the second inorganic solid support, and walls of the elongated cutouts.

公开(公告)号：US11060135B2

公开(公告)日：2021-07-13

申请号：US16118268

申请日：2018-08-30

申请人： ILLUMINA, INC.

发明人： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC分类号： C12Q1/6844 ,  C40B50/18 ,  C12Q1/686 ,  B01J19/00 ,  C40B40/06

摘要： A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.

公开(公告)号：US20210130885A1

公开(公告)日：2021-05-06

申请号：US17145846

申请日：2021-01-11

申请人： ILLUMINA, INC.

发明人： Alex So ,  Li Liu ,  Min-Jui Richard Shen ,  Neeraj Salathia ,  Kathryn M. Stephens ,  Anne Jager ,  Timothy Wilson ,  Justin Fullerton ,  Sean M. Ramirez ,  Shannon Kaplan ,  Rigo Pantoja ,  Bala Murali Venkatesan ,  Steven Modiano

IPC分类号： C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6816 ,  C12Q1/6855

摘要： Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

公开(公告)号：US10682829B2

公开(公告)日：2020-06-16

申请号：US15025866

申请日：2014-12-17

申请人： ILLUMINA, INC.

发明人： M. Shane Bowen ,  Bala Murali Venkatesan ,  Steven M. Barnard

IPC分类号： B32B3/26 ,  B32B3/30 ,  G03F7/00 ,  C08G69/00 ,  B29C59/00 ,  B29C59/02 ,  B29K77/00 ,  B29K105/00

摘要： Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.