公开(公告)号：US20240167020A1

公开(公告)日：2024-05-23

申请号：US18549343

申请日：2022-03-08

Applicant: Illumina, Inc.

Inventor： Hongxia Xu ,  Tong Liu ,  Shi Min Xiao ,  Dan Cao ,  Victor Quijano ,  Kai-How Farh ,  Mohan Sun

IPC: C12N15/10

CPC classification number: C12N15/1082 ,  C12N15/1062 ,  C12N15/1065

Abstract: Analyzing expression of protein-coding variants in cells is provided herein. A method may include replacing a protein coding-region of the DNA in a cell with a donor vector including a variant of the protein-coding region and a first barcode identifying that variant. The cell may generate mRNA including an expression of the variant and an expression of the first barcode. A second barcode corresponding to the cell may be coupled to the mRNA. The mRNA. having the second barcode coupled thereto, may be reverse transcribed into complementary cDNA. The cDNA may be sequenced. The donor vector or cDNA may be sequenced using amplicon sequencing. The donor vector sequence and the cDNA sequence may be correlated to identify the variant and the cell's expression of the variant.

公开(公告)号：US20250115900A1

公开(公告)日：2025-04-10

申请号：US18948276

申请日：2024-11-14

Applicant: ILLUMINA, INC.

Inventor： Dan Cao ,  Jeffrey S. Fisher ,  Fiona Kaper ,  Tarun Khurana ,  Tong Liu ,  Burak Okumus ,  Victor Quijano ,  Clifford Lee Wang ,  Yir-Shyuan Wu ,  Shi Min Xiao ,  Hongxia Xu

IPC: C12N15/10

Abstract: An example of a biotin-streptavidin cleavage composition includes a formamide reagent and a salt buffer. The formamide reagent is present in the biotin-streptavidin cleavage composition in an amount ranging from about 10% to about 50%, based on a total volume of the biotin-streptavidin cleavage composition. The salt buffer makes up the balance of the biotin-streptavidin cleavage composition. In some examples, the biotin-streptavidin cleavage composition is used to cleave library fragments from a solid support. In other examples, other mechanisms are used to cleave library fragments from a solid support.